FastQCFastQC Report
Thu 26 May 2016
SRR805335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18302746
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3815412.0846106917508442No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1593150.8704431564531354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG555050.30326050528155724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG401150.21917476208214876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC295660.16153860191252176No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG249660.13640576119015146No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCACGCAGAGTAAGCAGTG242340.13240636131867864No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG231230.12633623391812354No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA212190.11593342332347288No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA204740.11186299585865421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC2132400.044.09501319
GTACATG1420800.043.55968522
GAGTACA1448300.043.31347320
ACATGGG1402900.043.3080824
GAGTACT720450.043.08904320
TACATGG1427250.043.0598723
AGTACAT1445850.043.01141421
GTACTTT714450.042.79963722
CATGGGG873500.042.67395825
AGTACTT731400.042.2897221
ATGGGGG478250.041.39832726
ACTTTTT750050.040.91860224
TACTTTT746250.040.9159823
TGGGGGG337550.038.8058127
CATGGGT239400.038.2537625
CTTTTTT813600.037.60115425
ATGGGGC161200.035.93517726
ATGGGGA243000.035.66584426
CATGGGA219300.034.71956325
CATGGGC200100.034.49250425