##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR805340_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 121345710 Sequences flagged as poor quality 0 Sequence length 59 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.246952232592317 31.0 28.0 33.0 16.0 34.0 2 27.85101296123283 31.0 25.0 34.0 16.0 34.0 3 26.391322659861647 30.0 16.0 33.0 16.0 34.0 4 31.809407262934965 35.0 30.0 37.0 19.0 37.0 5 33.341029691119694 35.0 33.0 37.0 28.0 37.0 6 34.02349578736653 35.0 35.0 37.0 29.0 37.0 7 34.80530478580578 36.0 35.0 37.0 32.0 37.0 8 35.16620439239261 37.0 35.0 37.0 32.0 37.0 9 36.97852788532862 39.0 37.0 39.0 33.0 39.0 10 37.10342780144432 39.0 37.0 39.0 33.0 39.0 11 37.13356214241113 39.0 37.0 39.0 34.0 39.0 12 37.20396384841293 39.0 37.0 39.0 34.0 39.0 13 37.05248814317375 39.0 37.0 39.0 33.0 39.0 14 38.00504272462537 40.0 38.0 40.0 34.0 40.0 15 38.12845348220386 40.0 38.0 40.0 34.0 40.0 16 38.042667589979075 40.0 38.0 40.0 34.0 40.0 17 38.12424208486645 40.0 38.0 40.0 34.0 40.0 18 38.130726047093056 40.0 38.0 40.0 34.0 40.0 19 38.20158500040916 40.0 38.0 40.0 34.0 40.0 20 38.144566725927106 40.0 38.0 40.0 34.0 40.0 21 38.09223587714803 40.0 38.0 40.0 34.0 40.0 22 38.15878678364484 40.0 38.0 40.0 34.0 40.0 23 38.18398152682942 40.0 38.0 40.0 34.0 40.0 24 38.06345688693898 40.0 38.0 40.0 34.0 40.0 25 38.12464790885479 40.0 38.0 40.0 34.0 40.0 26 37.888593070162926 40.0 38.0 40.0 34.0 40.0 27 37.86058734997719 40.0 38.0 40.0 34.0 40.0 28 37.68503754273637 40.0 38.0 40.0 33.0 40.0 29 37.67671242765813 40.0 38.0 40.0 33.0 40.0 30 37.67324609992393 40.0 38.0 40.0 33.0 40.0 31 37.38224449797195 40.0 37.0 40.0 32.0 40.0 32 37.112197069018755 40.0 37.0 40.0 31.0 40.0 33 37.381325413152226 40.0 37.0 40.0 32.0 40.0 34 37.38021883921566 40.0 37.0 40.0 32.0 40.0 35 36.42321197016359 40.0 36.0 40.0 29.0 40.0 36 37.00260582759786 40.0 37.0 40.0 30.0 40.0 37 37.26570978899872 40.0 37.0 40.0 31.0 40.0 38 37.20493591409206 40.0 37.0 40.0 31.0 40.0 39 37.347694516765365 40.0 37.0 40.0 32.0 40.0 40 37.3696151598602 40.0 37.0 40.0 32.0 40.0 41 37.347440886043685 40.0 37.0 40.0 32.0 40.0 42 37.14984799215399 40.0 37.0 40.0 31.0 40.0 43 37.283911165874756 40.0 37.0 40.0 32.0 40.0 44 37.222540302413655 40.0 37.0 40.0 31.0 40.0 45 37.20511684343847 40.0 37.0 40.0 31.0 40.0 46 37.1478897688266 40.0 37.0 40.0 31.0 40.0 47 37.153461296654 40.0 37.0 40.0 31.0 40.0 48 36.998925458510236 40.0 37.0 40.0 31.0 40.0 49 36.913166093799276 40.0 37.0 40.0 31.0 40.0 50 36.98723709309542 40.0 37.0 40.0 31.0 40.0 51 36.93995123519406 40.0 36.0 40.0 31.0 40.0 52 36.96506347855231 40.0 36.0 40.0 31.0 40.0 53 36.99467714186188 40.0 36.0 40.0 31.0 40.0 54 36.8800136650896 40.0 36.0 40.0 31.0 40.0 55 36.79201554797446 40.0 36.0 40.0 31.0 40.0 56 36.75603061698679 40.0 36.0 40.0 31.0 40.0 57 36.66422505583428 39.0 36.0 40.0 31.0 40.0 58 36.25273580747107 39.0 35.0 40.0 29.0 40.0 59 35.89150016922724 38.0 35.0 40.0 28.