##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123798880
Sequences flagged as poor quality	0
Sequence length	59
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.724567088167518	30.0	25.0	31.0	10.0	34.0
2	29.853225279582496	31.0	28.0	33.0	25.0	34.0
3	25.670768265431803	30.0	16.0	31.0	10.0	34.0
4	32.58710442291562	35.0	32.0	37.0	19.0	37.0
5	33.83208740660659	35.0	33.0	37.0	30.0	37.0
6	34.39262729194319	35.0	35.0	37.0	32.0	37.0
7	35.1005492133693	37.0	35.0	37.0	32.0	37.0
8	35.54221429951547	37.0	35.0	37.0	33.0	37.0
9	37.435434738989564	39.0	37.0	39.0	34.0	39.0
10	37.57452272589219	39.0	37.0	39.0	35.0	39.0
11	37.59828832054054	39.0	37.0	39.0	35.0	39.0
12	37.58814735642196	39.0	37.0	39.0	35.0	39.0
13	37.5927860009719	39.0	37.0	39.0	35.0	39.0
14	38.540093771446074	40.0	38.0	40.0	36.0	40.0
15	38.51657149079216	40.0	38.0	40.0	36.0	40.0
16	38.47607169790228	40.0	38.0	40.0	35.0	40.0
17	38.50857320357018	40.0	38.0	40.0	36.0	40.0
18	38.489529872968156	40.0	38.0	40.0	35.0	40.0
19	38.57780340985314	40.0	39.0	40.0	36.0	40.0
20	38.528481243125945	40.0	39.0	40.0	35.0	40.0
21	38.51115884085542	40.0	39.0	40.0	35.0	40.0
22	38.476946826982605	40.0	39.0	40.0	35.0	40.0
23	38.45632197964957	40.0	39.0	40.0	35.0	40.0
24	38.476652567454565	40.0	38.0	40.0	35.0	40.0
25	38.35708512064083	40.0	38.0	40.0	35.0	40.0
26	38.31880381308781	40.0	38.0	40.0	35.0	40.0
27	38.322970773241245	40.0	38.0	40.0	35.0	40.0
28	38.20531918382461	40.0	38.0	40.0	34.0	40.0
29	38.15125026171481	40.0	38.0	40.0	34.0	40.0
30	38.01976024338831	40.0	38.0	40.0	34.0	40.0
31	38.00204645631689	40.0	38.0	40.0	34.0	40.0
32	37.88750705176008	40.0	38.0	40.0	34.0	40.0
33	37.867552783999336	40.0	38.0	40.0	34.0	40.0
34	37.83317692373308	40.0	38.0	40.0	34.0	40.0
35	37.623804956878445	40.0	38.0	40.0	33.0	40.0
36	37.61954782628082	40.0	38.0	40.0	33.0	40.0
37	37.600741274880676	40.0	38.0	40.0	33.0	40.0
38	37.52440863762257	40.0	37.0	40.0	33.0	40.0
39	37.532707412215686	40.0	37.0	40.0	33.0	40.0
40	37.46946367366167	40.0	37.0	40.0	33.0	40.0
41	37.358185469852394	40.0	37.0	40.0	32.0	40.0
42	37.209439729987864	40.0	37.0	40.0	32.0	40.0
43	37.204339780779925	40.0	37.0	40.0	32.0	40.0
44	37.125428154115774	40.0	37.0	40.0	31.0	40.0
45	37.04799169427058	40.0	37.0	40.0	31.0	40.0
46	36.98349594115876	40.0	36.0	40.0	31.0	40.0
47	36.912219359335076	40.0	36.0	40.0	31.0	40.0
48	37.072972970353206	40.0	37.0	40.0	31.0	40.0
49	37.15981407101583	40.0	37.0	40.0	32.0	40.0
50	37.15759044023662	40.0	37.0	40.0	32.0	40.0
51	37.08282671862621	40.0	37.0	40.0	31.0	40.0
52	37.02817951987934	40.0	36.0	40.0	31.0	40.0
53	36.98644469158364	40.0	36.0	40.0	31.0	40.0
54	36.89613829301202	40.0	36.0	40.0	31.0	40.0
55	36.99507169208639	40.0	36.0	40.0	31.0	40.0
56	37.01056510365845	40.0	36.0	40.0	31.0	40.0
57	36.964796345491976	40.0	36.0	40.0	31.0	40.0
58	36.801974282804494	39.0	36.0	40.0	31.0	40.0
59	36.31327053201127	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	207.0
8	1251.0
9	2330.0
10	3228.0
11	4739.0
12	6598.0
13	9029.0
14	11850.0
15	15613.0
16	21194.0
17	29250.0
18	38677.0
19	50559.0
20	68350.0
21	96834.0
