##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127274761
Sequences flagged as poor quality	0
Sequence length	59
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.790721539834596	33.0	31.0	34.0	30.0	34.0
2	24.491857281900533	31.0	16.0	34.0	3.0	34.0
3	27.511305654700855	31.0	16.0	34.0	16.0	34.0
4	34.18180723985017	35.0	32.0	37.0	32.0	37.0
5	35.079589518930625	35.0	35.0	37.0	33.0	37.0
6	35.4466757867257	37.0	35.0	37.0	33.0	37.0
7	35.44513089284057	37.0	35.0	37.0	33.0	37.0
8	35.83460238436433	37.0	35.0	37.0	35.0	37.0
9	37.71456498747619	39.0	37.0	39.0	35.0	39.0
10	37.81231163341175	39.0	38.0	39.0	35.0	39.0
11	37.839983097670085	39.0	38.0	39.0	35.0	39.0
12	37.768857872771804	39.0	38.0	39.0	35.0	39.0
13	37.612212644422094	39.0	38.0	39.0	35.0	39.0
14	38.56769929428506	40.0	39.0	40.0	36.0	40.0
15	38.65878191670696	40.0	39.0	40.0	36.0	40.0
16	38.67732751036162	40.0	39.0	40.0	36.0	40.0
17	38.66422048908817	40.0	39.0	40.0	36.0	40.0
18	38.664229579657196	40.0	39.0	40.0	36.0	40.0
19	38.74732691110691	40.0	39.0	40.0	36.0	40.0
20	38.73600991480157	40.0	39.0	40.0	36.0	40.0
21	38.700995950014004	40.0	39.0	40.0	36.0	40.0
22	38.67657956945604	40.0	39.0	40.0	36.0	40.0
23	38.61201593613678	40.0	39.0	40.0	36.0	40.0
24	38.47513806763306	40.0	39.0	40.0	35.0	40.0
25	38.61268551115174	40.0	39.0	40.0	36.0	40.0
26	38.53899703649807	40.0	39.0	40.0	36.0	40.0
27	38.5217605319251	40.0	39.0	40.0	36.0	40.0
28	38.503563970550296	40.0	39.0	40.0	35.0	40.0
29	38.39877110435116	40.0	38.0	40.0	35.0	40.0
30	38.239455684383486	40.0	38.0	40.0	35.0	40.0
31	38.224923997303755	40.0	38.0	40.0	34.0	40.0
32	38.17437757357093	40.0	38.0	40.0	34.0	40.0
33	37.849247998195025	40.0	38.0	40.0	34.0	40.0
34	37.957786823107845	40.0	38.0	40.0	34.0	40.0
35	37.967542472933815	40.0	38.0	40.0	34.0	40.0
36	31.970601806904984	39.0	26.0	40.0	10.0	40.0
37	33.032101337043564	39.0	25.0	40.0	16.0	40.0
38	35.79439152119092	39.0	33.0	40.0	30.0	40.0
39	37.0197655920171	39.0	36.0	40.0	31.0	40.0
40	37.4883025551311	39.0	37.0	40.0	33.0	40.0
41	37.72687303651664	40.0	38.0	40.0	33.0	40.0
42	37.770745238327336	40.0	38.0	40.0	33.0	40.0
43	37.77460971229009	40.0	38.0	40.0	33.0	40.0
44	37.68564226178354	40.0	38.0	40.0	33.0	40.0
45	30.261170280256902	39.0	24.0	40.0	3.0	40.0
46	32.263125695439335	39.0	24.0	40.0	15.0	40.0
47	35.029188002168006	39.0	30.0	40.0	26.0	40.0
48	36.29515760787797	39.0	35.0	40.0	30.0	40.0
49	36.971220908440756	39.0	37.0	40.0	31.0	40.0
50	37.141384944341006	40.0	37.0	40.0	32.0	40.0
51	29.34057773638247	38.0	18.0	40.0	3.0	40.0
52	31.71291513169685	38.0	22.0	40.0	12.0	40.0
53	34.20386651521585	38.0	30.0	40.0	23.0	40.0
54	35.961779327167626	38.0	34.0	40.0	30.0	40.0
55	36.59233295279965	39.0	36.0	40.0	31.0	40.0
56	36.80479656135437	39.0	36.0	40.0	31.0	40.0
57	36.75086151605502	39.0	36.0	40.0	31.0	40.0
58	36.608457689423595	39.0	36.0	40.0	31.0	40.0
59	35.967185544351565	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	1.0
7	1.0
8	22.0
9	64.0
10	137.0
11	218.0
12	328.0
13	655.0
14	1232.0
15	2799.0
16	5750.0
17	10836.0
18	17314.0