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 28.0 8 400.0 9 1041.0 10 1716.0 11 3163.0 12 5491.0 13 8665.0 14 12255.0 15 17204.0 16 24766.0 17 36442.0 18 52862.0 19 73773.0 20 104302.0 21 147254.0 22 210447.0 23 303582.0 24 431336.0 25 603641.0 26 827739.0 27 1109136.0 28 1462961.0 29 1886506.0 30 2384665.0 31 2970759.0 32 3602584.0 33 4406171.0 34 5562430.0 35 7360038.0 36 1.0809608E7 37 1.9621751E7 38 3.5065502E7 39 2.2237492E7 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.754806577010427 30.62602789995625 26.122324390371936 26.496841132661388 2 24.639961313836313 29.711572003657977 25.370524429747043 20.277942252758667 3 22.096635307502837 27.29568354744473 27.20153106360332 23.40615008144911 4 21.23631399906927 26.066649575003513 24.920121197527294 27.77691522839992 5 21.755572570303475 29.275421438467003 25.012060170895207 23.956945820334315 6 22.167878040352644 24.59630175636205 32.70645332249488 20.529366880790427 7 22.566886789817293 27.786063471053073 27.347007158308273 22.30004258082136 8 22.720634293540332 27.569474025904995 27.715070438007245 21.99482124254743 9 21.879974166371436 26.199130566708952 28.653707658886336 23.26718760803328 10 22.256080581670336 27.286086174781126 27.12444139970008 23.333391843848457 11 23.229843065733434 27.654817792899312 26.54363471110763 22.57170443025963 12 22.714217090987397 27.421736623404318 27.07917980783993 22.784866477768354 13 22.84720654731016 27.285263731202363 27.187003974017703 22.680525747469773 14 23.419862144281822 27.420043114832822 27.007015740399886 22.153079000485473 15 23.050568495581754 26.954011806433044 26.69950425111856 23.29591544686664 16 22.914428536451762 27.268127567097345 27.15684633597677 22.66059756047412 17 23.262144166448078 27.55841965900566 26.660828800622618 22.518607373923643 18 22.1486008858492 26.7061596161908 27.6321593898952 23.513080108064802 19 22.863394181796785 27.037074487429347 26.926146791674793 23.173384539099075 20 23.55015929281719 27.15845249082147 26.545846573397608 22.745541642963726 21 22.298148817951617 27.756317878893288 26.967593662767314 22.97793964038778 22 22.949794434430355 26.875915102396284 26.97890597038824 23.195384492785116 23 23.264501892979983 26.909596556812765 27.21228216473413 22.613619385473125 24 22.519098532613967 26.83624085268445 27.151967712744028 23.492692901957557 25 23.17536400751209 27.103819327440583 26.79445527987763 22.926361385169695 26 23.549156373142488 26.716483837788747 27.16222518290923 22.57213460615954 27 23.105518110199363 27.009118822577243 26.724015212404296 23.161347854819095 28 23.77304727130444 26.515184591198153 27.46361861494733 22.248149522550076 29 22.873233013346745 26.634296342243992 27.362525630283923 23.129945014125344 30 22.36405226027356 27.11684574592707 26.972848071843657 23.54625392195571 31 23.151753778522536 27.175722981883744 27.155287154362522 22.517236085231197 32 23.068346627169596 26.564759479342122 26.867607433340662 23.499286460147623 33 22.825681270479194 26.536088502840354 27.307386474560985 23.330843752119463 34 23.80035107957257 27.045429953807183 26.573615169419668 22.580603797200578 35 23.087440833301812 26.63206222947643 26.915910747895417 23.364586189326346 36 23.124539796256496 26.495361063856315 27.511169533723113 22.868929606164073 37 23.504353800393933 26.95638189434138 27.10387536568042 22.435388939584268 38 23.553424344379376 