22	142004.0
23	212232.0
24	313316.0
25	451807.0
26	634135.0
27	866079.0
28	1157543.0
29	1521724.0
30	1952530.0
31	2485831.0
32	3113365.0
33	3894662.0
34	5083334.0
35	7026399.0
36	1.0685986E7
37	2.0328271E7
38	4.4288764E7
39	1.9281189E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.729538263997217	26.308670966974823	32.83029377971756	24.131496989310406
2	20.684019112289224	23.010166166285188	22.968034928910505	33.337779792515086
3	22.020427810009267	21.643219227831466	36.06946363327358	20.26688932888569
4	20.18667454826732	23.626078846593764	25.8199637993494	30.367282805789518
5	18.755781150847245	25.342191302538442	39.76385731437958	16.13817023223473
6	18.90586005301502	22.741523186639494	40.43205802831172	17.920558732033765
7	23.052285287233616	28.299249557023455	26.419428834897374	22.22903632084555
8	23.344056101315296	26.918909928748953	27.867173757953225	21.86986021198253
9	21.758570029066497	26.223180694364924	28.765807089692576	23.252442186876003
10	22.095960803522615	28.037476591064475	26.614286009695725	23.252276595717184
11	23.173398660795637	27.85128023775336	26.470590040879205	22.504731060571793
12	23.10894492745007	27.24439833381368	27.074495342768852	22.572161395967395
13	22.64045038210362	27.685254503110208	26.950004717328625	22.72429039745755
14	23.082987503602617	27.363615082785884	26.90854392220673	22.644853491404767
15	22.77533528574733	27.074140735360448	27.072148794884086	23.078375184008127
16	22.70996393505337	27.4425406756507	27.05012355523733	22.7973718340586
17	23.10030187672134	27.22207664560455	26.87414619582988	22.80347528184423
18	22.495955536916004	27.105135361483075	27.279837265086726	23.11907183651419
19	22.909833271512632	27.221694574296635	26.78330934819443	23.085162805996305
20	23.065236939138707	27.34108418428341	26.834632914288076	22.759045962289804
21	22.830613653370694	27.43812625768505	26.950810863555468	22.78044922538879
22	22.747090280622896	27.006615084078305	27.055391777373107	23.190902857925693
23	23.057524429946376	27.165890353773797	27.10412969810389	22.672455518175934
24	22.84539084683157	26.92274760482486	26.995567326618787	23.236294221724783
25	23.329017193047306	27.06314305912945	26.67395052362348	22.933889224199767
26	23.413068841979833	26.71457447757201	27.085950212150543	22.786406468297614
27	23.47629639298837	26.857049110622	26.908044725445013	22.758609770944616
28	23.60139526302661	26.630732038932827	27.046114633670353	22.72175806437021
29	23.050341004700527	26.82850281036468	27.333151963894984	22.788004221039802
30	23.08230171387657	26.879025076801987	26.98098076493099	23.05769244439045
31	22.95315838075433	26.810609272070955	27.10301175584141	23.1332205913333
32	23.020470782934385	26.97543871156185	27.207305914237672	22.796784591266093
33	23.089864787145086	26.620025964693706	27.293843853837775	22.996265394323437
34	23.484311005075327	26.778192177505964	26.92520481606942	22.812292001349284
35	23.448801798530003	26.763684776469702	26.93432283070735	22.85319059429294
36	23.25319421306558	26.671487658046665	27.056543645629105	23.018774483258653
