##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805336_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17893911
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.58444691046021	37.0	35.0	37.0	35.0	37.0
2	35.73848998131264	37.0	35.0	37.0	35.0	37.0
3	37.43734832480166	39.0	37.0	39.0	35.0	39.0
4	37.46637166128746	39.0	37.0	39.0	35.0	39.0
5	37.40917605994576	39.0	37.0	39.0	35.0	39.0
6	37.34988633843099	39.0	37.0	39.0	34.0	39.0
7	37.1716891852206	39.0	37.0	39.0	34.0	39.0
8	38.08945473127702	40.0	38.0	40.0	34.0	40.0
9	38.01464207573179	40.0	38.0	40.0	34.0	40.0
10	37.78444024897632	40.0	37.0	40.0	33.0	40.0
11	37.91748193002636	40.0	38.0	40.0	33.0	40.0
12	37.9406215890981	40.0	38.0	40.0	33.0	40.0
13	37.97638889564165	40.0	38.0	40.0	34.0	40.0
14	37.86436699053662	40.0	38.0	40.0	33.0	40.0
15	37.82828773430247	40.0	38.0	40.0	33.0	40.0
16	37.774623613585646	40.0	37.0	40.0	33.0	40.0
17	37.679169746624986	40.0	37.0	40.0	33.0	40.0
18	37.66058621840692	40.0	37.0	40.0	33.0	40.0
19	37.633015219534734	40.0	37.0	40.0	33.0	40.0
20	37.431102457143105	40.0	37.0	40.0	32.0	40.0
21	37.34647266324282	40.0	37.0	40.0	32.0	40.0
22	37.29773809649551	40.0	37.0	40.0	32.0	40.0
23	36.89439049965097	39.0	36.0	40.0	31.0	40.0
24	37.06149047013814	39.0	36.0	40.0	31.0	40.0
25	37.07069773622994	39.0	36.0	40.0	31.0	40.0
26	37.02073319801356	39.0	36.0	40.0	31.0	40.0
27	36.95852231521661	39.0	36.0	40.0	31.0	40.0
28	36.85803729548001	39.0	36.0	40.0	31.0	40.0
29	36.763674246507655	39.0	36.0	40.0	30.0	40.0
30	36.66402442708025	39.0	36.0	40.0	30.0	40.0
31	36.47416783284549	39.0	36.0	40.0	30.0	40.0
32	36.33169093106588	39.0	35.0	40.0	30.0	40.0
33	36.19330748878767	38.0	35.0	40.0	30.0	40.0
34	36.084361657996396	38.0	35.0	40.0	30.0	40.0
35	35.94296506783788	38.0	35.0	40.0	29.0	40.0
36	36.06306592225702	38.0	35.0	40.0	30.0	40.0
37	36.02285179578685	38.0	35.0	40.0	30.0	40.0
38	36.010193411602415	38.0	35.0	40.0	30.0	40.0
39	35.96846089152897	38.0	35.0	40.0	30.0	40.0
40	35.94481832395389	38.0	35.0	40.0	29.0	40.0
41	35.901548521170135	38.0	35.0	40.0	29.0	40.0
42	35.8108855576626	38.0	35.0	40.0	29.0	40.0
43	35.72020907000152	38.0	34.0	40.0	28.0	40.0
44	35.81828774044981	38.0	35.0	40.0	29.0	40.0
45	35.78013191191126	38.0	35.0	40.0	28.0	40.0
46	35.76527736166789	38.0	35.0	40.0	28.0	40.0
47	35.76487320183944	38.0	35.0	40.0	28.0	40.0
48	35.666664487154314	38.0	34.0	40.0	28.0	40.0
49	35.52758717756001	38.0	34.0	40.0	28.0	40.0
50	35.340874110751976	38.0	34.0	40.0	27.0	40.0
51	35.17051565753289	38.0	34.0	40.0	27.0	40.0
52	34.899988884487016	38.0	34.0	40.0	26.0	40.0
53	34.49526573592548	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	13.0
13	40.0
14	113.0
15	325.0
16	759.0
17	1890.0
18	4089.0
19	7909.0
20	14429.0
21	23867.0
22	36891.0
23	53239.0
24	73994.0
25	100038.0
26	131425.0
27	168453.0
28	213041.0
29	266064.0
30	333146.0
31	413653.0
32	521628.0
33	663950.0
34	873037.0
35	1190383.0
36	1699296.0
37	2556883.0
38	3819952.0
39	4725398.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.628663962841886	34.09026120673116	30.744760047146762	21.53631478328019
2	24.950817068443	36.96336703585929	19.930243310140526	18.155572585557174
3	32.22056933221586	24.263309457613822	22.16859131578334	21.34752989438698
4	21.058772450583888	24.00685350452453	22.729810157209343	32.20456388768224
5	21.76054748456053	33.76808457357366	23.81001559692568	20.66135234494013
6	31.420000915395185	24.100505473621723	22.809513247271656	21.66998036371143
7	22.044487647222567	23.731016657006958	33.407934129101235	20.816561566669243
8	30.764470662674025	24.382092880645263	23.755499845729645	21.097936610951066
