##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104517449
Sequences flagged as poor quality	0
Sequence length	59
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.972100333218044	31.0	31.0	34.0	27.0	34.0
2	31.860917175657434	33.0	31.0	34.0	30.0	34.0
3	31.163243038968545	34.0	31.0	34.0	27.0	34.0
4	34.20589247255738	37.0	35.0	37.0	28.0	37.0
5	34.85223752447307	37.0	35.0	37.0	32.0	37.0
6	35.47851187986802	37.0	35.0	37.0	33.0	37.0
7	35.757901324208554	37.0	35.0	37.0	33.0	37.0
8	35.85465321680402	37.0	35.0	37.0	35.0	37.0
9	37.631637794757125	39.0	37.0	39.0	35.0	39.0
10	37.69937579513637	39.0	37.0	39.0	35.0	39.0
11	37.72074881008625	39.0	38.0	39.0	35.0	39.0
12	37.540816959663836	39.0	37.0	39.0	35.0	39.0
13	37.63418728292919	39.0	37.0	39.0	35.0	39.0
14	38.62846573111443	40.0	39.0	40.0	36.0	40.0
15	38.62387385669928	40.0	39.0	40.0	36.0	40.0
16	38.59982789093905	40.0	39.0	40.0	36.0	40.0
17	38.604016492978126	40.0	39.0	40.0	36.0	40.0
18	38.641581435842355	40.0	39.0	40.0	36.0	40.0
19	38.66349415971681	40.0	39.0	40.0	36.0	40.0
20	38.651718642692856	40.0	39.0	40.0	36.0	40.0
21	38.55795455742514	40.0	39.0	40.0	36.0	40.0
22	38.555065881870114	40.0	39.0	40.0	36.0	40.0
23	38.58584653171166	40.0	39.0	40.0	36.0	40.0
24	38.598856311542775	40.0	39.0	40.0	36.0	40.0
25	38.526159206201065	40.0	39.0	40.0	35.0	40.0
26	38.50303597631817	40.0	39.0	40.0	35.0	40.0
27	38.441954194653185	40.0	39.0	40.0	35.0	40.0
28	38.211352048977005	40.0	38.0	40.0	34.0	40.0
29	38.21728331697035	40.0	38.0	40.0	34.0	40.0
30	38.127039055459534	40.0	38.0	40.0	34.0	40.0
31	37.88511931629713	40.0	38.0	40.0	34.0	40.0
32	37.88287282059477	40.0	38.0	40.0	34.0	40.0
33	37.90395395126799	40.0	38.0	40.0	34.0	40.0
34	37.812051689091646	40.0	38.0	40.0	33.0	40.0
35	37.71106908665557	40.0	38.0	40.0	33.0	40.0
36	37.69063017410615	40.0	38.0	40.0	33.0	40.0
37	37.533444774374466	40.0	38.0	40.0	33.0	40.0
38	37.53589465238479	40.0	38.0	40.0	33.0	40.0
39	37.44031295673893	40.0	37.0	40.0	33.0	40.0
40	37.42043585468681	40.0	37.0	40.0	32.0	40.0
41	37.50832381107962	40.0	38.0	40.0	33.0	40.0
42	37.46204072585048	40.0	38.0	40.0	33.0	40.0
43	37.606113846119605	40.0	38.0	40.0	33.0	40.0
44	37.54210750015531	40.0	38.0	40.0	33.0	40.0
45	37.37476249539921	40.0	38.0	40.0	32.0	40.0
46	37.13480987275149	40.0	38.0	40.0	31.0	40.0
47	37.021491349257865	40.0	37.0	40.0	31.0	40.0
48	36.962266970369704	40.0	37.0	40.0	31.0	40.0
49	37.013205861922636	40.0	37.0	40.0	31.0	40.0
50	36.978819756689624	40.0	37.0	40.0	31.0	40.0
51	36.734897404547254	40.0	37.0	40.0	30.0	40.0
52	36.80728214099447	40.0	37.0	40.0	31.0	40.0
53	36.75687745689239	40.0	37.0	40.0	31.0	40.0
54	36.82669546402725	40.0	37.0	40.0	31.0	40.0
55	36.640525105047296	40.0	37.0	40.0	30.0	40.0
56	36.632392596952876	40.0	36.0	40.0	31.0	40.0
57	36.54074982254877	40.0	36.0	40.0	30.0	40.0
58	36.1367983541198	40.0	36.0	40.0	28.0	40.0
59	35.790043670124405	39.0	35.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	18.0
8	160.0
9	310.0
10	414.0
11	518.0
12	772.0
13	1160.0
14	1705.0
15	2967.0
16	5639.0
17	10113.0
18	16317.0
19	25080.0
20	38410.0
21	62842.0
22	105552.0
23	173534.0
24	272238.0
