FastQCFastQC Report
Thu 26 May 2016
SRR805340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121345710
Sequences flagged as poor quality0
Sequence length59
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC216650.040.413575
CCTAAGC780250.040.0429344
CCTAACG196050.036.2524874
TAGCCTA534500.036.103461
TAACGCA331600.036.041926
CTAAGCA1093600.034.314795
CTTGCGT197250.033.130045
AGTAATC590750.032.903511
TCGTTGA478250.031.7279646
GTAATCG122750.030.871692
TTGCGTT301400.030.5532516
TCGCAGA405850.030.4079076
ATCGTTG321200.029.181355
GCCTAAG391300.028.3217473
CTCGTTG217200.028.2325045
CCTTGCG190600.028.154514
TACCCTA507350.028.1374781
TACCCTT402350.028.1301081
ATTGCGT112800.025.5132985
ATCGCAG256050.024.6422565