9	30.549643395454464	24.02841949979521	24.262661192402266	21.15927591234806
10	21.28348017378649	24.26162173266649	33.3621476042884	21.09275048925861
11	20.95655332140637	33.766123012459374	24.040172101001282	21.237151565132965
12	20.80366891284974	24.22177577612854	33.72374546850043	21.25080984252129
13	21.00106008127569	24.15484239303526	24.13198545583467	30.712112069854374
14	21.487840193236682	33.696339497832525	23.8089649602035	21.006855348727285
15	20.987178264159244	33.698127815657514	23.964811270157764	21.349882650025474
16	21.130567822763844	24.131543964871625	23.950415311666635	30.7874729006979
17	30.875519611112406	23.859607885609805	23.984398938834556	21.280473564443234
18	21.403062751345974	23.92281933222983	23.80250466206074	30.871613254363456
19	21.266044074992884	33.4859215517502	23.828021722026	21.420012651230913
20	31.02848225857388	23.929547878046336	23.826211050228203	21.21575881315158
21	21.58392315687722	33.34805901292345	23.804712116875958	21.263305713323373
22	30.81441502642994	23.793076874027147	24.178386714899833	21.21412138464308
23	21.503236492011165	23.849861553463633	33.34156518382147	21.305336770703732
24	21.36288707370904	33.23270133622549	23.977955406171407	21.426456183894064
25	21.196154378995182	24.010100419075517	24.009725990030912	30.784019211898396
26	21.477529423277	29.94461635580953	27.193619103168672	21.3842351177448
27	21.542205055116234	23.84601108164671	33.34069337888179	21.271090484355266
28	27.683752311051506	23.775758133590806	27.23580104986551	21.304688505492177
29	27.756581554474035	23.834979396063837	26.99186332155111	21.41657572791102
30	27.60393186263193	23.82827320422014	27.145384818332897	21.422410114815037
31	25.693583700064227	24.624935264291857	27.657598162861095	22.023882872782814
32	24.62213542919712	25.471994356068944	27.702764364928385	22.203105849805556
33	24.11581235650496	25.17059574064049	27.987805460751424	22.72578644210313
34	23.423677473303627	25.039757937770002	28.33493471606068	23.20162987286569
35	23.188295728083144	25.122920305125024	28.690167286514388	22.998616680277443
36	23.34672392189723	25.261844657660365	28.659123206771287	22.73230821367112
37	23.85162751731581	25.09191534483434	28.456439735282018	22.600017402567836
38	23.853477308566028	25.26135287025849	28.447157248071704	22.438012573103777
39	23.75892000356993	25.24770018136337	28.45342753744556	22.539952277621143
40	23.34172780897368	25.71571413314842	28.22609322243751	22.71646483544039
41	23.720046444849313	25.805437391523856	28.013177219893404	22.46133894373343
42	23.54276826346124	25.799524765715	28.2304354816563	22.42727148916746
43	23.281685037999797	25.5034966922547	28.79398472474799	22.420833544997514
44	23.74625088947855	25.192709408245072	28.420181591380445	22.640858110895937
45	23.87410443697859	24.87954142613093	28.329072386690648	22.91728175019983
46	23.475242500088438	24.967811676273566	28.178205424180327	23.37874039945767
47	23.36276289739007	25.060591840431083	28.374864499996676	23.201780762182175
48	23.073781913858856	25.102930264937612	28.34589933972512	23.47738848147842
49	22.885164679761736	25.756471014078475	28.083156331782362	23.27520797437743
50	23.657405024536	25.59939523561954	27.869955316084894	22.873244423759566
51	23.540069021244154	25.256418230760175	28.183559200668874	23.019953547326796
52	23.81536937341423	25.149230931125118	27.96287519257249	23.07252450288816
53	24.221680771744087	25.349248691356518	27.459636968128432	22.969433568770963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7266.0
1	4398.0
2	1530.0
3	1372.5
