##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805333_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17722783
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.32580864980404	35.0	33.0	36.0	32.0	37.0
2	34.9676266983577	35.0	35.0	37.0	33.0	37.0
3	36.86083161995495	39.0	37.0	39.0	33.0	39.0
4	36.8800480150324	39.0	37.0	39.0	33.0	39.0
5	36.87319576163631	39.0	37.0	39.0	33.0	39.0
6	36.93212572765801	39.0	37.0	39.0	33.0	39.0
7	36.63353018541162	38.0	35.0	39.0	32.0	39.0
8	37.66028230442138	40.0	37.0	40.0	33.0	40.0
9	37.676460011951846	40.0	37.0	40.0	33.0	40.0
10	37.22961331750211	39.0	36.0	40.0	32.0	40.0
11	37.38961324527869	39.0	37.0	40.0	32.0	40.0
12	37.4493894666543	39.0	37.0	40.0	32.0	40.0
13	37.44136064860694	40.0	37.0	40.0	32.0	40.0
14	37.504138655875884	40.0	37.0	40.0	32.0	40.0
15	37.40044122867159	39.0	37.0	40.0	32.0	40.0
16	37.22945702150729	39.0	36.0	40.0	32.0	40.0
17	37.2354282620286	39.0	36.0	40.0	32.0	40.0
18	37.14393743917081	39.0	36.0	40.0	32.0	40.0
19	37.04167596025974	39.0	36.0	40.0	32.0	40.0
20	36.993652238477445	39.0	36.0	40.0	31.0	40.0
21	36.76834969993144	39.0	36.0	40.0	31.0	40.0
22	36.782246952975726	39.0	36.0	40.0	31.0	40.0
23	36.25753212686743	38.0	35.0	40.0	30.0	40.0
24	36.2442309991608	38.0	35.0	40.0	30.0	40.0
25	36.34655572998891	38.0	35.0	40.0	30.0	40.0
26	36.27646120815224	38.0	35.0	40.0	30.0	40.0
27	36.28042537111694	38.0	35.0	40.0	30.0	40.0
28	36.18280571397844	38.0	35.0	40.0	30.0	40.0
29	36.09537350877681	38.0	35.0	40.0	30.0	40.0
30	35.986302659125265	38.0	35.0	40.0	30.0	40.0
31	35.841034503441136	38.0	35.0	40.0	29.0	40.0
32	35.791295983255	38.0	35.0	40.0	29.0	40.0
33	35.68317504084996	38.0	34.0	40.0	28.0	40.0
34	35.467501181953196	38.0	34.0	40.0	27.0	40.0
35	35.32842861078872	38.0	34.0	40.0	27.0	40.0
36	35.25721609298043	38.0	34.0	40.0	27.0	40.0
37	35.16575241032969	38.0	34.0	40.0	27.0	40.0
38	35.01605910313295	38.0	33.0	40.0	27.0	40.0
39	34.98736660038099	37.0	33.0	40.0	27.0	40.0
40	34.93458877197786	37.0	33.0	40.0	27.0	40.0
41	34.8378571807825	37.0	33.0	40.0	26.0	40.0
42	34.64622029170024	37.0	33.0	40.0	26.0	40.0
43	34.647855136521166	37.0	33.0	39.0	26.0	40.0
44	34.50171093332238	37.0	33.0	39.0	26.0	40.0
45	34.25596233954904	37.0	33.0	39.0	24.0	40.0
46	34.116859017006526	36.0	33.0	39.0	24.0	40.0
47	33.94916170897088	36.0	32.0	39.0	24.0	40.0
48	33.88282918094748	36.0	32.0	39.0	24.0	40.0
49	33.621497199395826	36.0	32.0	39.0	23.0	40.0
50	33.31584187426997	36.0	31.0	39.0	23.0	40.0
51	33.06093563296464	35.0	31.0	38.0	22.0	40.0
52	32.755088012983066	35.0	31.0	38.0	22.0	40.0
53	32.19728413985546	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	4.0
12	23.0
13	53.0
14	165.0
15	466.0
16	1234.0
17	2970.0
18	6460.0
19	12464.0
20	21769.0
21	35289.0
22	54030.0
23	77375.0
24	107663.0
25	143941.0
26	187263.0
27	239895.0
28	300561.0
29	373273.0
30	463324.0
31	571380.0
32	709998.0
33	892503.0
34	1146044.0
35	1508112.0
36	2047115.0
37	2845686.0
38	3649506.0
39	2324214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.993561225683347	42.52785242588594	22.809544076683668	23.669042271747053
2	26.427141832070056	44.70422619291789	14.251791042072796	14.616840932939256
3	35.314555281752305	25.709748858291615	19.258154884591207	19.717540975364873
4	20.428123506336448	24.392664515499625	20.238412894859685	34.94079908330424
5	19.143624339360247	36.214380100461646	23.53115760656777	21.110837953610336
6	31.771516922596188	23.585381596107112	22.280947636722743	22.36215384457396
7	21.688140062426992	23.338682192294517	33.56288907898946	21.410288666289034