26.60175543082652 26.98902334495385 22.855796879840252 39 22.93006320536589 27.25282912762223 27.107598612262436 22.709509054749443 40 23.116506549757712 27.865525695139944 26.588132369904137 22.429835385198206 41 22.529131849819827 26.91000613041862 27.26071650987909 23.300145509882466 42 22.893349917355955 27.132135944484563 27.01081315524051 22.963700982918965 43 23.272379386135693 27.4389436594009 26.38295824384727 22.905718710616142 44 22.573262787782113 27.703734231725207 26.64466259252181 23.078340387970865 45 23.350771939115113 26.906821015757377 26.712162300587305 23.030244744540205 46 23.312509358592077 26.934077850795056 26.846835376380422 22.906577414232444 47 22.83500422058596 27.08063020934156 26.817735048070507 23.26663052200197 48 23.283376066611666 27.17966955733334 26.738150034311058 22.79880434174393 49 23.32005309458406 27.098852526389273 26.850498464263794 22.730595914762873 50 22.871598839382127 27.38762499308793 26.56551269921285 23.17526346831709 51 23.566485374719882 26.887354320148603 26.963802016569023 22.58235828856249 52 22.973467294393842 26.936713296250854 26.92987663099091 23.159942778364393 53 22.490544577142447 27.296547195611616 26.60087035627382 23.612037870972117 54 23.41583563193128 27.251536127647196 26.525779938985895 22.806848301435625 55 23.096064953594155 26.68309988049845 26.74148018912247 23.479354976784922 56 23.000691989852793 26.83041040346626 26.858522645753197 23.31037496092775 57 23.537572939331767 27.0676293376997 26.544803273226552 22.84999444974198 58 23.18535199967102 26.79212804474093 26.96246698791412 23.06005296767393 59 23.282017963387418 26.691134775180764 27.105160124737825 22.921687136693997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3506.0 1 3767.5 2 4029.0 3 4314.5 4 6115.5 5 7631.0 6 6937.5 7 6244.0 8 6094.0 9 6982.5 10 8021.0 11 10340.0 12 12659.0 13 17431.0 14 31441.5 15 40680.0 16 61391.5 17 82103.0 18 127593.5 19 262025.0 20 350966.0 21 418810.5 22 486655.0 23 590265.0 24 693875.0 25 828114.0 26 1136794.5 27 1311236.0 28 1546044.5 29 1780853.0 30 2035255.5 31 2551096.0 32 2812534.0 33 3143810.0 34 3475086.0 35 3816484.5 36 4503992.0 37 4850101.0 38 5287779.0 39 5725457.0 40 6201079.5 41 6676702.0 42 7145517.0 43 8155883.0 44 8697434.0 45 9119360.0 46 9541286.0 47 9851708.5 48 9811699.0 49 9461267.0 50 8877953.5 51 8294640.0 52 7681892.5 53 6567239.0 54 6065333.0 55 5586891.5 56 5108450.0 57 4555375.5 58 3485098.5 59 2967896.0 60 2584570.5 61 2201245.0 62 1893916.5 63 1586588.0 64 1322694.0 65 872576.0 66 686352.0 67 543846.0 68 401340.0 69 313174.0 70 181473.5 71 137939.0 72 97619.0 73 57299.0 74 43036.5 75 21730.0 76 14686.0 77 11392.0 78 8098.0 79 6296.5 80 4495.0 81 3660.0 82 2247.5 83 1670.0 84 1182.0 85 694.0 86 554.0 87 378.0 88 342.0 89 309.0 90 276.0 91 240.5 92 181.5 93 158.0 94 138.0 95 118.0 96 91.0 97 50.0 98 36.0 99 21.