FastQCFastQC Report
Thu 26 May 2016
SRR805337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102560296
Sequences flagged as poor quality0
Sequence length59
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT17348251.6915171539676521Illumina Paired End PCR Primer 2 (100% over 56bp)
TGAACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT1987670.19380501787943358Illumina Paired End PCR Primer 2 (100% over 54bp)
GCGCTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT1659270.1617848294821614Illumina Paired End PCR Primer 2 (100% over 54bp)
CGGAGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT1048820.1022637454166474Illumina Paired End PCR Primer 2 (100% over 54bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT2248650.046.3545231
TAGATCG362100.046.2085085
GCGCTAG219700.045.4371871
GAGATCG2346200.045.019053
CGTCTTC2560000.041.99421752
GGAGATC2598350.040.4422452
TCGCTTA143400.040.1195951
CGCTAGA256850.039.6287732
GTCTTCT2753450.039.01590353
ACCGATC2909450.037.13069537
GACCGAT2934550.036.8889636
AATGCCG2983700.036.75734728
CGAGACC2978350.036.6240733
GAGACCG2980050.036.59072534
AGATCGG3054500.036.5551644
CTCGTAT2940500.036.46735443
GATCGGA3075550.036.463515
TCTCGTA2965250.036.44537442
CGGTTCA3039050.036.42447716
AGACCGA2984450.036.40181735
Produced by FastQC (version 0.11.5)