8	30.93540670220924	24.59257668504997	23.750711160882577	20.72130545185821
9	31.779196303424804	23.854481545025973	23.700837503906694	20.66548464764253
10	20.657951970635764	24.60728656441824	34.162179833720245	20.572581631225752
11	20.339017861923832	35.7223241970519	23.477148030306527	20.461509910717744
12	20.195383535418788	24.334440025587405	34.63640558031997	20.833770858673834
13	20.261259193886197	24.475253124749088	23.588451091456687	31.67503658990803
14	20.695434797119617	35.71122548868313	23.116533108823823	20.476806605373437
15	20.487098442722004	35.52128917901889	23.079687879719568	20.911924498539534
16	20.699045968119115	24.066524992152758	23.3556377686281	31.87879127110003
17	32.0501921171184	23.999464418201136	23.203246352449273	20.747097112231188
18	20.751898841169584	23.96708801320876	23.202890877804013	32.07812226781764
19	20.90485450281708	35.23734957427397	23.046510246161677	20.811285676747268
20	32.223229274995916	24.222798417156042	23.106754734851744	20.447217572996294
21	21.097262207634092	35.02970159934814	23.13430684108698	20.738729351930786
22	31.88455785978985	23.98062426200219	23.38585875593015	20.748959122277803
23	21.02359431924433	24.076478282220123	34.37474238667821	20.525185011857335
24	20.900588807073923	34.81327396492978	23.313962598312017	20.972174629684286
25	20.660186382691702	24.101434859299466	23.249892525344354	31.988486232664474
26	20.880140551289266	29.312196622844166	29.13061678857096	20.67704603729561
27	21.04151475532934	23.826201562136152	34.31029426924654	20.82198941328797
28	26.22634943958858	23.815627602053244	29.079823411481144	20.87819954687703
29	26.380320743079682	23.97950705597422	28.933401712360862	20.70677048858523
30	26.3052591683823	23.80250325245194	28.98690911015499	20.905328469010765
31	24.613893878856384	24.47751010662377	29.48677417085116	21.421821843668685
32	23.635509163543897	25.318512335224103	29.593162653969184	21.452815847262816
33	23.250970234189516	24.840348155253047	29.85456065224068	22.054120958316762
34	22.589273930623648	24.795784048137364	30.112511110698588	22.502430910540404
35	22.273194903983196	24.98760493766696	30.58837881161215	22.150821346737697
36	22.619077376278884	24.947746637759995	30.376764191041556	22.056411794919565
37	22.986908997305896	24.84425837635094	30.203433625520326	21.96539900082284
38	23.028883217720377	24.991492588946105	30.289069160300613	21.690555033032904
39	22.9550516981447	24.94204775852641	30.282230505220316	21.820670038108574
40	22.581639689432524	25.331072439356735	30.053372543127114	22.03391532808363
41	22.815649212654694	25.574064750440158	29.919465808501972	21.69082022840318
42	22.722458431048896	25.38966368882359	30.0608544380417	21.827023442085817
43	22.54934227880576	25.2567951658608	30.467799554957033	21.72606300037641
44	22.794382800940465	25.109938997729646	30.212230212376916	21.883447988952977
45	23.019212050387345	24.701143155677073	30.07587465241774	22.203770141517843
46	22.585036447153925	24.877988970468127	29.944224899667283	22.592749682710668
47	22.623269720111114	24.997654149463997	30.049716232490127	22.32935989793477
48	22.431984863776755	24.782185732342377	30.12239669130971	22.663432712571158
49	22.235779786955582	25.455787615297215	29.777349302307655	22.531083295439547
50	22.905369884628165	25.406229935783784	29.651223512695495	22.037176666892552
51	22.80053307654898	25.12566451894152	29.74448200375754	22.32932040075196
52	23.099983789227686	25.159643381064928	29.32334047085043	22.417032358856957
