##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	134169143
Sequences flagged as poor quality	0
Sequence length	59
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.022905117609643	33.0	31.0	34.0	27.0	34.0
2	32.07341610581801	34.0	31.0	34.0	30.0	34.0
3	31.186948462508997	34.0	31.0	34.0	26.0	34.0
4	35.51749591185806	37.0	35.0	37.0	33.0	37.0
5	34.951227720072715	37.0	35.0	37.0	32.0	37.0
6	35.4998518027353	37.0	35.0	37.0	33.0	37.0
7	35.709989673258924	37.0	35.0	37.0	33.0	37.0
8	35.70702636894684	37.0	35.0	37.0	35.0	37.0
9	37.584818135120685	39.0	37.0	39.0	35.0	39.0
10	37.664184424283015	39.0	37.0	39.0	35.0	39.0
11	37.64592326567965	39.0	37.0	39.0	35.0	39.0
12	37.504432103289204	39.0	37.0	39.0	35.0	39.0
13	37.64453885644928	39.0	37.0	39.0	35.0	39.0
14	38.50505604705249	40.0	39.0	40.0	36.0	40.0
15	38.54879480000852	40.0	39.0	40.0	36.0	40.0
16	38.719696010877854	40.0	39.0	40.0	36.0	40.0
17	38.67244931273057	40.0	39.0	40.0	36.0	40.0
18	38.58022565590957	40.0	39.0	40.0	36.0	40.0
19	38.65979179728382	40.0	39.0	40.0	36.0	40.0
20	38.46685159194913	40.0	39.0	40.0	35.0	40.0
21	38.30598745048256	40.0	39.0	40.0	34.0	40.0
22	38.441141895048105	40.0	39.0	40.0	35.0	40.0
23	38.20667124630885	40.0	38.0	40.0	34.0	40.0
24	38.29880556813276	40.0	38.0	40.0	34.0	40.0
25	38.372620364728725	40.0	38.0	40.0	35.0	40.0
26	38.102725259264716	40.0	38.0	40.0	34.0	40.0
27	38.265097564199245	40.0	38.0	40.0	34.0	40.0
28	37.95109637839753	40.0	38.0	40.0	34.0	40.0
29	38.12459929031521	40.0	38.0	40.0	34.0	40.0
30	38.01333123965769	40.0	38.0	40.0	34.0	40.0
31	38.09704580881164	40.0	38.0	40.0	34.0	40.0
32	38.10157957854736	40.0	38.0	40.0	34.0	40.0
33	37.75254454744486	40.0	38.0	40.0	34.0	40.0
34	37.78517198250271	40.0	38.0	40.0	33.0	40.0
35	37.549103157050055	40.0	38.0	40.0	33.0	40.0
36	37.54910842651801	40.0	38.0	40.0	33.0	40.0
37	37.8315690217981	40.0	38.0	40.0	33.0	40.0
38	37.803769686447204	40.0	38.0	40.0	33.0	40.0
39	37.80663629937623	40.0	38.0	40.0	33.0	40.0
40	37.478582210218036	40.0	38.0	40.0	33.0	40.0
41	37.76756516958598	40.0	38.0	40.0	33.0	40.0
42	37.79578932690954	40.0	38.0	40.0	33.0	40.0
43	37.82368275244927	40.0	38.0	40.0	33.0	40.0
44	37.812799639034736	40.0	38.0	40.0	33.0	40.0
45	37.72343696046415	40.0	38.0	40.0	33.0	40.0
46	37.7894252704588	40.0	38.0	40.0	33.0	40.0
47	37.83171120799363	40.0	38.0	40.0	33.0	40.0
48	37.70372008711422	40.0	38.0	40.0	33.0	40.0
49	37.06403017719208	40.0	37.0	40.0	31.0	40.0
50	37.35565106799557	40.0	37.0	40.0	32.0	40.0
51	37.32650036379825	40.0	37.0	40.0	32.0	40.0
52	37.42031874646468	40.0	37.0	40.0	33.0	40.0
53	37.44576131786129	40.0	37.0	40.0	33.0	40.0
54	37.36901255305775	40.0	37.0	40.0	33.0	40.0
55	36.99671076381549	40.0	37.0	40.0	31.0	40.0
56	37.03330351450482	40.0	36.0	40.0	31.0	40.0
57	37.06737170557913	40.0	36.0	40.0	32.0	40.0
58	36.75833379959802	39.0	36.0	40.0	31.0	40.0
59	36.60268577552142	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	1.0
8	10.0
9	38.0
10	105.0
11	303.0
12	639.0
13	1094.0
