##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124946227
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.556847730984305	31.0	28.0	33.0	16.0	34.0
2	30.224541290070327	31.0	30.0	34.0	25.0	34.0
3	27.257822279019276	31.0	25.0	34.0	16.0	34.0
4	29.94049088813222	35.0	26.0	37.0	16.0	37.0
5	32.27219678270077	35.0	32.0	37.0	19.0	37.0
6	33.94897720280901	35.0	33.0	37.0	28.0	37.0
7	34.81474238513821	35.0	35.0	37.0	32.0	37.0
8	35.167457509541286	37.0	35.0	37.0	33.0	37.0
9	37.20167198005907	39.0	37.0	39.0	34.0	39.0
10	37.369782226397284	39.0	37.0	39.0	34.0	39.0
11	37.406853269767	39.0	37.0	39.0	34.0	39.0
12	37.43611015160946	39.0	37.0	39.0	35.0	39.0
13	37.44411718810845	39.0	37.0	39.0	35.0	39.0
14	38.40661861682306	40.0	38.0	40.0	35.0	40.0
15	38.438810025051815	40.0	38.0	40.0	35.0	40.0
16	38.30174891955721	40.0	38.0	40.0	35.0	40.0
17	38.34472179780187	40.0	38.0	40.0	35.0	40.0
18	38.37777929060635	40.0	38.0	40.0	35.0	40.0
19	38.47951117403489	40.0	39.0	40.0	35.0	40.0
20	38.28236382840116	40.0	38.0	40.0	34.0	40.0
21	38.3365929809149	40.0	38.0	40.0	34.0	40.0
22	38.37598222953943	40.0	38.0	40.0	34.0	40.0
23	38.35564560905068	40.0	38.0	40.0	34.0	40.0
24	38.33214058556566	40.0	38.0	40.0	34.0	40.0
25	38.31366054774907	40.0	38.0	40.0	34.0	40.0
26	38.18567572272511	40.0	38.0	40.0	34.0	40.0
27	38.163743367776924	40.0	38.0	40.0	34.0	40.0
28	38.0720817924338	40.0	38.0	40.0	34.0	40.0
29	38.05646502635089	40.0	38.0	40.0	34.0	40.0
30	37.952793516526114	40.0	38.0	40.0	34.0	40.0
31	37.945049705262406	40.0	38.0	40.0	34.0	40.0
32	37.89788471163679	40.0	38.0	40.0	34.0	40.0
33	37.52752686961888	40.0	38.0	40.0	33.0	40.0
34	37.373119438012324	40.0	37.0	40.0	32.0	40.0
35	37.23550890416243	40.0	37.0	40.0	31.0	40.0
36	37.224456773712745	40.0	37.0	40.0	31.0	40.0
37	37.03548220787811	40.0	37.0	40.0	31.0	40.0
38	37.05409550301987	40.0	37.0	40.0	31.0	40.0
39	37.15822047991893	40.0	37.0	40.0	31.0	40.0
40	37.1556158074305	40.0	37.0	40.0	31.0	40.0
41	37.112471455420575	40.0	37.0	40.0	31.0	40.0
42	37.24237003170972	40.0	37.0	40.0	32.0	40.0
43	37.22198603884213	40.0	37.0	40.0	31.0	40.0
44	37.14502467529492	40.0	37.0	40.0	31.0	40.0
45	37.053240207085246	40.0	37.0	40.0	31.0	40.0
46	36.654478218057754	40.0	36.0	40.0	30.0	40.0
47	36.50914117638782	40.0	36.0	40.0	30.0	40.0
48	36.633172868837406	40.0	36.0	40.0	30.0	40.0
49	34.4873423748922	39.0	33.0	40.0	19.0	40.0
50	35.475584893011614	39.0	34.0	40.0	25.0	40.0
51	30.777256755420073	37.0	24.0	40.0	9.0	40.0
52	32.665068893997095	37.0	29.0	40.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	16.0
8	238.0
9	613.0
10	1095.0
11	2194.0
12	3653.0
13	5612.0
14	7723.0
15	10872.0
16	15669.0
17	22559.0
18	32645.0
19	47089.0
20	71132.0
21	112126.0
22	180480.0
23	283395.0
24	425397.0
25	615420.0
26	854594.0
27	1145266.0
28	1493448.0
29	1905381.0
30	2396655.0
31	2992557.0
32	3740030.0
33	4748757.0
34	6283766.0
35	9023871.0
36	1.4713751E7
37	2.4775268E7
38	3.4037324E7
39	1.4997631E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.87492950867576	27.65093498981766	31.666766536295647	22.80736896521093
2	19.389847602200906	30.24692854470908	22.851889717326156	27.51133413576386
3	24.298920206690195	21.767428799590725	33.78395811823914	20.149692875479946
4	16.835221442901194	30.88561369684256	21.948058503599313	30.331106356656935
5	19.44577085949142	22.63539018269035	34.013246354369706	23.90559260344852
6	19.602114115858814	22.365185144806333	39.70010074814024	18.332599991194613