19	26741.0
20	42614.0
21	72191.0
22	123867.0
23	204767.0
24	322754.0
25	484112.0
26	706241.0
27	998929.0
28	1389163.0
29	1890169.0
30	2550130.0
31	3566834.0
32	5354390.0
33	9850626.0
34	1.5950423E7
35	4998875.0
36	7013986.0
37	1.2219991E7
38	2.9858347E7
39	2.9610253E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.160390369933594	28.568836204689475	23.81463831623302	33.45613510914391
2	18.37130143972535	44.101343077752865	22.745910322314415	14.78144516020737
3	23.111295412293096	31.82542609528059	25.043680891296273	20.019597601130044
4	22.982453685377575	32.49349649142142	25.152155657946984	19.37189416525402
5	25.15675044166848	24.60026461962871	28.279727824434886	21.963257114267925
6	20.420349483115512	24.638993429341426	35.423746739544065	19.516910347999
7	22.831823663766297	28.820377042389417	27.46713938044637	20.88065991339791
8	22.53414563473429	28.592551825730787	27.108687322539932	21.764615216994986
9	23.14505858706739	26.341924146296375	28.61323228098617	21.89978498565006
10	22.208718191975233	27.78288540647898	26.60256890995065	23.405827491595133
11	21.88622298807538	28.876806140692736	26.902444546723604	22.334526324508282
12	22.988086381085406	27.58707910675236	26.95439907367023	22.470435438492004
13	22.881734580511214	27.53555514435419	27.23038780642456	22.352322468710035
14	23.360099650864793	28.097064743260447	26.531171407974597	22.011664197900163
15	23.479878308315975	27.11290182662374	26.655891343610538	22.75132852144975
16	22.23434149681884	27.504359642836025	27.485945937073886	22.775352923271253
17	22.96629808638965	27.759005573775937	27.025831932224175	22.24886440761024
18	22.126648503390236	27.24938371716919	27.495504784330336	23.12846299511024
19	23.090662884843287	27.245191212733843	26.704338498031042	22.959807404391828
20	22.41414226658811	28.01684931076005	27.29057491610611	22.278433506545735
21	22.46613215011262	28.569912616060616	26.729687592970613	22.23426764085615
22	22.2849713306474	26.864554866459343	27.61445374075383	23.23602006213942
23	23.578333806496012	27.37540870337993	26.856527351876153	22.189730138247914
24	22.249819035213118	26.999520352664426	26.89097015864756	23.859690453474904
25	22.606838758864374	27.7514801225987	26.832221668835032	22.809459449701894
26	23.906878128021	26.757510076958617	27.229493677854954	22.10611811716543
27	22.883485909669083	27.401262218830645	27.40055508727296	22.314696784227316
28	23.75302594361187	26.642108563849515	26.894464174244252	22.710401318294362
29	22.659580558945226	26.8548325932429	28.33394045815572	22.151646389656154
30	22.588079344340706	27.432006727555358	27.37733838683068	22.60257554127326
31	22.623576562834796	26.837585654550942	26.90415973360186	23.634678049012404
32	22.58155644857192	27.449171167565577	27.752206110997925	22.217066272864578
33	23.197792530130933	26.769033178541974	27.45024836463845	22.582925926688638
34	23.13707114327247	26.923179215398406	27.074071661387762	22.865677979941363
35	23.252729580847532	26.85978408555016	27.58977799219753	22.297708341404782