14	1973.0
15	3669.0
16	7597.0
17	14044.0
18	23303.0
19	35502.0
20	52095.0
21	76788.0
22	114478.0
23	170105.0
24	251664.0
25	367389.0
26	526742.0
27	737733.0
28	1015410.0
29	1367565.0
30	1797021.0
31	2323720.0
32	2965460.0
33	3768826.0
34	4880475.0
35	6535646.0
36	9457318.0
37	1.6313408E7
38	3.9478848E7
39	4.1880173E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.45856305424862	29.43475013476087	26.65690724431325	27.44977956667726
2	22.77442958698782	28.989301213618095	26.646543460443805	21.589725738950275
3	22.476110621053905	26.66294067332606	27.777357868343845	23.083590837276198
4	23.02427690098609	26.357670034457925	26.32136436915305	24.29668869540294
5	24.673665836860863	26.28654935956474	29.373584058742924	19.666200744831468
6	23.12699947707052	26.127611175097094	28.640232873813616	22.10515647401877
7	23.508534298381857	27.284105109026445	27.450439181831847	21.75692141075985
8	22.749271790459304	27.008100513841697	27.42290677074683	22.81972092495217
9	22.623445541423784	26.319617320653226	28.493695454252098	22.56324168367089
10	22.381050015352635	27.62309065356406	26.520493613050807	23.4753657180325
11	22.61508967080456	27.763804081240945	26.7303689940093	22.890737253945193
12	23.100479966544913	27.14867531053694	26.898281671218545	22.8525630516996
13	22.61752614757329	27.412611557040357	27.144272658878055	22.825589636508298
14	23.31898251746305	27.399034664773854	26.64814144337197	22.633841374391128
15	23.174173513204895	27.013222406883823	26.620509158353943	23.192094921557334
16	22.77225099365806	27.262779043017364	27.09778954166831	22.867180421656265
17	23.243931728773134	27.0997408100013	26.593291275625123	23.063036185600442
18	22.72069293906126	27.0752597711681	27.194586761279382	23.009460528491264
19	23.122927750980715	27.073154219968448	26.539126809507906	23.264791219542932
20	22.952575615691305	27.14596902508351	26.705469080919748	23.195986278305437
21	22.867453956980256	27.2642935492254	26.734998970664964	23.133253523129383
22	22.71898017564292	26.837254226182246	26.944256474828943	23.499509123345895
23	23.32233053020246	27.09759873773659	26.9074678370719	22.67260289498905
24	22.81448648740344	26.80396266673627	26.811740162937465	23.569810682922824
25	22.979803187682283	27.39860237461605	26.720851902586872	22.900742535114798
26	23.762314707488294	26.611267092911223	27.091132273238117	22.535285926362366
27	23.468113677971395	27.022122366839596	26.805419782699214	22.704344172489797
28	24.010180194711385	26.511098010069272	26.864010005638928	22.61471178958041
29	23.31630604512395	26.676917061324602	27.352461362893255	22.65431553065819
30	23.171538779225862	26.878600543792697	26.80113936480909	23.148721312172352
31	23.132796637152257	26.780226955761353	26.69171330996726	23.395263097119134
32	23.150562271982313	27.06392258911574	27.005804158710323	22.779710980191624
33	23.59538288174055	26.55433671511191	27.027256930455312	22.823023472692228
34	23.29051024794874	26.73562504606592	27.08014017798414	22.893724528001197