7	22.545525924524316	28.071209385138136	27.27160140657949	22.11166328375806
8	23.308913521654397	27.548326849437398	26.98806503376849	22.154694595139716
9	22.675288146155868	26.428710808530457	28.040556198627755	22.855444846685927
10	22.165917022848557	27.773911092169275	26.359054443476715	23.701117441505456
11	22.92642258017123	27.959783051312147	26.544809552352472	22.568984816164157
12	23.46858060788022	27.072173215762653	26.70390119102996	22.755344985327167
13	22.654255898419407	27.50090324856308	27.21527877748561	22.6295620755319
14	23.30613872798256	27.475985329272888	26.66493963038996	22.552936312354593
15	23.205527446619094	26.94054219020155	26.807917937369968	23.046012425809383
16	22.80656862091562	27.299356546396552	27.19924227884048	22.694832553847345
17	23.072996834070068	27.567771214091962	26.706397464887033	22.652834486950933
18	22.506275439593708	27.116249776793982	27.38290848910548	22.994566294506836
19	22.903273421773672	27.193210083886726	26.66417290055505	23.239343593784547
20	23.09049556174273	27.613100313945456	26.65683374336706	22.639570380944757
21	22.8661158371753	27.71875376436937	26.69617066548156	22.71895973297377
22	22.67639822369346	26.8319418720823	26.989622503767162	23.50203740045708
23	23.380793243160518	27.156174151621244	26.815047404352594	22.64798520086565
24	22.673975581511556	26.905854468098507	26.824012060804364	23.596157889585573
25	23.015194368374164	27.57990763498605	26.652435051120033	22.752462945519756
26	23.757842643779874	26.701818695173564	26.9825506615738	22.557787999472765
27	23.292396816432078	27.222091308127293	26.736484007636342	22.749027867804283
28	23.997144787733365	26.663297323895986	26.700711018668855	22.638846869701794
29	23.129601984700184	26.757209723507696	27.49867589038923	22.614512401402887
30	23.036164989599886	27.22936563742737	26.723347956717415	23.01112141625533
31	23.03092513549849	26.809945209469994	26.756658286288225	23.402471368743292
32	23.07307446746671	27.154737533611158	27.10174913885155	22.67043886007058
33	23.103337886305283	26.613333430228348	27.260817567544475	23.022511115921894
34	23.45887163123381	26.809773935790794	26.93402738763772	22.79732704533767
35	23.628037203556374	26.73939726087127	26.922741732729556	22.709823802842802
36	23.505478080582616	26.666218580573865	26.844346408315317	22.9839569305282
37	23.749940844552274	26.86315770063229	26.694335476012416	22.692565978803025
38	23.29906288406772	26.955520633688284	27.01861577620907	22.726800706034926
39	23.305708943095976	26.918161362327492	26.8868262824775	22.88930341209903
40	23.150370118819193	27.11636742740539	26.838776012019956	22.89448644175546
41	23.112164083193964	27.03007110410785	26.999846101795455	22.85791871090273
42	23.095558539754865	26.940722267667994	26.967540204315256	22.996178988261885
43	23.120302784332974	27.034942799833406	26.845705392928753	22.99904902290487
44	23.170574810554303	26.9999917644572	26.952119170433214	22.87731425455528
45	23.15104801043732	26.85648523024229	26.954221674896996	23.038245084423398
46	23.294841868254252	26.888314122522484	26.803010226151123	23.013833783072137
47	23.459033300781464	26.91318642218784	26.82978654489503	22.797993732135666
48	23.16706370013078	26.88093014605395	26.901335724207183	23.050670429608093
49	24.773073779970964	27.20303831183314	26.060374756254145	21.963513151941754
50	23.28174983627157	26.890346196688274	26.93509984899344	22.892804118046715
51	23.77990253359151	30.89409974740574	24.664405432586612	20.661592286416138