37	23.1561561784727	26.902766002406487	27.057954805406965	22.883123013713856
38	23.36327517664134	26.956052429553484	26.97739026395069	22.70328212985449
39	23.237925092698738	27.070790139620005	26.814004294707672	22.877280472973585
40	23.081869561340135	27.251213419701376	26.80092501644603	22.86599200251246
41	23.047643080454364	27.259300730345863	26.911865438524163	22.781190750675613
42	23.28838839252827	27.085281385421254	26.792804587569773	22.8335256344807
43	23.070728103517578	27.21730519694524	26.747239554994355	22.964727144542827
44	23.108419074550593	27.197342980808877	26.865491836436643	22.828746108203887
45	23.14950829926733	27.057495188971014	26.88482157512249	22.90817493663917
46	23.04821416801186	27.167371788823935	26.833266989168237	22.951147053995964
47	23.14313425129533	27.108358330866967	26.919165181462063	22.829342236375645
48	23.08291803609209	27.012218527340476	26.866844029606728	23.038019406960707
49	23.124246358286925	27.056017792729627	26.821333924830338	22.99840192415311
50	23.197212284957665	27.02801269284504	26.905061661300973	22.86971336089632
51	23.164980167833505	26.89101710774766	26.91955129157873	23.024451432840102
52	23.166548841152682	26.993243396063033	26.81086048597532	23.029347276808966
53	23.12066635820938	26.998011613675345	26.876886123687065	23.004435904428213
54	23.115268894193548	26.919439820457182	26.849758253063357	23.115533032285914
55	23.0984722963568	27.059189873123245	26.798789294378107	23.043548536141845
56	23.154134350811574	27.028461808378236	26.837550549730338	22.979853291079856
57	23.108964313732077	26.94344084534529	26.82514736805373	23.1224474728689
58	23.172280718533155	26.9947813744357	26.746039221033342	23.086898685997806
59	23.184078078896995	26.998973657920004	26.808075323460116	23.00887293972288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5670.0
1	4166.0
2	2662.0
3	3315.0
4	4380.5
5	4793.0
6	4551.0
7	4309.0
8	4792.0
9	6805.5
10	8336.0
11	11763.0
12	15190.0
13	21393.0
14	39372.5
15	51149.0
16	78863.5
17	106578.0
18	167770.0
19	313279.5
20	397597.0
21	469793.5
22	541990.0
23	682011.5
24	822033.0
25	986640.0
26	1337870.5
27	1524494.0
28	1779725.5
29	2034957.0
30	2291872.5
31	2816031.0
32	3083274.0
33	3442051.5
34	3800829.0
35	4174941.0
36	4943712.0
37	5338371.0
38	5784206.0
39	6230041.0
40	6667962.0
41	7105883.0
42	7425519.0
43	8012082.5
44	8279010.0
45	8415092.0
46	8551174.0
47	8611727.5
48	8629560.5
49	8586840.0
50	8439741.5
51	8292643.0
52	7942161.0
53	7120531.5
54	6649384.0
55	6101929.0
56	5554474.0
57	4929076.5
58	3753667.0
59	3203655.0
60	2792195.0
61	2380735.0
62	2038260.0
63	1695785.0
64	1404401.0
65	911974.5
66	710932.0
67	555329.0
68	399726.0
69	311763.5
70	176535.0
71	129269.0
72	92344.0
73	55419.0
74	41650.5
75	21553.0
76	15224.0
77	11990.0
78	8756.0
79	7000.0
80	5244.0
81	4202.0
82	2573.5
83	1987.0
84	1459.5
85	932.0
86	806.5
87	603.5
88	526.0
89	500.5
90	475.0
91	466.5
92	463.5
93	469.0
94	456.0
95	443.0
96	469.5
97	402.0
98	308.0
99	222.0
100	136.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.2379888E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.25572647229053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11873238596212	34.801418235453085
