FastQCFastQC Report
Thu 26 May 2016
SRR805333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17722783
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6252573.527984290051963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG396650.2238079651485887No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG335770.1894567010158619No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG298280.16830313839536376No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA282360.15932035053411195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242680.136931090337223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC197840.11163032352198862No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC177810.10032848678449655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2043700.045.3533812
CAACGCA2048050.045.2708213
ACGCAGA2049600.045.15976715
ATCAACG2052650.045.11326611
GTGGTAT2031600.045.0914086
AAGCAGT2052500.045.0902331
AACGCAG2056150.045.08562514
GGTATCA2043250.045.0056768
AGTGGTA2033450.045.0041585
GTATCAA2045800.044.9622159
TGGTATC2038200.044.911967
CGCAGAG2058400.044.9004816
TATCAAC2048250.044.85795610
AGAGTAC2022100.044.67568619
CAGAGTA2045750.044.3349618
AGCAGTG2090350.044.2771572
CAGTGGT2078600.044.136274
GAGTACT1059650.044.13471620
GCAGTGG2100300.044.039423
GCAGAGT2069950.044.0141817