FastQCFastQC Report
Thu 26 May 2016
SRR805336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17893911
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3804232.125991349794911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG521750.2915796328706452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG324670.1814416088243649No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG192110.10736054292435009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC184340.10301828370555771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1787050.044.96435512
AAGCAGT1798800.044.9162521
GTGGTAT1790900.044.899186
CAACGCA1792100.044.85207413
AGTGGTA1790550.044.8082165
GTATCAA1784650.044.77999
GGTATCA1787300.044.7056168
ACGCAGA1809850.044.6212415
TGGTATC1796500.044.600957
ATCAACG1800850.044.58716611
AACGCAG1806050.044.581114
TATCAAC1801650.044.43823610
CGCAGAG1818600.044.34193816
AGCAGTG1835650.044.0849842
AGAGTAC1790250.044.05569519
CAGTGGT1831450.043.890964
GCAGTGG1842550.043.8255123
GCAGAGT1825250.043.57011417
CAGAGTA1817150.043.55223518
ACATGGG1178450.043.0316524
Produced by FastQC (version 0.11.5)