FastQCFastQC Report
Thu 26 May 2016
SRR805332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12535728
Sequences flagged as poor quality0
Sequence length53
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT181700.1449457103727841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG136900.040.6143215
CTCGTAT138350.040.49439611
GCTCGTA139650.040.40350710
GAGCTCG147200.039.1294178
GCCGTCT136650.039.10647618
ATGCCGT138850.039.01152416
TGCCGTC138050.038.94820817
TCGTATG143850.038.86444512
CCGTCTT136650.038.62495419
ATCGGAA152250.038.0630571
CGTATGC151650.037.0514313
AGCTCGT160550.035.23179
GTATGCC160750.034.86625314
CGTCTTC149100.034.78504620
CGGAAGA171700.033.751313
AGAGCTC175750.032.973547
TCGGAAG178300.032.6469462
TGCTTGA153050.030.1715127
TCAACGC126950.029.89562812
GCTTGAA151850.029.8373428
Produced by FastQC (version 0.11.5)