53	23.50086890980948	25.443718404722325	28.77354532863151	22.281867356836678
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26203.0
1	15285.0
2	4367.0
3	3703.5
4	3040.0
5	3043.5
6	3047.0
7	3223.0
8	4107.5
9	4816.0
10	6245.5
11	7675.0
12	10353.0
13	13031.0
14	15538.5
15	18046.0
16	24039.0
17	30032.0
18	40300.0
19	50568.0
20	70242.5
21	89917.0
22	461761.5
23	833606.0
24	587211.5
25	319833.5
26	298850.0
27	310004.5
28	321159.0
29	337533.0
30	353907.0
31	373392.0
32	392877.0
33	428067.5
34	463258.0
35	506850.5
36	550443.0
37	600947.5
38	651452.0
39	702930.5
40	754409.0
41	805136.0
42	905954.5
43	956046.0
44	1015826.5
45	1075607.0
46	1118309.0
47	1161011.0
48	1194334.5
49	1227658.0
50	1230403.5
51	1233149.0
52	1216315.5
53	1199482.0
54	1157966.0
55	1116450.0
56	1082266.0
57	1048082.0
58	932979.5
59	713395.5
60	608914.0
61	529790.0
62	450666.0
63	383340.0
64	316014.0
65	257169.0
66	198324.0
67	157118.0
68	115912.0
69	89067.5
70	62223.0
71	46799.0
72	31375.0
73	23838.0
74	16301.0
75	12279.5
76	6230.5
77	4203.0
78	3117.0
79	2031.0
80	1723.0
81	1415.0
82	1020.0
83	625.0
84	412.0
85	199.0
86	142.5
87	86.0
88	66.5
89	47.0
90	36.0
91	25.0
92	18.5
93	8.5
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7722783E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.45813445843729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.813580225956585	21.208779700924946
2	16.467314851561955	11.6780052835221
3	8.408142000594768	8.944110888081697
4	4.655027535956983	6.60234369110763
5	2.7970035262266113	4.95882635568332
6	1.8320687838318477	3.8977044764529207
7	1.2002828526907783	2.979185354520641
8	0.8190774574697712	2.3234366895070484
9	0.6154805120638316	1.9641411677976457
>10	3.118435644842781	20.217895558920514
>50	0.21116382366825875	4.942255356379674
>100	0.05782771392408051	3.732822818889748
>500	0.0031593656198239256	0.72064608629897
>1k	0.0011805265192415914	0.8194146406839601
>5k	7.974346014201066E-5	0.18638107134128634
>10k+	1.754356123124234E-4	4.824050859887883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625257	3.527984290051963	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	39665	0.2238079651485887	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	33577	0.1894567010158619	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	29828	0.16830313839536376	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	28236	0.15932035053411195	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24268	0.136931090337223	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	19784	0.11163032352198862	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	17781	0.10032848678449655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0312836872177467E-4	0.0	0.0	0.0	0.0
2	0.0030469255308266203	0.0	1.692736406014789E-5	0.0	0.0
3	0.003080780258946916	0.0	1.692736406014789E-5	0.0	5.642454686715963E-6
4	0.0037240200932325356	0.0	1.692736406014789E-5	0.0	5.642454686715963E-6
5	0.0037465899119794	0.0	1.692736406014789E-5	0.0	1.692736406014789E-5
6	0.0037465899119794	0.0	1.692736406014789E-5	0.0	2.2569818746863852E-5
7	0.0037465899119794	0.0	1.692736406014789E-5	0.0	2.2569818746863852E-5
8	0.0037465899119794	0.0	1.692736406014789E-5	0.0	3.385472812029578E-5
9	0.0037465899119794	0.0	1.692736406014789E-5	0.0	3.385472812029578E-5
10	0.0037748021854129795	0.0	1.692736406014789E-5	0.0	3.949718280701174E-5
11	0.0038030144588465592	5.642454686715963E-6	1.692736406014789E-5	0.0	7.899436561402348E-5
12	0.003910221097894163	5.642454686715963E-6	1.692736406014789E-5	0.0	7.899436561402348E-5
