FastQCFastQC Report
Thu 26 May 2016
SRR805339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134169143
Sequences flagged as poor quality0
Sequence length59
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA346500.045.2679671
TACGTTA339650.042.5529251
CGGTAAG234350.040.1090243
CGTTAAG276500.039.984813
ACGGTAA260900.037.6627042
ACGTTAA329550.037.3275682
TCGCTTC388300.034.996651
GACGGTA312650.033.4375531
CGCTTAG166200.033.1170272
TACGTTT274250.032.1671831
CGCTTAA152400.031.455712
GGTAAGC569200.029.8520744
TCGCTTG315000.029.175241
GTTAAGC426800.028.9090924
TCGCTTT417050.028.2759881
CGCTTAT138800.027.607352
TAAGCAG1204750.026.7496576
CGCTTAC117400.026.0485522
TCGCGTC62350.024.6936651
CGCGTAG39600.024.1578272
Produced by FastQC (version 0.11.5)