##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12535728
Sequences flagged as poor quality	0
Sequence length	53
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.04966133598304	35.0	33.0	35.0	32.0	37.0
2	34.755905361060805	35.0	35.0	37.0	32.0	37.0
3	36.78668905387864	39.0	35.0	39.0	33.0	39.0
4	36.951486981849	39.0	37.0	39.0	33.0	39.0
5	36.9532876750357	39.0	37.0	39.0	33.0	39.0
6	36.837400029739	39.0	37.0	39.0	33.0	39.0
7	36.7977748081324	39.0	37.0	39.0	33.0	39.0
8	37.63181715493508	40.0	37.0	40.0	33.0	40.0
9	37.5965357576361	40.0	37.0	40.0	33.0	40.0
10	37.567828290467055	40.0	37.0	40.0	33.0	40.0
11	37.558399719585495	40.0	37.0	40.0	33.0	40.0
12	37.474457486633405	40.0	37.0	40.0	32.0	40.0
13	37.5022198152353	40.0	37.0	40.0	32.0	40.0
14	37.508505529156345	40.0	37.0	40.0	32.0	40.0
15	37.428864841355846	39.0	37.0	40.0	32.0	40.0
16	37.300647716670305	39.0	37.0	40.0	32.0	40.0
17	37.22099984939048	39.0	36.0	40.0	32.0	40.0
18	37.20340015354513	39.0	36.0	40.0	32.0	40.0
19	37.13899392201235	39.0	36.0	40.0	32.0	40.0
20	37.00168805513329	39.0	36.0	40.0	32.0	40.0
21	36.97248065688726	39.0	36.0	40.0	31.0	40.0
22	36.77822021983885	39.0	36.0	40.0	31.0	40.0
23	36.665265232302424	39.0	36.0	40.0	31.0	40.0
24	36.53953348381522	39.0	36.0	40.0	30.0	40.0
25	36.538228653333896	39.0	36.0	40.0	30.0	40.0
26	36.40613564684875	38.0	36.0	40.0	30.0	40.0
27	36.339504175585176	38.0	36.0	40.0	30.0	40.0
28	36.18002640133864	38.0	35.0	40.0	30.0	40.0
29	36.21364574917388	38.0	35.0	40.0	30.0	40.0
30	36.12201931950023	38.0	35.0	40.0	30.0	40.0
31	35.9944402909827	38.0	35.0	40.0	30.0	40.0
32	35.96226218373596	38.0	35.0	40.0	30.0	40.0
33	35.888170116645796	38.0	35.0	40.0	29.0	40.0
34	35.699507280311124	38.0	35.0	40.0	29.0	40.0
35	35.6071955294499	38.0	34.0	40.0	28.0	40.0
36	35.559081371261406	38.0	34.0	40.0	28.0	40.0
37	35.50041186279728	38.0	34.0	40.0	27.0	40.0
38	35.440788361074844	38.0	34.0	40.0	27.0	40.0
39	35.32690331187786	38.0	34.0	40.0	27.0	40.0
40	35.28223530376537	38.0	34.0	40.0	27.0	40.0
41	35.25797089726261	38.0	34.0	40.0	27.0	40.0
42	35.06306670023472	38.0	34.0	40.0	27.0	40.0
43	35.0908813592637	38.0	34.0	40.0	27.0	40.0
44	34.9351854954096	38.0	33.0	40.0	26.0	40.0
45	34.70644696502668	37.0	33.0	40.0	26.0	40.0
46	34.64221375894563	37.0	33.0	39.0	26.0	40.0
47	34.48659886366392	37.0	33.0	39.0	25.0	40.0
48	34.45754526581943	37.0	33.0	39.0	25.0	40.0
49	34.25718514313648	37.0	33.0	39.0	25.0	40.0
50	34.04669892326955	37.0	32.0	39.0	24.0	40.0
51	33.91365471554584	37.0	32.0	39.0	24.0	40.0
52	33.74747266373361	36.0	32.0	39.0	24.0	40.0
53	33.25985957895704	36.0	31.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	7.0
12	20.0
13	48.0
14	133.0
15	309.0
16	869.0
17	2218.0
18	4548.0
19	9176.0
20	16493.0
21	27001.0
22	40402.0
23	57227.0
24	77853.0
25	101036.0
26	128384.0
27	161031.0
28	198358.0
29	243798.0
30	296634.0
31	358488.0
32	443761.0
33	556190.0
34	712868.0
35	945165.0
36	1315874.0
37	1948733.0
38	2892720.0
39	1996378.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.630664290099467	29.55996652129019	27.868257830737868	28.941111357872472
2	22.874084377070083	25.660352553916294	32.51390744917248	18.951655619841144
3	15.32094506198603	26.54183307104302	33.70076313078905	24.436458736181894
4	17.569446305790937	29.06221321968696	32.61860818932894	20.749732285193172
