##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18302746
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.24137990004341	35.0	33.0	35.0	32.0	37.0
2	34.905927394719896	35.0	35.0	37.0	33.0	37.0
3	36.889841994201305	39.0	37.0	39.0	33.0	39.0
4	36.94942846281099	39.0	37.0	39.0	33.0	39.0
5	36.955564755146575	39.0	37.0	39.0	33.0	39.0
6	36.97456622082829	39.0	37.0	39.0	33.0	39.0
7	36.725715419970314	39.0	37.0	39.0	32.0	39.0
8	37.7297814218697	40.0	37.0	40.0	33.0	40.0
9	37.742039309292714	40.0	37.0	40.0	33.0	40.0
10	37.29342831944452	39.0	36.0	40.0	32.0	40.0
11	37.492710328821694	39.0	37.0	40.0	33.0	40.0
12	37.566165536034866	39.0	37.0	40.0	33.0	40.0
13	37.57784285483719	40.0	37.0	40.0	33.0	40.0
14	37.60904740742181	40.0	37.0	40.0	33.0	40.0
15	37.457489384379805	39.0	37.0	40.0	32.0	40.0
16	37.348995281910156	39.0	37.0	40.0	32.0	40.0
17	37.28284886868889	39.0	37.0	40.0	32.0	40.0
18	37.2290602732508	39.0	36.0	40.0	32.0	40.0
19	37.111091581558306	39.0	36.0	40.0	32.0	40.0
20	37.05866720764196	39.0	36.0	40.0	32.0	40.0
21	36.84062030910553	39.0	36.0	40.0	31.0	40.0
22	36.829115587355034	39.0	36.0	40.0	31.0	40.0
23	36.281641290328785	38.0	36.0	40.0	30.0	40.0
24	36.3222185348581	38.0	36.0	40.0	30.0	40.0
25	36.422635106229414	38.0	36.0	40.0	30.0	40.0
26	36.28185082172916	38.0	35.0	40.0	30.0	40.0
27	36.281828202172505	38.0	35.0	40.0	30.0	40.0
28	36.203355660401996	38.0	35.0	40.0	30.0	40.0
29	36.147189334321745	38.0	35.0	40.0	30.0	40.0
30	35.99820256479547	38.0	35.0	40.0	30.0	40.0
31	35.855951724402445	38.0	35.0	40.0	29.0	40.0
32	35.83962832680954	38.0	35.0	40.0	29.0	40.0
33	35.66679999820792	38.0	34.0	40.0	29.0	40.0
34	35.51503725178725	38.0	34.0	40.0	27.0	40.0
35	35.43928594102765	38.0	34.0	40.0	27.0	40.0
36	35.36949898119113	38.0	34.0	40.0	27.0	40.0
37	35.29880090124181	38.0	34.0	40.0	27.0	40.0
38	35.17098024525937	38.0	33.0	40.0	27.0	40.0
39	35.11780762296543	38.0	33.0	40.0	27.0	40.0
40	35.06028423275939	38.0	33.0	40.0	27.0	40.0
41	35.00269506007459	37.0	33.0	40.0	27.0	40.0
42	34.77753447488153	37.0	33.0	40.0	26.0	40.0
43	34.78806223940386	37.0	33.0	39.0	26.0	40.0
44	34.618914287506364	37.0	33.0	39.0	26.0	40.0
45	34.40403243316604	37.0	33.0	39.0	26.0	40.0
46	34.22955954259541	37.0	33.0	39.0	25.0	40.0
47	34.0724510955897	36.0	32.0	39.0	24.0	40.0
48	34.0076568838359	36.0	32.0	39.0	24.0	40.0
49	33.77838669672846	36.0	32.0	39.0	24.0	40.0
50	33.50287683607695	36.0	31.0	38.0	23.0	40.0
51	33.295067472389114	36.0	31.0	38.0	23.0	40.0
52	33.010728499428446	35.0	31.0	38.0	22.0	40.0
53	32.370829546560934	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	8.0
12	25.0
13	55.0
14	164.0
15	377.0
16	1104.0
17	2498.0
18	5591.0
19	10833.0
20	19443.0
21	32127.0
22	49712.0
23	72222.0
24	101738.0
25	137712.0
26	182176.0
27	233622.0
28	295923.0
29	370008.0
30	460228.0
31	571087.0
32	713506.0
33	900096.0
34	1163628.0
35	1549564.0
36	2144390.0
37	3034026.0
38	3985781.0
39	2265100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.166770822258037	40.36863648766147	25.016131459181047	18.44846123089945
2	26.157162427976655	42.91026603330451	15.269730563927403	15.662840974791434
3	35.980049113941696	24.475103353343812	18.89831176152475	20.646535771189743
4	21.474100115906104	24.019499587657503	19.329405543845716	35.17699475259068
5	19.978767120518416	36.886557896831434	21.172418608661236	21.962256373988907
6	33.27071795674813	23.02373643823719	22.4667380512192	21.238807553795482
7	21.07016072888735	22.572787711745548	34.727712442712146	21.629339116654954