13	0.004254410833783836	5.642454686715963E-6	1.692736406014789E-5	0.0	7.899436561402348E-5
14	0.00433340519939786	5.642454686715963E-6	1.692736406014789E-5	0.0	9.027927498745541E-5
15	0.004677594935287533	5.642454686715963E-6	1.692736406014789E-5	0.0	9.027927498745541E-5
16	0.004829941211828864	5.642454686715963E-6	1.692736406014789E-5	0.0	1.0156418436088733E-4
17	0.004880723304009308	5.642454686715963E-6	1.692736406014789E-5	0.0	1.1284909373431926E-4
18	0.005140276219598242	5.642454686715963E-6	1.692736406014789E-5	0.0	1.1284909373431926E-4
19	0.005264410222705994	5.642454686715963E-6	1.692736406014789E-5	0.0	1.354189124811831E-4
20	0.005365974407066881	5.642454686715963E-6	1.692736406014789E-5	0.0	1.8620100466162679E-4
21	0.005523963138294928	5.642454686715963E-6	1.692736406014789E-5	0.0	1.8620100466162679E-4
22	0.0056424546867159635	1.1284909373431926E-5	1.692736406014789E-5	0.0	2.313406421553545E-4
23	0.005811728327317442	1.1284909373431926E-5	1.692736406014789E-5	0.0	2.313406421553545E-4
24	0.00594150478511191	1.692736406014789E-5	1.692736406014789E-5	0.0	2.482680062155024E-4
25	0.006082566152279809	1.692736406014789E-5	1.692736406014789E-5	0.0	2.5391046090221834E-4
26	0.006263124702254719	1.692736406014789E-5	1.692736406014789E-5	0.0	2.764802796490822E-4
27	0.006579102164710813	1.692736406014789E-5	1.692736406014789E-5	0.0	2.877651890225141E-4
28	0.006731448441252144	1.692736406014789E-5	1.692736406014789E-5	0.0	2.9905009839594605E-4
29	0.00697407399278093	1.692736406014789E-5	1.692736406014789E-5	0.0	3.1597746245609393E-4
30	0.0071320627240089775	1.692736406014789E-5	1.692736406014789E-5	0.0	3.2726237182952587E-4
31	0.007476252459898651	1.692736406014789E-5	1.692736406014789E-5	0.0	3.2726237182952587E-4
32	0.007724520466114154	1.692736406014789E-5	1.692736406014789E-5	0.0	3.4418973588967375E-4
33	0.00791072147077578	2.2569818746863852E-5	1.692736406014789E-5	5.642454686715963E-6	3.4983219057638975E-4
34	0.008158989476991283	2.2569818746863852E-5	1.692736406014789E-5	5.642454686715963E-6	3.6111709994982163E-4
35	0.008390330119146637	2.2569818746863852E-5	1.692736406014789E-5	5.642454686715963E-6	3.9497182807011745E-4
36	0.008672452853482436	2.2569818746863852E-5	1.692736406014789E-5	1.1284909373431926E-5	4.006142827568334E-4
37	0.009078709590925984	2.2569818746863852E-5	1.692736406014789E-5	1.1284909373431926E-5	4.006142827568334E-4
38	0.009372117234635215	2.2569818746863852E-5	1.692736406014789E-5	1.1284909373431926E-5	4.0625673744354934E-4
39	0.009535748420549979	2.2569818746863852E-5	1.692736406014789E-5	1.1284909373431926E-5	4.1189919213026533E-4
40	0.00968809469709131	2.2569818746863852E-5	1.692736406014789E-5	1.1284909373431926E-5	4.175416468169813E-4
41	0.009896865520499799	2.2569818746863852E-5	1.692736406014789E-5	1.1284909373431926E-5	4.175416468169813E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	204370	0.0	45.35338	12
CAACGCA	204805	0.0	45.27082	13
ACGCAGA	204960	0.0	45.159767	15
ATCAACG	205265	0.0	45.113266	11
GTGGTAT	203160	0.0	45.091408	6
AAGCAGT	205250	0.0	45.090233	1
AACGCAG	205615	0.0	45.085625	14
GGTATCA	204325	0.0	45.005676	8
AGTGGTA	203345	0.0	45.004158	5
GTATCAA	204580	0.0	44.962215	9
TGGTATC	203820	0.0	44.91196	7
CGCAGAG	205840	0.0	44.90048	16
TATCAAC	204825	0.0	44.857956	10
AGAGTAC	202210	0.0	44.675686	19
CAGAGTA	204575	0.0	44.33496	18
AGCAGTG	209035	0.0	44.277157	2
CAGTGGT	207860	0.0	44.13627	4
GAGTACT	105965	0.0	44.134716	20
GCAGTGG	210030	0.0	44.03942	3
GCAGAGT	206995	0.0	44.01418	17
>>END_MODULE