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 59 1.2134571E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.303861720499576 #Duplication Level Percentage of deduplicated Percentage of total 1 72.00630224927261 31.90157257856271 2 13.845716510755746 12.268374194275209 3 5.174273278592472 6.877208635165105 4 2.5693436402132037 4.5532738139380315 5 1.5114755120636032 3.348210104019358 6 1.0131192426875686 2.6931056900644377 7 0.686642552509758 2.1294641688462232 8 0.5329827218174835 1.8890554245453828 9 0.37669544697896956 1.5020156694328242 >10 2.068814127993659 16.38721230674042 >50 0.11885303872180825 3.611760037688095 >100 0.08527445920995595 7.5251530562675955 >500 0.006523708803525276 1.974006829365494 >1k 0.003870254281023759 2.9012074098817333 >5k 9.413796910499308E-5 0.27453032700664803 >10k+ 1.9118129580247163E-5 0.16384975420070644 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05141014049858046 1.6481835245761881E-6 0.0 3.2963670491523763E-6 8.240917622880941E-7 2 0.05869263940192035 1.6481835245761881E-6 0.0 6.5927340983047525E-6 1.0713192909745223E-5 3 0.061581905120502406 2.4722752868642823E-6 8.240917622880941E-7 6.5927340983047525E-6 1.0713192909745223E-5 4 0.08516741135718765 4.12045881144047E-6 8.240917622880941E-7 8.24091762288094E-6 1.4009559958897599E-5 5 0.37155990104635755 4.944550573728565E-6 8.240917622880941E-7 1.2361376434321412E-5 1.4009559958897599E-5 6 0.4694290387356916 4.944550573728565E-6 8.240917622880941E-7 1.4833651721185694E-5 1.8130018770338068E-5 7 0.46967544217261575 4.944550573728565E-6 1.6481835245761881E-6 2.0602294057202352E-5 1.977820229491426E-5 8 0.47007183031027633 7.416825860592847E-6 3.2963670491523763E-6 2.225047758177854E-5 2.0602294057202352E-5 9 0.470125396274825 8.24091762288094E-6 3.2963670491523763E-6 2.3074569344066633E-5 2.0602294057202352E-5 10 0.47019297179933267 1.0713192909745223E-5 3.2963670491523763E-6 2.637093639321901E-5 2.1426385819490445E-5 11 0.4703050482790038 1.4009559958897599E-5 3.2963670491523763E-6 2.8019119917795197E-5 2.5546844630930917E-5 12 0.4703305951236348 1.4833651721185694E-5 3.2963670491523763E-6 3.296367049152376E-5 2.637093639321901E-5 13 0.47035202150945427 1.648183524576188E-5 3.2963670491523763E-6 3.543594577838804E-5 2.7195028155507104E-5 14 0.4703948742810932 1.648183524576188E-5 3.2963670491523763E-6 4.1204588114404704E-5 2.8019119917795197E-5 15 0.47050612666900216 1.648183524576188E-5 4.12045881144047E-6 4.532504692584517E-5 3.049139520465948E-5 16 0.4708299947315814 1.648183524576188E-5 4.12045881144047E-6 5.439005631101421E-5 3.296367049152376E-5 17 0.4714843235908381 1.8130018770338068E-5 4.12045881144047E-6 5.851051512245468E-5 3.543594577838804E-5 18 0.4724386218515677 1.8130018770338068E-5 4.12045881144047E-6 6.427915745847134E-5 3.543594577838804E-5 19 0.4746702623438439 1.8954110532626165E-5 5.768642336016658E-6 6.510324922075943E-5 3.543594577838804E-5 20 0.4765467192865739 1.977820229491426E-5 5.768642336016658E-6 6.92237080321999E-5 3.6260037540676136E-5 21 0.47967579570798174 2.1426385819490445E-5 5.768642336016658E-6 7.334416684364038E-5 3.7084129302964236E-5 22 0.48232030617316424 2.1426385819490445E-5 5.768642336016658E-6 7.993690094194513E-5 3.790822106525233E-5 23 0.4862751225403848 2.3074569344066633E-5 5.768642336016658E-6 8.488145151567369E-5 4.038049635211661E-5 24 0.49167292358337183 2.389866110635473E-5 6.5927340983047525E-6 8.982600208940225E-5 