##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23291281
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.1207976066237	35.0	33.0	37.0	32.0	37.0
2	34.65708678711145	35.0	35.0	37.0	32.0	37.0
3	36.51744852505107	38.0	35.0	39.0	32.0	39.0
4	36.64971419991885	39.0	35.0	39.0	32.0	39.0
5	36.66183302670214	39.0	35.0	39.0	32.0	39.0
6	36.59413949795204	39.0	35.0	39.0	32.0	39.0
7	36.53982934644084	38.0	35.0	39.0	32.0	39.0
8	37.38417169927236	40.0	37.0	40.0	32.0	40.0
9	37.36678107142325	40.0	37.0	40.0	32.0	40.0
10	37.28044056486202	39.0	36.0	40.0	32.0	40.0
11	37.2643999701004	39.0	36.0	40.0	32.0	40.0
12	37.179881175277565	39.0	36.0	40.0	32.0	40.0
13	37.15704773816434	39.0	36.0	40.0	32.0	40.0
14	37.13643435927805	39.0	36.0	40.0	32.0	40.0
15	37.05789213568803	39.0	36.0	40.0	31.0	40.0
16	36.902671690749855	39.0	36.0	40.0	31.0	40.0
17	36.851797374305	39.0	36.0	40.0	31.0	40.0
18	36.8037166354225	39.0	36.0	40.0	31.0	40.0
19	36.74225676981872	39.0	36.0	40.0	31.0	40.0
20	36.54679594480012	39.0	36.0	40.0	30.0	40.0
21	36.478659889939074	39.0	36.0	40.0	30.0	40.0
22	36.32260286585353	39.0	36.0	40.0	30.0	40.0
23	36.181709327194156	38.0	35.0	40.0	30.0	40.0
24	36.03462596153471	38.0	35.0	40.0	29.0	40.0
25	36.0184063298193	38.0	35.0	40.0	29.0	40.0
26	35.90085405779098	38.0	35.0	40.0	29.0	40.0
27	35.81435808532815	38.0	35.0	40.0	29.0	40.0
28	35.661138732558335	38.0	34.0	40.0	27.0	40.0
29	35.695668220223695	38.0	34.0	40.0	28.0	40.0
30	35.59848743398871	38.0	34.0	40.0	27.0	40.0
31	35.46511722562619	38.0	34.0	40.0	27.0	40.0
32	35.43037096156283	38.0	34.0	40.0	27.0	40.0
33	35.39656539286096	38.0	34.0	40.0	27.0	40.0
34	35.18624626099354	38.0	34.0	40.0	26.0	40.0
35	35.05822839885878	38.0	34.0	40.0	26.0	40.0
36	34.9896468124703	38.0	33.0	40.0	26.0	40.0
37	34.98323690311409	38.0	33.0	40.0	26.0	40.0
38	34.93880959145184	38.0	33.0	40.0	26.0	40.0
39	34.87442863275747	38.0	33.0	40.0	26.0	40.0
40	34.817910101209115	38.0	33.0	40.0	26.0	40.0
41	34.74598696396304	38.0	33.0	40.0	25.0	40.0
42	34.554872014124086	37.0	33.0	40.0	25.0	40.0
43	34.54786518611836	37.0	33.0	40.0	25.0	40.0
44	34.38326363414704	37.0	33.0	39.0	24.0	40.0
45	34.160301702598495	37.0	33.0	39.0	24.0	40.0
46	34.06080034842223	37.0	32.0	39.0	24.0	40.0
47	33.888349550202925	37.0	32.0	39.0	24.0	40.0
48	33.84130950118201	37.0	32.0	39.0	24.0	40.0
49	33.626052555889906	36.0	31.0	39.0	23.0	40.0
50	33.38243525549325	36.0	31.0	39.0	23.0	40.0
51	33.2223098849737	36.0	31.0	38.0	23.0	40.0
52	33.02895358138524	36.0	31.0	38.0	22.0	40.0
53	32.501707012164765	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	35.0
11	114.0
12	228.0
13	555.0
14	1341.0
15	2840.0
16	5519.0
17	9980.0
18	17684.0
19	30035.0
20	47091.0
21	71583.0
22	102549.0
23	141796.0
24	188251.0
25	243086.0
26	304996.0
27	376120.0
28	459591.0
29	553694.0
30	659916.0
31	783994.0
32	933751.0
33	1126601.0
34	1390221.0
35	1778560.0
36	2399424.0
37	3458496.0
38	4979721.0
39	3223498.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.02851440416695	28.93463867444646	22.346735673319127	25.690111248067467
2	25.04018134511365	23.8520500439628	29.518071590824054	21.589697020099496
3	20.859565431373227	25.37038216146205	27.992178704125376	25.77787370303935
4	21.572196909221095	28.498943445832797	26.844998349382326	23.08386129556378
5	24.9338926442045	28.114585024327344	25.1951105651939	21.756411766274255