25	403362.0
26	573513.0
27	784212.0
28	1046731.0
29	1392058.0
30	1851842.0
31	2404722.0
32	2683025.0
33	2741394.0
34	3381719.0
35	4587657.0
36	6826421.0
37	1.1803893E7
38	2.9409214E7
39	3.3909936E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.39482468616317	28.657679924813316	24.867975872621997	27.07951951640151
2	21.257160610569436	30.82217783558801	25.863544564697516	22.057116989145037
3	20.845600623107437	27.65643179829236	28.80923069601517	22.68873688258503
4	25.973663019655213	26.24776461966652	26.286243362101192	21.492328998577072
5	23.74979320438638	29.55612416449238	25.51626953696507	21.17781309415617
6	24.271393191006794	25.434957755235683	29.57150628504146	20.722142768716065
7	22.58907218449237	29.631214975405683	27.134696905968305	20.64501593413364
8	22.066002586802515	27.243153437470525	29.290734985313314	21.40010899041365
9	21.89286020557199	25.843741172825602	27.99232021057077	24.271078411031635
10	24.261001624714357	26.99754277393433	26.108931342172347	22.63252425917896
11	24.69574912797575	27.133807102391106	26.289554770897634	21.88088899873551
12	22.44245456086476	29.46187578688416	26.15815757233034	21.93751207992074
13	21.8290622458648	29.88837203632859	26.31873171722743	21.963834000579176
14	24.920068609787826	27.237551502046326	26.038417757402403	21.80396213076345
15	22.377931363403253	29.17521647509786	26.101035053008232	22.34581710849066
16	24.587948946208975	26.89431120730855	26.277597915731754	22.24014193075072
17	22.74395541360754	26.45904226001536	26.115841193177225	24.681161133199875
18	21.705389116414427	26.466364482355477	29.286188376067233	22.542058025162863
19	22.2583943854198	26.65495978571004	26.286954248184912	24.799691580685252
20	24.781682147638335	26.68739743160015	26.41563132678449	22.115289093977026
21	22.377789760253332	26.9596486228821	28.877401131365154	21.78516048549941
22	21.850896877515638	28.921780323972506	26.805715474360646	22.421607324151203
23	22.25148740474904	26.802112248262013	29.028815083307286	21.917585263681666
24	24.670828887145916	26.263975309998237	26.51631212315563	22.548883679700218
25	22.349671010435777	26.508141238694026	26.2603960033506	24.881791747519593
26	22.642290092633242	25.993263574582652	26.518066854080985	24.84637947870312
27	25.179267435048093	26.156967340448578	26.501832244298267	22.16193298020506
28	22.992356041908373	25.960620221413937	29.10318161324431	21.94384212343338
29	22.266665731575596	26.063791511023197	27.013696057583648	24.65584669981756
30	22.169959391182616	26.193496169237733	29.251782637748843	22.384761801830813
31	22.69278309691619	26.028329489748646	29.08845871276479	22.190428700570372
32	22.207193365387248	26.30596064394951	26.787247744632577	24.69959824603067
33	22.31165343501639	26.01242879550189	29.25569681671048	22.420220952771245
34	25.03560816912016	26.171667278255136	26.685506838193113	22.107217714431588
35	22.786099572713454	26.137159164686462	26.455325177329957	24.621416085270127
36	22.368293738206336	26.1670020285321	29.119486067823946	22.34521816543762
37	22.65347100080868	26.363031497257456	29.095690997969154	21.88780650396471