36	31.248201676057363	25.329420968231087	24.09689773450056	19.325479621210995
37	23.4047793654863	27.0582319144956	27.054332476805833	22.482656243212272
38	22.811101566319188	27.646739010572567	27.23203856576089	22.310120857347357
39	22.907742093501163	27.56948567359714	27.05739357074888	22.465378662152823
40	22.73845794139814	27.71489235010231	27.064038250285932	22.48261145821362
41	22.636619211565442	27.7299385382464	27.1752244736095	22.458217776578657
42	22.693119808726255	27.574483522306515	27.190322518067823	22.542074150899406
43	22.683029827099812	27.672859664611742	27.055842595532354	22.588267912756088
44	22.749458551330534	27.629433144250807	27.169864416402245	22.451243888016414
45	20.710194851593553	39.81263417968626	21.60119868541729	17.875972283302893
46	22.692547817866263	27.54690696296024	27.08685974275764	22.673685476415862
47	22.770004651589957	27.51481733287246	27.200743280122914	22.514434735414667
48	22.939852151833936	27.36818810447422	27.074066161475645	22.617893582216194
49	23.019598520401072	27.4109208502069	27.050925674101244	22.51855495529078
50	22.899176373232397	27.39748535061087	27.163889940441532	22.5394483357152
51	17.840901700848608	43.577047455622406	21.023981337509642	17.558069506019343
52	22.825827973858857	27.3812213247841	27.07355702675411	22.719393674602934
53	23.115245134893634	27.260466825783315	27.0082652129278	22.616022826395252
54	22.804728739580977	27.260851819631387	27.13702444116159	22.797394999626043
55	22.728217890741117	27.40557100712214	27.14412875621114	22.7220823459256
56	22.86102898280045	27.267469785309594	27.167463311913036	22.704037919976923
57	22.94151783950315	27.233022264327804	27.0722873327572	22.753172563411848
58	22.867192027176543	27.288511663361128	27.042240527169405	22.802055782292925
59	22.9174871520678	27.30404420087656	27.015620166829464	22.76284848022618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6710.0
1	5096.0
2	3482.0
3	3459.5
4	3605.0
5	3773.0
6	4050.0
7	4327.0
8	5040.5
9	7423.0
10	9092.0
11	12985.5
12	16879.0
13	23699.0
14	43264.0
15	56009.0
16	84234.0
17	112459.0
18	175753.5
19	367129.5
20	495211.0
21	588762.0
22	682313.0
23	836153.5
24	989994.0
25	1144072.0
26	1505748.0
27	1713346.0
28	1996045.5
29	2278745.0
30	2583679.0
31	3197383.5
32	3506154.0
33	3903092.0
34	4300030.0
35	4695358.5
36	5451286.5
37	5811886.0
38	6290387.5
39	6768889.0
40	7266027.5
41	7763166.0
42	8116387.0
43	8685890.0
44	8902172.0
45	8965224.0
46	9028276.0
47	9039230.0
48	8923569.0
49	8796954.0
50	8516676.0
51	8236398.0
52	7769253.5
53	6769416.5
54	6236724.0
55	5685578.5
56	5134433.0
57	4499123.5
58	3309820.5
59	2755827.0
60	2364735.5
61	1973644.0
62	1669449.0
63	1365254.0
64	1115743.0
65	707872.0
66	549512.0
67	428008.0
68	306504.0
69	235016.0
70	133427.5
71	103327.0
72	72507.5
73	41688.0
74	31731.0
75	16727.5
76	11681.0
77	9194.0
78	6707.0
79	5332.0
80	3957.0
81	3286.5
82	2138.0
83	1660.0
84	1154.5
85	649.0
86	509.0
87	267.0
88	165.0
89	141.0
90	117.0
91	99.5
92	60.5
93	39.0
94	33.5
95	28.0
96	19.5
97	10.5
98	10.0