52	23.260629550662625	26.891521902458088	26.83599481559375	23.01185373128554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3215.0
1	2933.5
2	2652.0
3	2701.0
4	2750.0
5	3514.0
6	4278.0
7	4657.0
8	5036.0
9	6600.5
10	8165.0
11	12491.5
12	16818.0
13	27220.0
14	59516.0
15	81410.0
16	125022.5
17	168635.0
18	247347.5
19	326060.0
20	457133.0
21	588206.0
22	791105.0
23	994004.0
24	1197913.0
25	1401822.0
26	1663422.0
27	1925022.0
28	2259079.5
29	2593137.0
30	2901647.0
31	3210157.0
32	3578203.0
33	3946249.0
34	4369767.0
35	4793285.0
36	5296344.5
37	5799404.0
38	6285264.5
39	7201254.5
40	7631384.0
41	8058694.0
42	8486004.0
43	8780933.5
44	9075863.0
45	9227605.0
46	9379347.0
47	9406627.5
48	9433908.0
49	9318489.0
50	9203070.0
51	8911112.0
52	8619154.0
53	8140605.0
54	7662056.0
55	7047325.5
56	6432595.0
57	5775406.5
58	5118218.0
59	4481120.0
60	3844022.0
61	3294046.0
62	2744070.0
63	2323031.0
64	1564345.0
65	1226698.0
66	971558.0
67	716418.0
68	560134.5
69	403851.0
70	301478.0
71	199105.0
72	145904.5
73	92704.0
74	69292.5
75	45881.0
76	34779.0
77	23677.0
78	18113.5
79	12550.0
80	9885.0
81	7220.0
82	5506.0
83	3792.0
84	2786.5
85	1781.0
86	1298.0
87	815.0
88	612.5
89	303.5
90	197.0
91	175.5
92	154.0
93	128.5
94	103.0
95	90.5
96	78.0
97	56.5
98	35.0
99	29.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.24946227E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.479111702138866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39043043458236	31.30903818061424
2	14.599953360563278	12.987859127410234
3	5.456430659504489	7.280915663972263
4	2.7826448091508125	4.950782771743835
5	1.6685217114357802	3.7107181790197985
6	1.1019269325016254	2.9407638671001024
7	0.7604020541499075	2.367540553354871
8	0.5427745599005714	1.9313704223117425
9	0.39109390220485407	1.565595842597557
>10	2.098411706794937	16.345579453786033
>50	0.10977636892068308	3.3342194203704136
>100	0.0892960941666722	8.282382547664243
>500	0.006999669780724098	2.031454553809955
>1k	0.0013173990158761582	0.8400954074391136
>5k	1.1110411738116168E-5	0.03509481213877517
>10k+	9.22691539818465E-6	0.0865891966668698
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0022217557637814866	2.401032885931001E-6	0.0	1.6006885906206675E-6	1.6006885906206675E-6
2	0.00781696273229603	3.201377181241335E-6	0.0	2.401032885931001E-6	1.5206541610896341E-5
3	0.008698141801432708	3.201377181241335E-6	0.0	6.40275436248267E-6	1.8407918792137677E-5
4	0.00920556008465946	3.201377181241335E-6	0.0	1.2005164429655007E-5	2.0008607382758345E-5
5	0.02179497584989101	4.001721476551669E-6	0.0	1.280550872496534E-5	2.2409640268689346E-5
6	0.14865995113241795	5.602410067172337E-6	0.0	2.0008607382758345E-5	2.561101744993068E-5
7	0.14879120759684883	6.40275436248267E-6	0.0	2.0808951678068678E-5	2.6411361745241016E-5
8	0.14880481344986912	6.40275436248267E-6	0.0	2.320998456399968E-5	2.801205033586168E-5
9	0.14882322136866125	8.003442953103338E-6	0.0	2.4010328859310015E-5	3.0413083221792682E-5
10	0.14888244684651422	1.2005164429655007E-5	0.0	2.6411361745241016E-5	3.521514899365469E-5
11	0.148902455453897	1.5206541610896341E-5	8.003442953103338E-7	2.721170604055135E-5	3.841652617489602E-5
12	0.1489288668156422	1.7607574496827344E-5	8.003442953103338E-7	3.0413083221792682E-5	4.481928053737869E-5
13	0.14894647439013906	1.7607574496827344E-5	8.003442953103338E-7	3.2814116107723684E-5	5.122203489986136E-5
14	0.14897048471899835	2.160929597337901E-5	8.003442953103338E-7	3.3614460403034016E-5	5.7624789262344035E-5