6	24.15791986709533	25.897583735304213	27.63307866149569	22.31141773610477
7	23.281488897068392	26.950703140801917	27.405233743906145	22.36257421822355
8	23.04865498810478	25.688690115412715	27.656748463083673	23.605906433398832
9	23.18667659370045	25.565502386923246	28.065515159943327	23.18230585943298
10	22.643031098203657	26.359769563554707	28.154406792825178	22.84279254541646
11	22.599229299582106	27.483331638135315	27.034975019192803	22.882464043089772
12	22.544504958744007	26.066732868836194	28.079992680522807	23.30876949189699
13	22.832612770418255	26.365338170966208	26.77925271692871	24.02279634168683
14	22.93821451898674	27.382628718446185	26.593432108779247	23.08572465378783
15	22.841671954410753	27.20147079930898	26.699415974587225	23.25744127169304
16	23.097475832265303	26.261033044940724	26.49848241494317	24.143008707850804
17	24.192100039495465	26.226724068976715	26.470433292183458	23.110742599344363
18	23.192743241558933	26.03876961511907	26.30612717265315	24.462359970668853
19	23.387970803323356	27.139404655330036	26.222739745400865	23.249884795945743
20	24.31597901377773	26.099594951432685	26.365046216221426	23.219379818568157
21	23.397240366470182	26.950681673541272	26.302919963912675	23.34915799607587
22	24.447702125099944	25.99698144554608	26.34420579958655	23.211110629767422
23	23.244423524837472	26.04678119679205	27.558952210485977	23.1498430678845
24	23.240636700059564	27.04090857003528	26.33098626048091	23.387468469424245
25	23.30333398150149	26.06148197688225	26.339414307010422	24.295769734605837
26	23.26954021979298	26.693705683255466	26.893711857239627	23.143042239711935
27	23.30045736857496	25.910107735164935	27.405890642081904	23.383544254178204
28	24.13724689509349	26.06074779656817	26.5043343901952	23.297670918143147
29	24.076610470673554	26.041500250673206	26.669889904295086	23.211999374358154
30	23.97656015570805	25.90413983670542	26.657885412141997	23.461414595444534
31	23.94115634945111	26.07648329862149	26.56281979509843	23.41954055682897
32	23.82666715497529	26.20044384849421	26.603942479591396	23.368946516939108
33	23.66191451642355	26.083142442873797	26.63060052386127	23.624342516841388
34	23.73872437501398	26.268585227235892	26.490603930286188	23.50208646746394
35	23.6059751286329	26.177572629002242	26.804481041639573	23.411971200725283
36	23.610212765884366	26.08899871157795	26.659899041190567	23.64088948134712
37	23.747255464394595	26.292765949627245	26.405117004942753	23.55486158103541
38	23.71145666054177	26.29034014917428	26.648229438303545	23.349973751980407
39	23.667079539334914	26.091025220982907	26.64296137254108	23.5989338671411
40	23.74788660185758	26.253270483491224	26.439589132087672	23.559253782563527
41	23.698408859521294	26.25621149819969	26.635370549176752	23.410009093102264
42	23.67715197802989	26.077805681877265	26.657593457397212	23.587448882695632
43	23.784011708072217	26.205063602985167	26.44818462325022	23.562740065692395
44	23.76494878061881	26.130134276427302	26.68130190005436	23.423615042899527
45	23.76071114336734	25.87870971974448	26.745626399853233	23.614952737034944
46	23.799206235157268	26.101501244178028	26.507262524547276	23.592029996117432
47	23.749569635092204	26.15520803686152	26.62732461988673	23.467897708159548
48	23.65480885314981	25.93580833961	26.713524258283606	23.69585854895658
49	23.758049203047268	26.169530993164354	26.49184473795151	23.580575065836868
