FastQCFastQC Report
Thu 26 May 2016
SRR805334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23291281
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT484990.2082281348114773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC336450.033.2611119
ACATGGG247900.028.96026824
GTACATG255800.028.70895422
GAGTACA262200.028.52803420
CAACGCA429850.027.29684813
TCAACGC430800.027.24756412
AGTACAT273750.026.99817321
CATGGGG181000.026.99254425
TACATGG270700.026.94643623
AACGCAG437700.026.77507614
GAGTACT144700.025.96855520
ATCAACG460000.025.49239311
GTATCAA460200.025.3638659
ACGCAGA464800.025.23924415
TATCAAC477900.024.4834710
GGTATCA482100.024.2263038
TGGTATC482050.024.1995647
CGCAGAG486900.024.13226716
AGTGGTA484400.024.0142445
GTGGTAT490250.023.7852126
Produced by FastQC (version 0.11.5)