8	33.33239722607744	24.15057281568569	22.48330933511288	20.033720623123983
9	34.24427678775633	22.896132634960896	21.864527869206075	20.995062708076702
10	20.13757389191764	24.06641058123191	35.956353216069324	19.839662310781126
11	20.535202750450672	37.023624761005806	22.98115812785688	19.460014360686642
12	19.71286166567574	23.40606158223471	36.09508103319578	20.785995718893766
13	20.56933970454488	23.41432810136796	23.276654770819636	32.73967742326753
14	20.52158184351135	36.46969148782374	22.458629978255722	20.550096690409188
15	19.72675575566639	36.92654096822412	22.82768935328065	20.519013922828847
16	20.975852475907168	22.97307190953751	22.634505226702046	33.41657038785328
17	33.19731367085573	23.04901679780728	23.056502013413724	20.69716751792327
18	20.64154198501143	23.048574241264124	22.461066771073586	33.84881700265086
19	20.899699968518384	36.1087838950505	22.571492824082245	20.420023312348867
20	33.88788217899106	23.470489073060403	22.84168178916978	19.79994695877875
21	20.83871458413945	36.02988862982637	22.453526918856877	20.677869867177307
22	34.037018270373196	23.03811133039818	23.113477070599135	19.811393328629485
23	20.697801302602354	22.837665998315227	35.68284234507762	20.7816903540048
24	19.996376500007155	36.35077490557974	23.14932415059467	20.50352444381843
25	20.996679951740575	25.588695816463826	22.34658668158319	31.068037550212413
26	22.130832171303695	30.583470917424084	26.382964610884073	20.90273230038815
27	21.01522908092589	23.157628915355104	33.480943242068705	22.346198761650303
28	28.135963860286317	24.911955834386816	26.132756254170825	20.81932405115604
29	29.69946695430292	23.237977514412318	26.53868441380326	20.523871117481495
30	28.384139735097673	22.9830103089449	27.92818629510566	20.704663660851764
31	27.385737637401515	24.263708844563542	27.24348029525187	21.107073222783075
32	26.319733661823204	24.727120181856865	26.98134476651755	21.971801389802383
33	23.552564189002023	24.786247921486755	29.759053641459047	21.902134248052178
34	23.14134720549583	26.340107653791407	28.258978188300272	22.259566952412495
35	22.30331994991353	24.64693549263045	30.257295817796958	22.792448739659065
36	23.146499437843918	24.696971700312073	28.496669297601574	23.659859564242435
37	23.467888370411742	26.3319941171669	27.95771738295445	22.242400129466912
38	22.987643493495458	26.55584030942679	28.277658445350223	22.178857751727527
39	23.981898672472425	24.490499950116774	27.978074983939567	23.549526393471233
40	24.09668472698031	25.08631218506775	27.925591056118027	22.89141203183391
41	23.324893434023505	25.554684526573222	27.50316264018525	23.617259399218018
42	23.617161053319542	26.685815341588633	27.849487721678486	21.84753588341334
43	24.520495449152822	25.456518928908263	28.51821797669049	21.504767645248425
44	23.706541084053725	26.111895996371253	27.92163536553477	22.259927554040253
45	25.151859726403895	24.260960623067163	28.44592827764752	22.141251372881424
46	22.9895830931599	24.608460391681117	29.187106677872272	23.21484983728671
47	22.421832221241555	26.760022785651945	28.318400965625596	22.499744027480904
48	23.32180100188245	26.35720344914364	27.638901834730156	22.682093714243752
49	23.534626989851688	25.422573202949984	27.593236555869815	23.449563251328517
50	23.34784627399626	25.468926903099675	27.41460762226608	23.768619200637982
51	23.33560220963565	26.152660371290736	27.65373567441738	22.85800174465624
52	24.58478634845285	24.765923102467795	28.038907385809757	22.6103831632696