35	23.728855449274207	26.602465516232744	27.016937120929512	22.65174191356354
36	23.35686306053248	26.623627610113004	27.050455260044405	22.96905406931011
37	23.270223914301965	26.93475727127511	26.831862524455417	22.963156289967507
38	23.31132129240775	26.92222160202663	26.973474817529393	22.792982288036228
39	23.278670714919897	26.87160266053127	26.90421671695406	22.945509907594776
40	23.61906418378181	26.88179650964902	26.711742505502922	22.787396801066247
41	23.144080900926674	26.914861489426073	27.026010742276263	22.915046867370986
42	23.149150621018723	26.79138451379987	27.042991546871548	23.01647331830986
43	23.158778766292038	26.887452802765537	26.8947592517603	23.05900917918213
44	23.19289614900499	26.86125452854685	26.977469029521938	22.96838029292622
45	23.398142298635687	26.71924199441298	26.90056461044847	22.982051096502868
46	23.096978416266698	26.856468778368807	26.910597468748833	23.135955336615663
47	23.209895586796733	26.823390382690306	27.016275269791358	22.950438760721607
48	23.337179697123055	26.713495516625606	26.869301833432747	23.080022952818595
49	23.331686630807503	26.8553969969086	26.742519328755048	23.070397043528853
50	23.26273262399835	26.834034409834455	26.983850526644567	22.919382439522625
51	23.25997938288985	26.691561263084168	26.984680076550838	23.06377927747515
52	23.19435102898436	26.823824908831682	26.900115923077784	23.081708139106173
53	23.180996244419628	26.80672037981192	26.92777727588228	23.08450609988617
54	23.147337238339517	26.769929506071303	26.898061054172494	23.184672201416685
55	23.27508643324941	26.80157761758976	26.926131591971185	22.99720435718964
56	23.192971427118678	26.859745984961688	26.88348542257589	23.06379716534375
57	23.17950707935878	26.830455345458976	26.84163377267752	23.148403802504724
58	23.580000060073424	26.83285231985122	26.62044058819098	22.966707031884372
59	23.332836671692835	26.868041484024385	26.76152295315772	23.037598891125064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2799.0
1	2071.0
2	1343.0
3	1434.0
4	1743.5
5	1962.0
6	2302.0
7	2642.0
8	3092.0
9	4726.5
10	5911.0
11	8856.5
12	11802.0
13	16924.5
14	32446.0
15	42845.0
16	66364.5
17	89884.0
18	143486.0
19	309645.0
20	422202.0
21	492801.0
22	563400.0
23	720658.5
24	877917.0
25	1025915.5
26	1389969.0
27	1606024.0
28	1860101.0
29	2114178.0
30	2399944.5
31	2974359.5
32	3263008.0
33	3649484.5
34	4035961.0
35	4422711.5
36	5188376.0
37	5567290.0
38	6065658.0
39	6564026.0
40	7067446.0
41	7570866.0
42	7928229.0
43	8564782.0
44	8843972.0
45	8963204.0
46	9082436.0
47	9169408.5
48	9202120.5
49	9147860.0
50	9008969.5
51	8870079.0
52	8533102.5
53	7766845.5
54	7337565.0
55	6835938.0
56	6334311.0
57	5675843.5
58	4388306.0
59	3759236.0
60	3297670.5
61	2836105.0
62	2455936.0
63	2075767.0
64	1733343.5
65	1153312.0
66	915704.0
67	723705.5
68	531707.0
69	415179.0
70	242816.5
71	186982.0
72	131724.5
73	76467.0
74	58147.0
75	30234.5
76	20642.0
77	16236.5
78	11831.0
79	9341.0
80	6851.0
81	5429.5