2	14.73692232694317	14.222817857047215
3	5.129972644999188	7.426516703022408
4	2.45228287859491	4.733467672886289
5	1.4435290604663702	3.4829271748333848
6	0.920121098061626	2.6640667217667264
7	0.619870170615389	2.093859978108379
8	0.42788338045493785	1.6518258695418002
9	0.3225877682730576	1.4010036398182177
>10	1.64954444048316	13.78343187681427
>50	0.09973233763558595	3.303105631343143
>100	0.07065607193737707	7.013812513730226
>500	0.006373344755567656	2.0477273378459167
>1k	0.0017784863788813969	1.30110967635629
>5k	5.118913576850202E-6	0.01618387390021839
>10k+	8.485525048311219E-6	0.0567252375322363
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.028775704594419593	2.42328525104589E-6	0.0	1.6155235006972599E-6	0.0
2	0.030307220873080597	3.2310470013945198E-6	0.0	5.65433225244041E-6	0.0
3	0.037176426798045344	4.84657050209178E-6	0.0	1.050090275453219E-5	0.0
4	0.05536641365414614	5.65433225244041E-6	0.0	1.211642625522945E-5	4.84657050209178E-6
5	0.24860321838129715	5.65433225244041E-6	0.0	1.3731949755926709E-5	9.69314100418356E-6
6	0.40099878124907107	8.0776175034863E-6	0.0	1.696299675732123E-5	1.211642625522945E-5
7	0.40108925056511013	8.0776175034863E-6	0.0	2.180956725941301E-5	1.3731949755926709E-5
8	0.4011118678941199	8.88537925383493E-6	0.0	2.988718476289931E-5	1.3731949755926709E-5
9	0.40116356464614217	1.130866450488082E-5	0.0	3.311823176429383E-5	1.61552350069726E-5
10	0.40123141663317147	1.4539711506275339E-5	0.0	4.281137276847739E-5	2.180956725941301E-5
11	0.40128553667044486	1.534747325662397E-5	0.0	5.008122852161506E-5	2.7463899511853418E-5
12	0.4012944220496987	1.938628200836712E-5	0.0	5.573556077405547E-5	2.9079423012550678E-5
13	0.40132350147271123	2.019404375871575E-5	0.0	6.219765477684451E-5	4.200361101812876E-5
14	0.4013533886574741	2.019404375871575E-5	0.0	6.704422527893628E-5	4.685018152022054E-5
15	0.40137681374823425	2.100180550906438E-5	8.077617503486299E-7	7.027527228033081E-5	5.1696752022312316E-5
16	0.4014252794532551	2.342509076011027E-5	8.077617503486299E-7	7.754512803346847E-5	5.65433225244041E-5
17	0.40147374515827605	2.665613776150479E-5	8.077617503486299E-7	7.996841328451436E-5	6.381317827754177E-5
18	0.40154805923930814	2.827166126220205E-5	8.077617503486299E-7	8.64305072873034E-5	6.542870177823903E-5
19	0.40192205292971955	2.988718476289931E-5	8.077617503486299E-7	9.046931603904655E-5	7.189079578102807E-5
20	0.4020569491420278	2.988718476289931E-5	8.077617503486299E-7	9.45081247907897E-5	7.512184278242259E-5
21	0.40230008542888274	3.069494651324794E-5	8.077617503486299E-7	1.0177798054392738E-4	8.0776175034863E-5
22	0.4023897469831714	3.392599351464246E-5	8.077617503486299E-7	1.0581678929567052E-4	8.481498378660615E-5
23	0.40245032911444756	3.4733755264991086E-5	8.077617503486299E-7	1.1066335979776231E-4	9.046931603904655E-5
24	0.40272739139481717	3.4733755264991086E-5	8.077617503486299E-7	1.1389440679915682E-4	9.127707778939519E-5