53	24.47682440656719	24.865771507728947	28.10760199589723	22.549802089806633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6531.0
1	3876.5
2	1222.0
3	1068.5
4	915.0
5	964.0
6	1013.0
7	1147.0
8	1562.5
9	1844.0
10	2604.0
11	3364.0
12	4769.0
13	6174.0
14	8584.0
15	10994.0
16	16064.5
17	21135.0
18	28487.5
19	35840.0
20	49329.0
21	62818.0
22	290944.5
23	519071.0
24	381606.5
25	242625.5
26	241109.0
27	258378.5
28	275648.0
29	293064.0
30	310480.0
31	326309.0
32	342138.0
33	375004.0
34	407870.0
35	455181.5
36	502493.0
37	552793.5
38	603094.0
39	651328.5
40	699563.0
41	757258.0
42	892474.0
43	969995.0
44	1142106.5
45	1314218.0
46	1607885.5
47	1901553.0
48	1825593.0
49	1749633.0
50	1579736.5
51	1409840.0
52	1303383.5
53	1196927.0
54	1143861.5
55	1090796.0
56	1068886.0
57	1046976.0
58	916682.5
59	681836.0
60	577283.0
61	503123.5
62	428964.0
63	366949.0
64	304934.0
65	246859.0
66	188784.0
67	148684.0
68	108584.0
69	83927.5
70	59271.0
71	43966.5
72	28662.0
73	21153.5
74	13645.0
75	10189.5
76	4897.5
77	3061.0
78	2248.5
79	1436.0
80	1111.0
81	786.0
82	568.0
83	350.0
84	240.0
85	130.0
86	86.5
87	43.0
88	35.0
89	27.0
90	25.5
91	24.0
92	15.5
93	4.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8302746E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.83798739587366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20672487416074	42.095931804869245
2	10.206091751382743	10.581265111386175
3	3.18861514518865	4.958741751197433
4	1.4352264554916025	2.9759700363999246
5	0.8765763650815667	2.27199772823095
6	0.5523263075859041	1.717889050262852
7	0.38887160445686103	1.4110824931313553
8	0.3096938663734163	1.2843125393315626
9	0.2339423183792493	1.091438905635449
>10	1.4429902827019825	14.041272951471292
>50	0.10550615505947501	3.7251833194873853
>100	0.048998070796733115	4.509192274431427
>500	0.002172657929501786	0.7642604147224721
>1k	0.00169307181773885	1.6477489673791725
>5k	1.7651365627482148E-4	0.7011193111790965
>10k+	3.945599375554825E-4	6.222593340884129
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	381541	2.0846106917508442	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	159315	0.8704431564531354	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	55505	0.30326050528155724	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	40115	0.21917476208214876	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	29566	0.16153860191252176	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	24966	0.13640576119015146	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCACGCAGAGTAAGCAGTG	24234	0.13240636131867864	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG	23123	0.12633623391812354	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	21219	0.11593342332347288	No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	20474	0.11186299585865421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4040108517049845E-4	0.0	0.0	0.0	0.0
2	2.6771939030350965E-4	0.0	0.0	5.463661026602238E-6	5.463661026602238E-6
3	3.715289498089522E-4	0.0	0.0	5.463661026602238E-6	1.6390983079806712E-5
4	3.879199328887589E-4	0.0	0.0	5.463661026602238E-6	2.731830513301119E-5
5	3.9884725494196334E-4	0.0	0.0	5.463661026602238E-6	2.731830513301119E-5
6	4.043109159685656E-4	0.0	0.0	5.463661026602238E-6	3.2781966159613424E-5
7	4.0977457699516786E-4	0.0	0.0	5.463661026602238E-6	3.2781966159613424E-5
8	4.753385093143947E-4	0.0	0.0	5.463661026602238E-6	3.2781966159613424E-5
9	5.572934247134282E-4	0.0	0.0	2.185464410640895E-5	3.8245627186215666E-5
10	9.288223745223804E-4	5.463661026602238E-6	0.0	2.185464410640895E-5	5.463661026602238E-5
