FastQCFastQC Report
Thu 26 May 2016
SRR805338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124946227
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA445850.041.558371
TAGCCTA1030950.037.5313071
GCGATTA374100.036.163061
GCGATTC328500.035.917811
CGCTTAG198700.034.9224972
TCGCTTC442050.034.6261751
TATACTC707050.034.0941931
TATACTA1024050.033.768371
TATACTT909300.033.6387331
CGCTTAA183200.033.407752
CCTAAGC570200.031.4505444
CTAAGCA964050.031.184385
CGATTCG54650.031.059472
ACTAAGC434150.030.74264
GCCTAAG546950.030.5419143
TAGCCTC861450.030.199431
TCGCTTG343600.030.1423191
AGCCTAA828400.029.9438672
GCTCTTA1117150.029.5892221
TAGCCTT1129650.029.4674471
Produced by FastQC (version 0.11.5)