FastQCFastQC Report
Thu 26 May 2016
SRR805342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123798880
Sequences flagged as poor quality0
Sequence length59
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA1287700.050.332921
CGCTTAG479450.046.837212
TCGCTTC1085600.046.51171
TCGCTTG816650.044.965471
TCGCTTT1061950.044.1387981
CGCTTAA473950.043.315862
CGCTTAT348800.042.7889942
CCGGTTA363600.042.1915551
TCTCTTA2370050.041.690221
CGCTTAC271650.041.6644552
CCGGTTG271100.039.989491
TAGATCG212400.038.6021655
CTCTTAG1406700.038.5791972
GCTCTTA1705050.037.0880361
CGCTTTG549750.036.928882
CGCTTTA269700.036.7875442
CTCTTAT1043300.035.6643832
ACGTTTA322050.035.3333361
CGCTTCA446150.034.640372
GCGCTAA88700.034.297631