11	0.0015789980366880468	5.463661026602238E-6	0.0	2.185464410640895E-5	7.102759334582909E-5
12	0.002387619868625178	5.463661026602238E-6	0.0	2.731830513301119E-5	8.74185764256358E-5
13	0.003655189226796897	5.463661026602238E-6	0.0	2.731830513301119E-5	8.74185764256358E-5
14	0.005310678517857375	5.463661026602238E-6	0.0	2.731830513301119E-5	1.14736881558647E-4
15	0.007228423538194761	5.463661026602238E-6	0.0	4.9172949239420136E-5	1.2020054258524923E-4
16	0.009747171271458393	5.463661026602238E-6	0.0	5.463661026602238E-5	1.311278646384537E-4
17	0.012779503141222634	5.463661026602238E-6	0.0	5.463661026602238E-5	1.3659152566505595E-4
18	0.01616150931668942	5.463661026602238E-6	0.0	5.463661026602238E-5	1.4205518669165818E-4
19	0.019783916577326702	1.0927322053204475E-5	0.0	6.010027129262461E-5	1.584461697714649E-4
20	0.024471737738151422	1.0927322053204475E-5	0.0	6.556393231922685E-5	1.6390983079806712E-4
21	0.029432741950306256	1.0927322053204475E-5	0.0	7.102759334582909E-5	1.6937349182466938E-4
22	0.03449755572196653	1.0927322053204475E-5	0.0	7.102759334582909E-5	1.8030081387787383E-4
23	0.04029996373221811	1.0927322053204475E-5	0.0	7.102759334582909E-5	2.021554579842828E-4
24	0.04599856218296424	1.0927322053204475E-5	0.0	7.102759334582909E-5	2.1308278003748728E-4
25	0.052724328906711596	1.6390983079806712E-5	0.0	7.649125437243133E-5	2.2401010209069174E-4
26	0.06008934397057141	1.6390983079806712E-5	0.0	7.649125437243133E-5	2.29473763117294E-4
27	0.06725766723747355	1.6390983079806712E-5	0.0	8.195491539903356E-5	2.29473763117294E-4
28	0.07552418637072274	1.6390983079806712E-5	0.0	8.195491539903356E-5	2.29473763117294E-4
29	0.08363772299522705	1.6390983079806712E-5	0.0	8.74185764256358E-5	2.3493742414389622E-4
30	0.09234133501060442	1.6390983079806712E-5	0.0	9.288223745223804E-5	2.4040108517049845E-4
31	0.10160224045069521	1.6390983079806712E-5	0.0	1.0380955950544251E-4	2.4040108517049845E-4
32	0.11142043931549943	1.6390983079806712E-5	0.0	1.0927322053204476E-4	2.4040108517049845E-4
33	0.12133698407878249	2.185464410640895E-5	0.0	1.0927322053204476E-4	2.4040108517049845E-4
34	0.13192555914833765	3.2781966159613424E-5	0.0	1.0927322053204476E-4	2.458647461971007E-4
35	0.142579698150212	3.2781966159613424E-5	0.0	1.14736881558647E-4	2.567920682503052E-4
36	0.15279128060893157	3.2781966159613424E-5	0.0	1.14736881558647E-4	2.622557292769074E-4
37	0.1635492291703114	3.2781966159613424E-5	0.0	1.14736881558647E-4	2.731830513301119E-4
38	0.17447655122351585	3.2781966159613424E-5	0.0	1.2020054258524923E-4	2.786467123567141E-4
39	0.18477008859763447	3.2781966159613424E-5	0.0	1.311278646384537E-4	2.786467123567141E-4
40	0.1955553554641473	3.2781966159613424E-5	0.0	1.4205518669165818E-4	2.895740344099186E-4
41	0.20589806578750533	3.2781966159613424E-5	0.0	1.4205518669165818E-4	3.0050135646312307E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	213240	0.0	44.095013	19
GTACATG	142080	0.0	43.559685	22
GAGTACA	144830	0.0	43.313473	20
ACATGGG	140290	0.0	43.30808	24
GAGTACT	72045	0.0	43.089043	20
TACATGG	142725	0.0	43.05987	23
AGTACAT	144585	0.0	43.011414	21
GTACTTT	71445	0.0	42.799637	22
CATGGGG	87350	0.0	42.673958	25
AGTACTT	73140	0.0	42.28972	21
ATGGGGG	47825	0.0	41.398327	26
ACTTTTT	75005	0.0	40.918602	24
TACTTTT	74625	0.0	40.91598	23
TGGGGGG	33755	0.0	38.80581	27
CATGGGT	23940	0.0	38.25376	25
CTTTTTT	81360	0.0	37.601154	25
ATGGGGC	16120	0.0	35.935177	26
ATGGGGA	24300	0.0	35.665844	26
CATGGGA	21930	0.0	34.719563	25
CATGGGC	20010	0.0	34.492504	25
>>END_MODULE