99	22.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.27274761E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.511305057928844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.6777625083652	22.612804498409005
2	16.755952485789827	11.900514805180872
3	6.956862123183484	7.411417593069578
4	3.4215030553273142	4.860081550174553
5	2.1239954410242796	3.771292502393165
6	1.3785955309936129	2.937343587156695
7	0.9925411520737482	2.4672502143687307
8	0.7255559993019182	2.061235234225666
9	0.5346622793110768	1.708789977322488
>10	3.0840528802921465	19.64719507119842
>50	0.2000895771465647	4.878318737729768
>100	0.1334855642333742	9.326054942922857
>500	0.008611276192976823	2.0849563685230694
>1k	0.006263843770698804	4.154975989596491
>5k	5.947476426020411E-5	0.12830651785108496
>10k+	6.808229264057439E-6	0.049462409877948116
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0016609734588305374	0.0	0.0	0.0	7.857017307618436E-7
2	0.00321744858746975	0.0	0.0	5.499912115332906E-6	7.857017307618436E-7
3	0.038186675518487125	7.857017307618436E-7	0.0	1.4142631153713185E-5	3.1428069230473746E-6
4	0.5280316338602278	1.5714034615236873E-6	0.0	1.728543807676056E-5	3.9285086538092185E-6
5	0.5420564097543267	1.5714034615236873E-6	0.0	2.0428244999807935E-5	3.9285086538092185E-6
6	0.6717184092767615	1.5714034615236873E-6	0.0	2.2785350192093466E-5	6.285613846094749E-6
7	0.6718692640090678	2.357105192285531E-6	0.0	2.592815711514084E-5	7.071315576856593E-6
8	0.671884978043683	5.499912115332906E-6	0.0	2.592815711514084E-5	7.071315576856593E-6
9	0.671921120323298	5.499912115332906E-6	0.0	2.9856665768950058E-5	8.64271903838028E-6
10	0.6719894763738743	7.857017307618437E-6	0.0	3.2999472691997435E-5	8.64271903838028E-6
11	0.6720028333032972	1.0214122499903968E-5	0.0	3.614227961504481E-5	1.0214122499903968E-5
12	0.6720138331275279	1.0214122499903968E-5	0.0	4.007078826885403E-5	1.1785525961427655E-5
13	0.6720287614604125	1.1785525961427655E-5	0.0	4.6356402114948776E-5	1.1785525961427655E-5
14	0.6720421183898354	1.2571227692189498E-5	0.0	4.714210384571062E-5	1.4142631153713185E-5
15	0.6720664751434889	1.3356929422951342E-5	0.0	5.107061249951984E-5	1.8071139807522405E-5
16	0.6721010460196425	1.4928332884475029E-5	0.0	5.7356226345614585E-5	1.9642543269046092E-5
17	0.6721332597906037	1.6499736345998718E-5	0.0	6.049903326866196E-5	2.592815711514084E-5
18	0.6722157584723337	1.6499736345998718E-5	0.0	6.128473499942381E-5	2.7499560576664527E-5
19	0.6727838208236745	1.6499736345998718E-5	0.0	6.364184019170933E-5	2.9070964038188214E-5
20	0.6729417468715577	1.8071139807522405E-5	0.0	6.83560505762804E-5	3.221377096123559E-5
21	0.673409239401361	1.9642543269046092E-5	0.0	7.228455923008962E-5	3.2999472691997435E-5
22	0.6736095933427052	2.121394673056978E-5	0.0	7.699876961466068E-5	3.5356577884282966E-5
23	0.6736842350071276	2.357105192285531E-5	0.0	7.857017307618437E-5	3.9285086538092184E-5
24	0.6740739430655854	2.357105192285531E-5	0.0	7.93558748069462E-5	4.085648999961587E-5
25	0.6742106551667381	2.357105192285531E-5	0.0	1.0685543538361073E-4	4.32135951919014E-5