4	1215.0
5	1355.0
6	1495.0
7	1764.5
8	2474.5
9	2915.0
10	4022.5
11	5130.0
12	7108.0
13	9086.0
14	11572.5
15	14059.0
16	20317.0
17	26575.0
18	35462.0
19	44349.0
20	62220.0
21	80091.0
22	310611.0
23	541131.0
24	392373.5
25	253896.0
26	264176.0
27	287349.0
28	310522.0
29	331212.5
30	351903.0
31	371975.0
32	392047.0
33	431148.5
34	470250.0
35	521631.0
36	573012.0
37	624852.5
38	676693.0
39	728345.0
40	779997.0
41	835782.5
42	941055.0
43	990542.0
44	1043220.5
45	1095899.0
46	1140968.0
47	1186037.0
48	1220379.5
49	1254722.0
50	1258703.0
51	1262684.0
52	1255009.5
53	1247335.0
54	1215262.5
55	1183190.0
56	1155796.5
57	1128403.0
58	1008077.0
59	773302.5
60	658854.0
61	571074.5
62	483295.0
63	412129.5
64	340964.0
65	277467.5
66	213971.0
67	171339.5
68	128708.0
69	100613.0
70	72518.0
71	53867.0
72	35216.0
73	26021.5
74	16827.0
75	12792.0
76	6455.5
77	4154.0
78	3015.0
79	1876.0
80	1401.0
81	926.0
82	646.5
83	367.0
84	246.0
85	125.0
86	93.5
87	62.0
88	44.5
89	27.0
90	22.5
91	18.0
92	18.0
93	10.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7893911E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.88398343995827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72242898205886	41.87994076182592
2	12.363469572798172	13.323859794421693
3	3.8705782013149004	6.256865151081467
4	1.7494019684541278	3.770589867920505
5	0.9869089694176042	2.658929328242224
6	0.636083774290241	2.0564836536168905
7	0.44483333328438024	1.6778574374965889
8	0.3226980211878799	1.391060386383602
9	0.248773184848318	1.2064401155405202
>10	1.5185708363314974	14.88544606651504
>50	0.09750553468548573	3.6325648411666718
>100	0.037425933587289996	3.3333909406086915
>500	7.944868669066787E-4	0.2707002898948036
>1k	3.751566910218483E-4	0.47489016284288316
>5k	7.095395219496504E-5	0.23749132840306836
>10k+	8.109023107995969E-5	2.943489874039398
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	380423	2.125991349794911	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	52175	0.2915796328706452	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	32467	0.1814416088243649	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	19211	0.10736054292435009	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	18434	0.10301828370555771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6265918054471155E-4	2.2353972812315875E-5	0.0	0.0	4.470794562463175E-5
2	4.359024698401596E-4	2.7942466015394847E-5	0.0	0.0	5.029643882771072E-5
3	4.359024698401596E-4	2.7942466015394847E-5	0.0	0.0	5.5884932030789693E-5
4	4.359024698401596E-4	3.9119452421552786E-5	0.0	0.0	6.147342523386866E-5
5	6.985616503848712E-4	4.470794562463175E-5	5.588493203078969E-6	0.0	7.823890484310557E-5
6	7.823890484310557E-4	5.029643882771072E-5	5.588493203078969E-6	0.0	9.500438445234248E-5
7	7.991545280402926E-4	5.029643882771072E-5	5.588493203078969E-6	0.0	1.1735835726465835E-4
8	8.103315144464506E-4	5.029643882771072E-5	5.588493203078969E-6	0.0	1.3412383687389526E-4
9	8.103315144464506E-4	5.5884932030789693E-5	5.588493203078969E-6	0.0	1.3412383687389526E-4
10	8.382739804618453E-4	5.5884932030789693E-5	5.588493203078969E-6	0.0	1.3971233007697422E-4
11	8.885704192895561E-4	6.706191843694763E-5	5.588493203078969E-6	0.0	1.620663028892901E-4
12	9.779863105388196E-4	7.823890484310557E-5	5.588493203078969E-6	5.588493203078969E-6	1.78831782498527E-4
13	0.0013971233007697423	7.823890484310557E-5	5.588493203078969E-6	5.588493203078969E-6	1.9000876890468496E-4
14	0.0015927205628775063	7.823890484310557E-5	5.588493203078969E-6	5.588493203078969E-6	2.011857553108429E-4