50	23.725487662099823	26.19518007618387	26.60534643843763	23.473985823278674
51	23.68923375232131	25.97876862161424	26.63955666500267	23.69244096106178
52	23.808578841155196	26.094648894579908	26.47554679366927	23.62122547059563
53	23.822395170106788	26.12626587605894	26.48072040348489	23.57061855034938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8668.0
1	5564.5
2	2461.0
3	2225.5
4	1990.0
5	2064.5
6	2139.0
7	2453.5
8	3357.5
9	3947.0
10	5127.0
11	6307.0
12	8699.5
13	11092.0
14	14515.5
15	17939.0
16	25682.0
17	33425.0
18	45855.5
19	58286.0
20	77411.5
21	96537.0
22	150159.5
23	203782.0
24	214988.5
25	265951.0
26	305707.0
27	352067.5
28	398428.0
29	438848.5
30	479269.0
31	517045.5
32	554822.0
33	613742.5
34	672663.0
35	747431.5
36	822200.0
37	899892.5
38	977585.0
39	1052850.5
40	1128116.0
41	1208201.0
42	1357625.5
43	1426965.0
44	1493132.5
45	1559300.0
46	1620177.5
47	1681055.0
48	1686632.5
49	1692210.0
50	1687503.0
51	1682796.0
52	1652462.0
53	1622128.0
54	1557873.0
55	1493618.0
56	1396376.0
57	1299134.0
58	1187962.0
59	951222.0
60	825654.0
61	719099.0
62	612544.0
63	519046.0
64	425548.0
65	345549.5
66	265551.0
67	211150.5
68	156750.0
69	121539.0
70	86328.0
71	64225.5
72	42123.0
73	31454.5
74	20786.0
75	15725.5
76	7883.0
77	5101.0
78	3511.5
79	1922.0
80	1481.5
81	1041.0
82	730.0
83	419.0
84	279.5
85	140.0
86	91.5
87	43.0
88	33.0
89	23.0
90	22.0
91	21.0
92	15.0
93	6.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.3291281E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.24468689002593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69855039782756	50.17939098358375
2	7.768415261165193	9.204746595588617
3	2.7759811531472978	4.933864026924742
4	1.2793576658920611	3.031805773445183
5	0.7675959253853076	2.2737990128756125
6	0.4706522620073386	1.6730187538024337
7	0.34288802271932983	1.4220005481042755
8	0.2534256932525136	1.2011300677306338
9	0.18456986336610245	0.9841305388013646
>10	1.2931783080517096	15.048527008530268
>50	0.11399597512290124	4.635266926187941
>100	0.05094328098497147	4.822882791826621
>500	2.493421722390685E-4	0.09367501918044026
>1k	1.7415051333120147E-4	0.21895673397591314
>5k	1.5132261374413688E-5	0.05940697585937307
>10k+	7.56613068720572E-6	0.2173982435828367
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48499	0.2082281348114773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.611916278885648E-4	0.0	0.0	4.293452129146525E-6	4.293452129146525E-6
2	8.243428087961328E-4	4.293452129146525E-6	0.0	4.293452129146525E-6	4.293452129146525E-6
3	8.458100694418654E-4	4.293452129146525E-6	0.0	4.293452129146525E-6	4.293452129146525E-6
4	8.586904258293049E-4	4.293452129146525E-6	0.0	1.2880356387439574E-5	1.2880356387439574E-5
5	9.660267290579681E-4	4.293452129146525E-6	0.0	1.2880356387439574E-5	1.71738085165861E-5
6	9.917874418328473E-4	4.293452129146525E-6	0.0	2.576071277487915E-5	2.576071277487915E-5
7	9.917874418328473E-4	4.293452129146525E-6	0.0	3.0054164904025673E-5	2.576071277487915E-5
8	9.960808939619937E-4	4.293452129146525E-6	0.0	3.43476170331722E-5	2.576071277487915E-5
9	0.0010046677982202868	4.293452129146525E-6	0.0	3.43476170331722E-5	3.0054164904025673E-5
10	0.001030428510995166	4.293452129146525E-6	0.0	3.8641069162318726E-5	3.43476170331722E-5
11	0.001064776128028338	4.293452129146525E-6	0.0	3.8641069162318726E-5	3.43476170331722E-5