38	25.033261192588043	26.554646392106257	26.584297900343895	21.827794514961806
39	22.58794988385145	29.28658065506363	26.1576112520695	21.967858209015414
40	22.172337941390055	29.706066591808987	26.13351862424426	21.98807684255669
41	22.126558025732145	29.67182254897936	26.278636976683195	21.922982448605303
42	22.420216168881044	29.47184445728292	26.164141262192498	21.943798111643538
43	22.171032896143494	29.624307994734927	26.14262715118506	22.06203195793652
44	22.275009792862434	29.535982073194305	26.234483583693287	21.954524550249978
45	22.20163257141877	29.25726210558392	26.3436289953843	22.197476327613007
46	22.19345690306697	29.142110041357782	26.36504838536578	22.29938467020947
47	22.38852385308409	28.91614872842907	26.474365060325955	22.220962358160882
48	22.385561668272253	28.747861995751546	26.446659638621682	22.419916697354523
49	22.523851495839704	28.57197940221446	26.480745813074712	22.423423288871124
50	22.685159489493472	28.224038456966166	26.676210782756478	22.414591270783887
51	23.020641271104886	28.081608650819632	26.484739404613673	22.413010673461805
52	22.727341919720985	28.073816650461875	26.589188949684374	22.609652480132766
53	22.855794155481156	27.95860813633138	26.588015939807335	22.597581768380127
54	22.664613637862516	27.891114143055674	26.64425918011068	22.80001303897113
55	22.69969103436499	27.820487658477006	26.679758515728796	22.80006279142921
56	22.853773440260678	27.697481403320513	26.717146531197866	22.731598625220943
57	22.832757810612083	27.692894609396753	26.62661427949701	22.84773330049416
58	23.179289421807454	27.60893063894049	26.51628246303639	22.69549747621567
59	22.970439127346097	27.55608587423522	26.660788477529717	22.812686520888967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5847.0
1	4089.0
2	2331.0
3	2230.0
4	2325.5
5	2522.0
6	2824.0
7	3126.0
8	3656.0
9	5407.0
10	6628.0
11	9671.5
12	12715.0
13	17293.0
14	31568.0
15	41265.0
16	62355.5
17	83446.0
18	130705.5
19	275828.5
20	373692.0
21	429700.0
22	485708.0
23	600765.5
24	715823.0
25	840218.0
26	1125812.0
27	1287011.0
28	1501278.0
29	1715545.0
30	1937761.5
31	2383955.0
32	2607932.0
33	2923889.0
34	3239846.0
35	3590662.0
36	4312854.5
37	4684231.0
38	5127006.5
39	5569782.0
40	5987367.0
41	6404952.0
42	6673268.5
43	7116568.5
44	7291552.0
45	7320089.5
46	7348627.0
47	7329787.5
48	7190570.5
49	7070193.0
50	6879069.5
51	6687946.0
52	6374732.0
53	5699005.0
54	5336492.0
55	4931572.0
56	4526652.0
57	4012231.0
58	3021569.0
59	2545328.0
60	2211430.0
61	1877532.0
62	1610576.5
63	1343621.0
64	1114452.0
65	729526.5
66	573770.0
67	450242.5
68	326715.0
69	251869.5
70	143500.0
71	109976.0
72	76545.5
73	43115.0
74	32524.0
75	16302.0
76	10671.0
77	8367.0
78	6063.0
79	4656.5
80	3250.0
81	2722.0
82	1810.5
83	1427.0
84	972.0
85	517.0
86	419.5
87	258.0
88	194.0
89	161.5
90	129.0
91	128.0
92	100.0
93	73.0
94	83.5
95	94.0
96	92.0
97	65.0
98	40.0
99	28.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.04517449E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.26318077752258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.05940639729405	22.86734653908338
2	17.28758662392533	12.53805757900971