15	0.14899129367067643	2.2409640268689346E-5	1.6006885906206675E-6	3.601549328896502E-5	6.002582214827503E-5
16	0.1490273091639654	2.320998456399968E-5	1.6006885906206675E-6	4.1617903356137356E-5	6.322719932951636E-5
17	0.14909213705188554	2.320998456399968E-5	1.6006885906206675E-6	4.481928053737869E-5	6.802926510137837E-5
18	0.149191379744504	2.320998456399968E-5	2.401032885931001E-6	4.641996912799936E-5	7.283133087324037E-5
19	0.14993970166061918	2.561101744993068E-5	2.401032885931001E-6	5.042169060455103E-5	7.843374094041272E-5
20	0.15009816983109062	2.6411361745241016E-5	2.401032885931001E-6	5.122203489986136E-5	8.483649530289538E-5
21	0.15076165525190288	2.801205033586168E-5	2.401032885931001E-6	5.282272349048203E-5	8.803787248413672E-5
22	0.15092172411096494	3.0413083221792682E-5	2.401032885931001E-6	5.44234120811027E-5	9.604131543724006E-5
23	0.15098975337606632	3.3614460403034016E-5	2.401032885931001E-6	5.7624789262344035E-5	9.844234832317105E-5
24	0.15158280849889127	3.521514899365469E-5	2.401032885931001E-6	6.40275436248267E-5	1.0244406979972272E-4
25	0.15172046771768466	3.7616181879585686E-5	2.401032885931001E-6	6.64285765107577E-5	1.0644579127627439E-4
26	0.15189094105258577	3.921687047020636E-5	2.401032885931001E-6	7.203098657793004E-5	1.0964716845751573E-4
27	0.15208222333916493	4.081755906082702E-5	2.401032885931001E-6	7.683305234979204E-5	1.1444923422937773E-4
28	0.152219082213663	4.1617903356137356E-5	3.201377181241335E-6	8.163511812165405E-5	1.2005164429655006E-4
29	0.1523727483183626	4.1617903356137356E-5	3.201377181241335E-6	8.323580671227471E-5	1.2645439865903273E-4
30	0.1525512250962168	4.481928053737869E-5	3.201377181241335E-6	8.643718389351604E-5	1.2725474295434308E-4
31	0.15276571736735997	4.722031342330969E-5	3.201377181241335E-6	9.043890537006771E-5	1.2725474295434308E-4
32	0.15318349708951196	4.9621346309240695E-5	3.201377181241335E-6	9.364028255130906E-5	1.3525818590744642E-4
33	0.15463772267409084	5.282272349048203E-5	3.201377181241335E-6	9.764200402786073E-5	1.400602516793084E-4
34	0.15498587244255083	5.282272349048203E-5	3.201377181241335E-6	9.924269261848139E-5	1.4406197315586008E-4
35	0.15546928039691826	5.6824444967033695E-5	4.001721476551669E-6	1.0244406979972272E-4	1.5046472751834274E-4
36	0.1557365953915519	5.92254778529647E-5	4.001721476551669E-6	1.0404475839034339E-4	1.6006885906206676E-4
37	0.15606313586403853	6.16265107388957E-5	4.001721476551669E-6	1.0724613557158473E-4	1.6407058053861842E-4
38	0.1564481014700828	6.322719932951636E-5	4.001721476551669E-6	1.2885543154496373E-4	1.6887264631048043E-4
39	0.15684747327344267	6.64285765107577E-5	5.602410067172337E-6	1.3365749731682574E-4	1.7367471208234244E-4
40	0.15722043371505728	6.722892080606803E-5	6.40275436248267E-6	1.968846966463421E-4	1.7447505637765276E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	44585	0.0	41.55837	1
TAGCCTA	103095	0.0	37.531307	1
GCGATTA	37410	0.0	36.16306	1
GCGATTC	32850	0.0	35.91781	1
CGCTTAG	19870	0.0	34.922497	2
TCGCTTC	44205	0.0	34.626175	1
TATACTC	70705	0.0	34.094193	1
TATACTA	102405	0.0	33.76837	1
TATACTT	90930	0.0	33.638733	1
CGCTTAA	18320	0.0	33.40775	2
CCTAAGC	57020	0.0	31.450544	4
CTAAGCA	96405	0.0	31.18438	5
CGATTCG	5465	0.0	31.05947	2
ACTAAGC	43415	0.0	30.7426	4
GCCTAAG	54695	0.0	30.541914	3
TAGCCTC	86145	0.0	30.19943	1
TCGCTTG	34360	0.0	30.142319	1
AGCCTAA	82840	0.0	29.943867	2
GCTCTTA	111715	0.0	29.589222	1
TAGCCTT	112965	0.0	29.467447	1
>>END_MODULE