4.038049635211661E-5 25 0.49679547797775464 2.389866110635473E-5 6.5927340983047525E-6 9.559464442541892E-5 4.038049635211661E-5 26 0.5050100246642424 2.4722752868642823E-5 7.416825860592847E-6 1.0960420438431651E-4 4.1204588114404704E-5 27 0.5143593457074008 2.4722752868642823E-5 7.416825860592847E-6 1.1372466319575698E-4 4.1204588114404704E-5 28 0.5241248330905147 2.8019119917795197E-5 9.88910114745713E-6 1.1702103024490936E-4 4.1204588114404704E-5 29 0.5389329379670694 2.8019119917795197E-5 1.0713192909745223E-5 1.1949330553177364E-4 4.285277163898089E-5 30 0.5523796432523242 2.9667303442371388E-5 1.0713192909745223E-5 1.2114148905634983E-4 4.532504692584517E-5 31 0.5678915224938731 3.049139520465948E-5 1.1537284672033316E-5 1.252619478677903E-4 4.6973230450421365E-5 32 0.5863791970890442 3.1315486966947575E-5 1.1537284672033316E-5 1.3350286549067123E-4 5.109368926186183E-5 33 0.6059760991962551 3.213957872923567E-5 1.1537284672033316E-5 1.3762332430211172E-4 5.439005631101421E-5 34 0.6284490815538514 3.296367049152376E-5 1.2361376434321412E-5 1.4009559958897598E-4 5.439005631101421E-5 35 0.6525067923703277 3.3787762253811855E-5 1.2361376434321412E-5 1.4174378311355217E-4 5.686233159787849E-5 36 0.6752426600000939 3.543594577838804E-5 1.2361376434321412E-5 1.4256787487584028E-4 5.768642336016659E-5 37 0.7012814874131108 3.543594577838804E-5 1.4009559958897599E-5 1.4586424192499265E-4 5.768642336016659E-5 38 0.7320415365322762 3.543594577838804E-5 1.4833651721185694E-5 1.508087924987212E-4 5.768642336016659E-5 39 0.761441010151904 3.6260037540676136E-5 1.4833651721185694E-5 1.5740152659702596E-4 5.851051512245468E-5 40 0.7915739254399682 3.6260037540676136E-5 1.4833651721185694E-5 1.6317016893304263E-4 5.9334606884742775E-5 41 0.8243703053037474 3.6260037540676136E-5 1.5657743483473787E-5 1.7058699479363548E-4 6.180688217160706E-5 42 0.85521935633324 3.6260037540676136E-5 1.5657743483473787E-5 1.7717972889194023E-4 6.345506569618324E-5 43 0.8895188795714327 3.6260037540676136E-5 1.5657743483473787E-5 1.821242794656688E-4 6.427915745847134E-5 44 0.926917811927591 3.955640458982852E-5 1.5657743483473787E-5 1.8706883003939735E-4 6.757552450762371E-5 45 0.9578921249049513 3.955640458982852E-5 1.648183524576188E-5 1.9036519708854973E-4 7.087189155677608E-5 46 0.9983162981204692 4.1204588114404704E-5 1.7305927008049974E-5 1.936615641377021E-4 7.252007508135227E-5 47 1.0374515918197684 4.532504692584517E-5 1.8130018770338068E-5 1.9943020647371877E-4 7.416825860592847E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAACGC 21665 0.0 40.41357 5 CCTAAGC 78025 0.0 40.042934 4 CCTAACG 19605 0.0 36.252487 4 TAGCCTA 53450 0.0 36.10346 1 TAACGCA 33160 0.0 36.04192 6 CTAAGCA 109360 0.0 34.31479 5 CTTGCGT 19725 0.0 33.13004 5 AGTAATC 59075 0.0 32.90351 1 TCGTTGA 47825 0.0 31.727964 6 GTAATCG 12275 0.0 30.87169 2 TTGCGTT 30140 0.0 30.553251 6 TCGCAGA 40585 0.0 30.407907 6 ATCGTTG 32120 0.0 29.18135 5 GCCTAAG 39130 0.0 28.321747 3 CTCGTTG 21720 0.0 28.232504 5 CCTTGCG 19060 0.0 28.15451 4 TACCCTA 50735 0.0 28.137478 1 TACCCTT 40235 0.0 28.130108 1 ATTGCGT 11280 0.0 25.513298 5 ATCGCAG 25605 0.0 24.642256 5 >>END_MODULE