12	0.0010819499365449243	4.293452129146525E-6	0.0	3.8641069162318726E-5	3.8641069162318726E-5
13	0.0012794487344856644	4.293452129146525E-6	0.0	4.7227973420611776E-5	4.293452129146525E-5
14	0.0014039588462309135	4.293452129146525E-6	0.0	4.7227973420611776E-5	4.293452129146525E-5
15	0.001571403479267628	4.293452129146525E-6	0.0	5.15214255497583E-5	4.293452129146525E-5
16	0.0017517284686917821	8.58690425829305E-6	0.0	5.15214255497583E-5	5.5814877678904826E-5
17	0.0019664010751491083	8.58690425829305E-6	0.0	5.5814877678904826E-5	5.5814877678904826E-5
18	0.0022798230805768046	8.58690425829305E-6	0.0	5.5814877678904826E-5	5.5814877678904826E-5
19	0.0025417236604547426	8.58690425829305E-6	0.0	5.5814877678904826E-5	5.5814877678904826E-5
20	0.002764983171170362	8.58690425829305E-6	0.0	6.440178193719788E-5	5.5814877678904826E-5
21	0.0030140033946608603	8.58690425829305E-6	0.0	6.440178193719788E-5	6.440178193719788E-5
22	0.003271610522409652	8.58690425829305E-6	0.0	7.298868619549092E-5	6.440178193719788E-5
23	0.0036537277619036928	8.58690425829305E-6	0.0	7.298868619549092E-5	6.86952340663444E-5
24	0.0040100842886228545	8.58690425829305E-6	0.0	7.298868619549092E-5	6.86952340663444E-5
25	0.0043879080759877485	8.58690425829305E-6	0.0	7.298868619549092E-5	6.86952340663444E-5
26	0.004748558054836057	8.58690425829305E-6	0.0	8.157559045378397E-5	6.86952340663444E-5
27	0.00526377231033364	8.58690425829305E-6	0.0	8.157559045378397E-5	6.86952340663444E-5
28	0.005753225853056343	1.2880356387439574E-5	0.0	9.445594684122355E-5	7.298868619549092E-5
29	0.006246972847908193	1.2880356387439574E-5	0.0	9.874939897037007E-5	7.728213832463745E-5
30	0.006792241268309802	1.2880356387439574E-5	0.0	9.874939897037007E-5	7.728213832463745E-5
31	0.007397618018519462	1.2880356387439574E-5	0.0	1.030428510995166E-4	7.728213832463745E-5
32	0.007990114412341683	1.2880356387439574E-5	0.0	1.0733630322866312E-4	1.030428510995166E-4
33	0.00857402390190561	1.71738085165861E-5	0.0	1.1162975535780965E-4	1.0733630322866312E-4
34	0.009252389338310761	1.71738085165861E-5	0.0	1.1592320748695617E-4	1.1162975535780965E-4
35	0.009926461322586766	1.71738085165861E-5	0.0	1.202166596161027E-4	1.2451011174524922E-4
36	0.010853846982482415	1.71738085165861E-5	0.0	1.202166596161027E-4	1.2451011174524922E-4
37	0.01163954872211623	1.71738085165861E-5	0.0	1.2880356387439575E-4	1.2451011174524922E-4
38	0.012214871307421863	1.71738085165861E-5	0.0	1.373904681326888E-4	1.2880356387439575E-4
39	0.012918997456601893	1.71738085165861E-5	0.0	1.4168392026183533E-4	1.2880356387439575E-4
40	0.01370040574410656	2.1467260645732624E-5	0.0	1.4168392026183533E-4	1.4168392026183533E-4
41	0.014374477728382564	2.1467260645732624E-5	0.0	1.4597737239098184E-4	1.4168392026183533E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	33645	0.0	33.26111	19
ACATGGG	24790	0.0	28.960268	24
GTACATG	25580	0.0	28.708954	22
GAGTACA	26220	0.0	28.528034	20
CAACGCA	42985	0.0	27.296848	13
TCAACGC	43080	0.0	27.247564	12
AGTACAT	27375	0.0	26.998173	21
CATGGGG	18100	0.0	26.992544	25
TACATGG	27070	0.0	26.946436	23
AACGCAG	43770	0.0	26.775076	14
GAGTACT	14470	0.0	25.968555	20
ATCAACG	46000	0.0	25.492393	11
GTATCAA	46020	0.0	25.363865	9
ACGCAGA	46480	0.0	25.239244	15
TATCAAC	47790	0.0	24.48347	10
GGTATCA	48210	0.0	24.226303	8
TGGTATC	48205	0.0	24.199564	7
CGCAGAG	48690	0.0	24.132267	16
AGTGGTA	48440	0.0	24.014244	5
GTGGTAT	49025	0.0	23.785212	6
>>END_MODULE