25	0.40282836161361074	3.715704051603698E-5	8.077617503486299E-7	1.1712545380055135E-4	9.45081247907897E-5
26	0.4029624500641686	3.877256401673424E-5	8.077617503486299E-7	1.2277978605299176E-4	9.531588654113833E-5
27	0.4030819988032202	4.03880875174315E-5	1.6155235006972599E-6	1.2843411830543215E-4	9.773917179218423E-5
28	0.40319427768651867	4.03880875174315E-5	1.6155235006972599E-6	1.3408845055787257E-4	9.854693354253286E-5
29	0.4034139888826135	4.1195849267780125E-5	1.6155235006972599E-6	1.3812725930961573E-4	1.0420126579497327E-4
30	0.4035682713769301	4.1195849267780125E-5	1.6155235006972599E-6	1.4701263856345065E-4	1.0824007454671642E-4
31	0.4037879825730249	4.1195849267780125E-5	2.42328525104589E-6	1.5185920906554242E-4	1.1066335979776231E-4
32	0.40419913330395235	4.523465801952328E-5	2.42328525104589E-6	1.5428249431658834E-4	1.1793321555089998E-4
33	0.4049390430672717	4.604241976987191E-5	2.42328525104589E-6	1.5751354131798284E-4	1.2277978605299176E-4
34	0.40527426419366636	4.685018152022054E-5	2.42328525104589E-6	1.5993682656902873E-4	1.2520307130403765E-4
35	0.405852621606916	4.7657943270569165E-5	3.2310470013945198E-6	1.696299675732123E-4	1.2843411830543215E-4
36	0.4061434158370415	5.008122852161506E-5	3.2310470013945198E-6	1.752842998256527E-4	1.373194975592671E-4
37	0.40656425970897314	5.1696752022312316E-5	3.2310470013945198E-6	1.7689982332634997E-4	1.4378159156205615E-4
38	0.40701499076566766	5.412003727335821E-5	4.03880875174315E-6	1.7932310857739586E-4	1.5185920906554242E-4
39	0.40756265323240404	5.4927799023706836E-5	4.84657050209178E-6	1.8416967907948763E-4	1.558980178172856E-4
40	0.4081167777931432	5.65433225244041E-5	4.84657050209178E-6	1.8982401133192805E-4	1.558980178172856E-4
41	0.4088146839454444	6.058213127614725E-5	4.84657050209178E-6	1.9224729658297394E-4	1.6236011182007461E-4
42	0.40977672819010963	6.219765477684451E-5	5.65433225244041E-6	1.970938670850657E-4	1.6639892057181778E-4
43	0.4104043590701305	6.381317827754177E-5	5.65433225244041E-6	2.0517148458855202E-4	1.7447653807530408E-4
44	0.4108857850733383	6.542870177823903E-5	5.65433225244041E-6	2.1163357859134104E-4	1.7770758507669858E-4
45	0.4113405549387846	6.623646352858766E-5	6.4620940027890395E-6	2.1244134034168968E-4	1.8740072608088216E-4
46	0.4118357128917483	6.704422527893628E-5	6.4620940027890395E-6	2.1971119609482734E-4	1.9063177308227666E-4
47	0.4123809520732336	6.865974877963355E-5	6.4620940027890395E-6	2.26981051847965E-4	1.938628200836712E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	128770	0.0	50.33292	1
CGCTTAG	47945	0.0	46.83721	2
TCGCTTC	108560	0.0	46.5117	1
TCGCTTG	81665	0.0	44.96547	1
TCGCTTT	106195	0.0	44.138798	1
CGCTTAA	47395	0.0	43.31586	2
CGCTTAT	34880	0.0	42.788994	2
CCGGTTA	36360	0.0	42.191555	1
TCTCTTA	237005	0.0	41.69022	1
CGCTTAC	27165	0.0	41.664455	2
CCGGTTG	27110	0.0	39.98949	1
TAGATCG	21240	0.0	38.602165	5
CTCTTAG	140670	0.0	38.579197	2
GCTCTTA	170505	0.0	37.088036	1
CGCTTTG	54975	0.0	36.92888	2
CGCTTTA	26970	0.0	36.787544	2
CTCTTAT	104330	0.0	35.664383	2
ACGTTTA	32205	0.0	35.333336	1
CGCTTCA	44615	0.0	34.64037	2
GCGCTAA	8870	0.0	34.29763	1
>>END_MODULE