82	3319.5
83	2631.0
84	1843.0
85	1055.0
86	832.5
87	517.0
88	424.0
89	347.0
90	270.0
91	236.0
92	168.5
93	135.0
94	87.5
95	40.0
96	29.5
97	13.0
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.34169143E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.491870311756024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.882830470430484	20.390798623282855
2	17.49299479421149	11.71746226023909
3	7.317525138431537	7.352328088180907
4	3.8953232733336063	5.218466475714164
5	2.341266686321299	3.920670011175386
6	1.6002925794697465	3.215807491947976
7	1.1454285098019272	2.6853780171171873
8	0.8270998574062404	2.216089692729736
9	0.6588749325061578	1.986025841204692
>10	3.50062722513554	20.67214822009755
>50	0.19683897282181734	4.539534996880527
>100	0.11800602129850599	8.202139835373877
>500	0.01592418607958206	3.6362550884114304
>1k	0.006876544995693384	3.99607497341833
>5k	7.73171978552882E-5	0.154143330161833
>10k+	1.349055860705347E-5	0.09667705406428319
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.55269254421637E-4	2.2359835748522297E-6	0.0	0.0	4.471967149704459E-6
2	0.01516220462107297	5.217295007988536E-6	0.0	7.453278582840766E-7	5.9626228662726125E-6
3	0.01607299526389611	9.689262157692994E-6	0.0	2.2359835748522297E-6	8.198606441124841E-6
4	0.018717418505088013	1.1179917874261148E-5	0.0	2.9813114331363063E-6	9.689262157692994E-6
5	0.027595763952967933	1.26705735908293E-5	0.0	5.217295007988536E-6	1.3415901449113378E-5
6	0.07399838575401797	1.4161229307397454E-5	0.0	6.707950724556689E-6	1.6397212882249683E-5
7	0.07418248173501414	1.6397212882249683E-5	0.0	7.453278582840766E-6	1.7887868598817838E-5
8	0.07447688623903635	1.8633196457101915E-5	0.0	7.453278582840766E-6	1.937852431538599E-5
9	0.0745588723034476	2.0123852173670066E-5	0.0	1.3415901449113378E-5	2.0869180031954144E-5
10	0.07489874180682514	2.0123852173670066E-5	0.0	1.6397212882249683E-5	2.4595819323374527E-5
11	0.07492557360972336	2.161450789023822E-5	0.0	1.8633196457101915E-5	2.608647503994268E-5
12	0.07493377221616448	2.608647503994268E-5	0.0	1.937852431538599E-5	3.2794425764499365E-5
13	0.07494569746189703	2.8322458614794908E-5	0.0	2.3105163606806373E-5	3.726639291420383E-5
14	0.07497401992051182	3.204909790621529E-5	0.0	2.4595819323374527E-5	4.77009829301809E-5
15	0.07500085172341005	3.2794425764499365E-5	0.0	2.53411471816586E-5	4.993696650503313E-5
16	0.07502544754273342	3.428508148106752E-5	0.0	2.6831802898226756E-5	5.8880900804442044E-5
17	0.07505749664063964	3.428508148106752E-5	0.0	2.8322458614794908E-5	6.186221223757835E-5
18	0.07510966959071953	3.5030409339351594E-5	0.0	3.0558442189647137E-5	6.186221223757835E-5
19	0.07524084729377753	4.024770434734013E-5	0.0	3.130377004793122E-5	6.33528679541465E-5
20	0.0755784808135802	4.099303220562421E-5	0.0	3.204909790621529E-5	7.006081867870319E-5
21	0.07578866326961632	4.173836006390829E-5	0.0	3.204909790621529E-5	7.527811368669173E-5
22	0.075869158678311	4.322901578047644E-5	0.0	3.3539753622783446E-5	8.645803156095288E-5