15	0.0019336186482653234	7.823890484310557E-5	5.588493203078969E-6	5.588493203078969E-6	2.179512349200798E-4
16	0.0020286230327176656	7.823890484310557E-5	1.1176986406157938E-5	1.1176986406157938E-5	2.4589370093547466E-4
17	0.0020565654987330607	8.382739804618454E-5	1.1176986406157938E-5	1.1176986406157938E-5	2.514821941385536E-4
18	0.0022409857744346666	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	2.6265918054471155E-4
19	0.0022801052268562193	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	2.6265918054471155E-4
20	0.0023136361860746934	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	2.682476737477905E-4
21	0.0023695211181054827	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	2.906016465601064E-4
22	0.002403052077323957	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	3.0177863296626436E-4
23	0.0024645255025578256	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	3.3530959218473815E-4
24	0.002514821941385536	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	3.408980853878171E-4
25	0.0025762953666194047	9.500438445234248E-5	1.6765479609236907E-5	1.6765479609236907E-5	3.464865785908961E-4
26	0.0026992422170871422	9.500438445234248E-5	2.2353972812315875E-5	2.2353972812315875E-5	3.57663564997054E-4
27	0.0031127907141149857	1.0618137085850041E-4	2.2353972812315875E-5	2.2353972812315875E-5	3.6325205820013297E-4
28	0.00314632167333346	1.0618137085850041E-4	2.2353972812315875E-5	2.2353972812315875E-5	4.135484970278437E-4
29	0.0033363304422381447	1.0618137085850041E-4	2.2353972812315875E-5	2.2353972812315875E-5	4.303139766370806E-4
30	0.003453688799502803	1.0618137085850041E-4	2.2353972812315875E-5	2.2353972812315875E-5	4.526679494493965E-4
31	0.0035822241431736194	1.1176986406157939E-4	2.2353972812315875E-5	2.2353972812315875E-5	4.526679494493965E-4
32	0.0036939940072351985	1.1176986406157939E-4	2.2353972812315875E-5	2.2353972812315875E-5	4.694334290586334E-4
33	0.00379458688489062	1.1176986406157939E-4	2.2353972812315875E-5	2.2353972812315875E-5	5.029643882771072E-4
34	0.0039007682557491204	1.1176986406157939E-4	2.2353972812315875E-5	2.2353972812315875E-5	5.141413746832652E-4
35	0.004029303599419937	1.1735835726465835E-4	2.2353972812315875E-5	2.2353972812315875E-5	5.36495347495581E-4
36	0.004258431820746174	1.1735835726465835E-4	2.2353972812315875E-5	2.2353972812315875E-5	5.700263067140548E-4
37	0.004398144150823149	1.1735835726465835E-4	2.2353972812315875E-5	2.2353972812315875E-5	6.091457591356076E-4
38	0.004565798946915518	1.2294685046773733E-4	2.7942466015394847E-5	2.2353972812315875E-5	6.091457591356076E-4
39	0.004655214838164781	1.2294685046773733E-4	2.7942466015394847E-5	2.2353972812315875E-5	6.147342523386866E-4
40	0.0047502192226171235	1.2294685046773733E-4	2.7942466015394847E-5	2.2353972812315875E-5	6.314997319479235E-4
41	0.004845223607069466	1.2294685046773733E-4	2.7942466015394847E-5	2.2353972812315875E-5	6.314997319479235E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	178705	0.0	44.964355	12
AAGCAGT	179880	0.0	44.916252	1
GTGGTAT	179090	0.0	44.89918	6
CAACGCA	179210	0.0	44.852074	13
AGTGGTA	179055	0.0	44.808216	5
GTATCAA	178465	0.0	44.7799	9
GGTATCA	178730	0.0	44.705616	8
ACGCAGA	180985	0.0	44.62124	15
TGGTATC	179650	0.0	44.60095	7
ATCAACG	180085	0.0	44.587166	11
AACGCAG	180605	0.0	44.5811	14
TATCAAC	180165	0.0	44.438236	10
CGCAGAG	181860	0.0	44.341938	16
AGCAGTG	183565	0.0	44.084984	2
AGAGTAC	179025	0.0	44.055695	19
CAGTGGT	183145	0.0	43.89096	4
GCAGTGG	184255	0.0	43.825512	3
GCAGAGT	182525	0.0	43.570114	17
CAGAGTA	181715	0.0	43.552235	18
ACATGGG	117845	0.0	43.03165	24
>>END_MODULE