26	0.6743222248125063	3.378517442275928E-5	7.857017307618436E-7	1.0921254057589627E-4	4.6356402114948776E-5
27	0.674554006823081	3.5356577884282966E-5	1.5714034615236873E-6	1.2335517172960944E-4	4.6356402114948776E-5
28	0.6746506481359646	3.614227961504481E-5	2.357105192285531E-6	1.2806938211418052E-4	4.714210384571062E-5
29	0.6747897173423095	3.692798134580665E-5	6.285613846094749E-6	1.3042648730646604E-4	4.949920903799615E-5
30	0.6749452862850004	3.849938480733034E-5	6.285613846094749E-6	1.312121890372279E-4	5.107061249951984E-5
31	0.6751558543488445	3.849938480733034E-5	6.285613846094749E-6	1.327835924987516E-4	5.107061249951984E-5
32	0.675550276617687	4.007078826885403E-5	9.428420769142124E-6	1.3592639942179897E-4	5.4999121153329054E-5
33	0.6762919790515262	4.1642191730377714E-5	9.428420769142124E-6	1.4064060980637001E-4	5.4999121153329054E-5
34	0.6766329736026768	4.32135951919014E-5	9.428420769142124E-6	1.8385420499827143E-4	5.4999121153329054E-5
35	0.677127179991326	4.6356402114948776E-5	9.428420769142124E-6	1.869970119213188E-4	6.914175230704224E-5
36	0.6775043168220917	4.714210384571062E-5	1.0999824230665811E-5	1.9878253788274645E-4	6.992745403780408E-5
37	0.678219305397085	4.714210384571062E-5	1.0999824230665811E-5	2.0271104653655567E-4	7.228455923008962E-5
38	0.6792627172955367	4.714210384571062E-5	1.1785525961427655E-5	2.0585385345960305E-4	7.621306788389883E-5
39	0.6808050497930223	4.792780557647246E-5	1.1785525961427655E-5	2.0899666038265042E-4	7.621306788389883E-5
40	0.6830246571824244	4.792780557647246E-5	1.1785525961427655E-5	2.1135376557493595E-4	7.699876961466068E-5
41	0.6853762624625946	4.8713507307234306E-5	1.2571227692189498E-5	2.1292516903645962E-4	7.93558748069462E-5
42	0.686625528214506	4.8713507307234306E-5	1.3356929422951342E-5	2.2078218634407807E-4	8.092727826846989E-5
43	0.6879140790529553	4.949920903799615E-5	1.3356929422951342E-5	2.2392499326712544E-4	8.328438346075543E-5
44	0.6890517751590985	4.949920903799615E-5	1.3356929422951342E-5	2.2863920365169652E-4	8.485578692227912E-5
45	0.6894076980431336	5.028491076875799E-5	1.3356929422951342E-5	2.302106071132202E-4	9.192710249913571E-5
46	0.689779334961784	5.107061249951984E-5	1.3356929422951342E-5	2.3256771230550571E-4	9.192710249913571E-5
47	0.6902350419656258	6.364184019170933E-5	1.3356929422951342E-5	2.3649622095931494E-4	9.428420769142124E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACTA	197800	0.0	44.17247	1
ACTAAGC	96105	0.0	42.210293	4
CAAGTTA	178155	0.0	41.76961	1
GAGATCG	80785	0.0	39.43919	3
TAAGCAG	150930	0.0	37.66501	6
TAAACTT	175250	0.0	35.618114	1
CTAAGCA	110550	0.0	35.465176	5
AGAGTAA	120550	0.0	35.035835	2
CGTAGTA	19390	0.0	34.891438	1
CTCGTAT	97015	0.0	34.698708	16
GCTCGTA	97305	0.0	34.59257	15
AGATCGG	97800	0.0	34.314518	4
GATCGGA	98280	0.0	34.200855	5
AACTAAG	131410	0.0	34.199337	3
TATGCCG	97905	0.0	34.042233	20
GAGTAAG	73325	0.0	34.011795	3
AAACTAA	160025	0.0	33.72095	2
TCGTATG	100800	0.0	33.40104	17
ATGCCGT	99795	0.0	33.23553	21
TGCCGTC	100820	0.0	32.92918	22
>>END_MODULE