23	0.0759392194969897	4.6210327213612745E-5	0.0	3.428508148106752E-5	9.018467085237326E-5
24	0.07608679441292995	5.06822943633172E-5	0.0	3.950237648905606E-5	9.018467085237326E-5
25	0.07617996039521546	5.291827793816943E-5	0.0	4.024770434734013E-5	9.167532656894141E-5
26	0.07627759834465067	5.6644917229589815E-5	0.0	4.322901578047644E-5	9.54019658603618E-5
27	0.0763841802283853	5.7390245087873896E-5	0.0	4.471967149704459E-5	9.614729371864587E-5
28	0.0765153579314433	6.03715565210102E-5	0.0	4.471967149704459E-5	1.0061926086835034E-4
29	0.0775029173436697	6.111688437929427E-5	0.0	4.6210327213612745E-5	1.0583655587633886E-4
30	0.07765496422675965	6.186221223757835E-5	0.0	4.77009829301809E-5	1.110538508843274E-4
31	0.07785173078134665	6.484352367071466E-5	0.0	4.8446310788464974E-5	1.1478049017574779E-4
32	0.0782616611034029	6.782483510385096E-5	0.0	4.9191638646749055E-5	1.2819639162486117E-4
33	0.07904052871530975	6.931549082041912E-5	0.0	5.1427622221601284E-5	1.4012163735740638E-4
34	0.07932747994074912	7.30421301118395E-5	0.0	5.291827793816943E-5	1.4012163735740638E-4
35	0.07971952239420654	7.676876940325989E-5	0.0	5.291827793816943E-5	1.5875483381450832E-4
36	0.07995653665314088	7.825942511982804E-5	0.0	5.515426151302167E-5	1.6993475168876945E-4
37	0.08037317492592168	8.049540869468027E-5	0.0	5.515426151302167E-5	1.7366139098018982E-4
38	0.08064596492205366	8.422204798610066E-5	0.0	6.33528679541465E-5	1.7813335812989428E-4
39	0.08090161237744509	8.645803156095288E-5	0.0	6.484352367071466E-5	1.7887868598817836E-4
40	0.0812243393400821	8.720335941923695E-5	0.0	7.155147439527135E-5	1.811146695630306E-4
41	0.0816029658920904	9.092999871065734E-5	0.0	7.378745797012358E-5	1.8558663671273506E-4
42	0.08204867195134428	9.465663800207772E-5	0.0	7.602344154497581E-5	1.8931327600415544E-4
43	0.08257487341929284	9.614729371864587E-5	0.0	7.751409726154396E-5	1.937852431538599E-4
44	0.08310927349368252	9.912860515178218E-5	0.0	7.975008083639618E-5	2.0571048888640513E-4
45	0.08359671791300032	1.0285524444320257E-4	0.0	8.198606441124841E-5	2.1763573461895035E-4
46	0.08414676987241396	1.0583655587633886E-4	7.453278582840766E-7	8.347672012781658E-5	2.2285302962693888E-4
47	0.08476390133907317	1.0658188373462294E-4	7.453278582840766E-7	9.018467085237326E-5	2.288156524932115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	34650	0.0	45.267967	1
TACGTTA	33965	0.0	42.552925	1
CGGTAAG	23435	0.0	40.109024	3
CGTTAAG	27650	0.0	39.98481	3
ACGGTAA	26090	0.0	37.662704	2
ACGTTAA	32955	0.0	37.327568	2
TCGCTTC	38830	0.0	34.99665	1
GACGGTA	31265	0.0	33.437553	1
CGCTTAG	16620	0.0	33.117027	2
TACGTTT	27425	0.0	32.167183	1
CGCTTAA	15240	0.0	31.45571	2
GGTAAGC	56920	0.0	29.852074	4
TCGCTTG	31500	0.0	29.17524	1
GTTAAGC	42680	0.0	28.909092	4
TCGCTTT	41705	0.0	28.275988	1
CGCTTAT	13880	0.0	27.60735	2
TAAGCAG	120475	0.0	26.749657	6
CGCTTAC	11740	0.0	26.048552	2
TCGCGTC	6235	0.0	24.693665	1
CGCGTAG	3960	0.0	24.157827	2
>>END_MODULE