3	7.072549929863317	7.694194699940643
4	3.5727407613645292	5.182357764023423
5	2.121783493750796	3.8471309202324284
6	1.4258496738270356	3.102350669013684
7	0.9811766132299166	2.4906409430163667
8	0.732458671821216	2.1249024982653473
9	0.5391934844440315	1.75975837204106
>10	2.8781686003304823	18.473735472183844
>50	0.1881588178042434	4.727458337744239
>100	0.12437925267356553	9.241218206731899
>500	0.011696544111269316	2.8497863396143974
>1k	0.004795037913373844	2.878277778600915
>5k	4.008270356705836E-5	0.10550519319541395
>10k+	1.6014942874445705E-5	0.11727868730345928
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0026263557197994756	0.0	0.0	1.913556079999618E-6	1.913556079999618E-6
2	0.1297859843479341	0.0	0.0	4.7838901999990455E-6	1.913556079999618E-6
3	0.16448162641244718	9.56778039999809E-7	9.56778039999809E-7	1.1481336479997708E-5	2.870334119999427E-6
4	0.31381458611757734	1.913556079999618E-6	9.56778039999809E-7	1.3394892559997326E-5	2.870334119999427E-6
5	3.0691506831553075	1.913556079999618E-6	9.56778039999809E-7	1.6265226679996755E-5	2.870334119999427E-6
6	3.427405695674796	2.870334119999427E-6	9.56778039999809E-7	1.9135560799996182E-5	4.7838901999990455E-6
7	3.429797640774795	2.870334119999427E-6	9.56778039999809E-7	2.009233883999599E-5	4.7838901999990455E-6
8	3.4303477881477953	3.827112159999236E-6	9.56778039999809E-7	2.3919450999995226E-5	5.740668239998854E-6
9	3.430468342180835	4.7838901999990455E-6	9.56778039999809E-7	2.5833007079994843E-5	8.61100235999828E-6
10	3.430617599555075	4.7838901999990455E-6	9.56778039999809E-7	2.7746563159994463E-5	1.05245584399979E-5
11	3.4306931850202353	4.7838901999990455E-6	9.56778039999809E-7	3.1573675319993696E-5	1.4351670599997135E-5
12	3.4307477213685154	5.740668239998854E-6	9.56778039999809E-7	3.6357565519992744E-5	1.6265226679996755E-5
13	3.430853923730955	5.740668239998854E-6	9.56778039999809E-7	4.018467767999198E-5	1.6265226679996755E-5
14	3.430984045544395	5.740668239998854E-6	9.56778039999809E-7	4.114145571999179E-5	1.9135560799996182E-5
15	3.4311438274770754	5.740668239998854E-6	9.56778039999809E-7	4.3055011799991404E-5	2.009233883999599E-5
16	3.431439471891435	5.740668239998854E-6	9.56778039999809E-7	5.070923611998988E-5	2.10491168799958E-5
17	3.432264214561915	6.697446279998663E-6	9.56778039999809E-7	5.1666014159989685E-5	2.2962672959995416E-5
18	3.4336888570634745	8.61100235999828E-6	9.56778039999809E-7	5.453634827998911E-5	2.2962672959995416E-5
19	3.440765187447313	1.05245584399979E-5	9.56778039999809E-7	5.740668239998854E-5	2.5833007079994843E-5
20	3.4445301090347127	1.2438114519997518E-5	9.56778039999809E-7	6.219057259998759E-5	2.7746563159994463E-5
21	3.448354350860592	1.2438114519997518E-5	9.56778039999809E-7	6.41041286799872E-5	3.061689727999389E-5
22	3.4499110287316714	1.2438114519997518E-5	9.56778039999809E-7	6.793124083998645E-5	3.1573675319993696E-5
23	3.4501396986832313	1.2438114519997518E-5	9.56778039999809E-7	6.984479691998606E-5	3.6357565519992744E-5
24	3.452651241038231	1.2438114519997518E-5	9.56778039999809E-7	7.175835299998567E-5	3.8271121599992364E-5
