FastQCFastQC Report
Thu 26 May 2016
SRR805344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127274761
Sequences flagged as poor quality0
Sequence length59
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTA1978000.044.172471
ACTAAGC961050.042.2102934
CAAGTTA1781550.041.769611
GAGATCG807850.039.439193
TAAGCAG1509300.037.665016
TAAACTT1752500.035.6181141
CTAAGCA1105500.035.4651765
AGAGTAA1205500.035.0358352
CGTAGTA193900.034.8914381
CTCGTAT970150.034.69870816
GCTCGTA973050.034.5925715
AGATCGG978000.034.3145184
GATCGGA982800.034.2008555
AACTAAG1314100.034.1993373
TATGCCG979050.034.04223320
GAGTAAG733250.034.0117953
AAACTAA1600250.033.720952
TCGTATG1008000.033.4010417
ATGCCGT997950.033.2355321
TGCCGTC1008200.032.9291822
Produced by FastQC (version 0.11.5)