25	3.4529966379106707	1.2438114519997518E-5	9.56778039999809E-7	7.46286871199851E-5	3.8271121599992364E-5
26	3.4533745652364707	1.2438114519997518E-5	9.56778039999809E-7	8.036935535998395E-5	3.8271121599992364E-5
27	3.4544337185267504	1.4351670599997135E-5	9.56778039999809E-7	8.036935535998395E-5	3.8271121599992364E-5
28	3.4550900682621903	1.4351670599997135E-5	9.56778039999809E-7	8.611002359998281E-5	3.8271121599992364E-5
29	3.4564477363009503	1.6265226679996755E-5	9.56778039999809E-7	8.993713575998205E-5	3.922789963999217E-5
30	3.4573595457730697	1.8178782759996372E-5	9.56778039999809E-7	9.185069183998166E-5	3.922789963999217E-5
31	3.4584837599700697	2.200589491999561E-5	9.56778039999809E-7	9.280746987998148E-5	4.018467767999198E-5
32	3.4606221588894694	2.5833007079994843E-5	9.56778039999809E-7	9.472102595998109E-5	4.018467767999198E-5
33	3.4654739803303083	2.5833007079994843E-5	9.56778039999809E-7	1.0046169419997994E-4	4.114145571999179E-5
34	3.466080577607668	2.6789785119994653E-5	9.56778039999809E-7	1.0046169419997994E-4	4.114145571999179E-5
35	3.471082613200787	2.6789785119994653E-5	1.913556079999618E-6	1.0237525027997957E-4	4.3055011799991404E-5
36	3.472347473769667	2.870334119999427E-5	1.913556079999618E-6	1.0428880635997918E-4	4.401178983999122E-5
37	3.4749920082722263	3.061689727999389E-5	1.913556079999618E-6	1.0811591851997842E-4	4.4968567879991024E-5
38	3.4757937882697463	3.1573675319993696E-5	1.913556079999618E-6	1.1194303067997765E-4	4.4968567879991024E-5
39	3.476639580057106	3.1573675319993696E-5	1.913556079999618E-6	1.176836989199765E-4	4.4968567879991024E-5
40	3.477726479910546	3.253045335999351E-5	1.913556079999618E-6	1.2151081107997575E-4	4.4968567879991024E-5
41	3.4791951342019454	3.253045335999351E-5	1.913556079999618E-6	1.2438114519997519E-4	4.592534591999083E-5
42	3.480969957466145	3.348723139999332E-5	1.913556079999618E-6	1.272514793199746E-4	4.975245807999007E-5
43	3.482651016482425	3.444400943999312E-5	1.913556079999618E-6	1.3203536951997364E-4	5.26227921999895E-5
44	3.4841062758812646	3.444400943999312E-5	2.870334119999427E-6	1.358624816799729E-4	5.5493126319988925E-5
45	3.4845176904384645	3.540078747999294E-5	2.870334119999427E-6	1.358624816799729E-4	5.5493126319988925E-5
46	3.4849262346615446	3.8271121599992364E-5	2.870334119999427E-6	1.387328157999723E-4	5.644990435998873E-5
47	3.485445765137264	3.8271121599992364E-5	3.827112159999236E-6	1.4255992795997153E-4	5.836346043998835E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGATCG	325250	0.0	46.126705	4
TGAGATC	326895	0.0	45.03529	3
CTGAGAT	327635	0.0	44.993427	2
GCTGAGA	325730	0.0	44.08504	1
CTCGTAT	372795	0.0	41.122467	17
AGATCGG	373065	0.0	41.06713	5
GATCGGA	372720	0.0	41.04969	6
GCTCGTA	373430	0.0	41.03196	16
TATGCCG	373975	0.0	40.95162	21
ATGCCGT	372975	0.0	40.755898	22
TCGTATG	377130	0.0	40.67437	18
TGCCGTC	374160	0.0	40.526955	23
GCCGTCT	374800	0.0	40.45139	24
CGTATGC	379780	0.0	40.391953	19
GAGCTCG	383465	0.0	40.088108	14
AGCTCGT	384080	0.0	39.929394	15
CCGTCTT	378385	0.0	39.73547	25
GTATGCC	386235	0.0	39.690823	20
ATCGGAA	390340	0.0	39.225895	7
CGTCTTC	386860	0.0	38.61564	26
>>END_MODULE