5	22.950003382332483	28.803281309230705	29.853511499292264	18.393203809144552
6	20.77603311112047	28.87425445095809	30.56300360058865	19.78670883733278
7	21.679371154192243	29.671025089248904	29.48618540542679	19.163418351132062
8	21.03113596593672	26.888306766068954	30.400380416677837	21.680176851316492
9	20.105709058141656	27.180352030612028	31.65521779030304	21.058721120943275
10	21.796556211174973	27.501809228789902	29.427329629360177	21.274304930674948
11	20.81764218240855	28.18791218188525	28.88811084605537	22.106334789650827
12	20.788860447514494	27.66935434463798	30.185921392040417	21.35586381580711
13	20.972359961862605	27.915905641858213	28.3158664578555	22.79586793842368
14	22.33638126162278	28.256938886995638	28.04098014889921	21.365699702482377
15	21.14632672310695	28.328422569475027	29.08085593433425	21.44439477308378
16	21.320476959934037	28.717247215319286	27.927360899981235	22.034914924765438
17	22.197825287849255	27.375378597876406	28.963216176994266	21.46357993728007
18	22.476476834851553	27.44757225108905	27.76968357960543	22.30626733445397
19	21.713306159801807	28.05718184057599	27.609517373063614	22.619994626558583
20	22.328491811564515	27.35675981482687	27.7044619985373	22.61028637507132
21	22.691294833455224	27.911294820691708	27.782869889965706	21.614540455887365
22	22.277597280349415	27.427820705746008	28.663249553595932	21.63133246030865
23	21.715523821193315	27.36287832665163	28.547899252440708	22.373698599714352
24	21.5052767577599	27.95871927023305	28.788970213776178	21.747033758230874
25	21.518279592537425	27.43287027287127	28.701627859187756	22.34722227540355
26	21.650940416065186	27.589965257701827	28.289007227980694	22.470087098252293
27	21.624839020119136	27.187204444767787	29.518947762746606	21.66900877236647
28	22.899467825083633	27.33325100863707	28.02429982526743	21.742981341011866
29	22.27710269399591	27.232698412090627	28.036808073691454	22.45339082022201
30	22.002846583780375	27.198260842928306	28.968281698518027	21.83061087477329
31	21.868343027225862	27.406330130966467	28.89968576216714	21.82564107964053
32	22.96425863739226	27.41909364976649	28.047673019069975	21.568974693771274
33	21.905931590091935	28.312595806162992	27.967214987434314	21.814257616310755
34	21.875530483750126	28.47217169996031	27.767952527368173	21.884345288921395
35	21.93465748459124	28.366130790329848	27.981972806046844	21.717238919032066
36	21.844682654250317	28.37354160843311	27.89672047766193	21.885055259654646
37	21.891843856216408	28.428360921679218	27.712949738539315	21.966845483565056
38	22.119896028375855	28.388403130635893	27.870978055682126	21.620722785306125
39	21.951042651850774	28.076622275148278	28.09663706806657	21.875698004934378
40	22.078653908253273	27.89392845792442	28.04454595696397	21.982871676858338
41	22.175951807505715	27.847341614304334	28.18406717184674	21.792639406343213
42	22.089032244477544	27.688411873646267	28.302759919487723	21.919795962388463
43	22.096243632599556	27.820123410463278	28.07519435648253	22.008438600454635
44	22.26597450104214	27.63072076867016	28.267763946377904	21.8355407839098
45	22.180770035852724	27.50022176613915	28.380705133359623	21.938303064648498
46	22.185277153428984	27.573843338017547	28.178961764326733	22.06191774422674
47	22.348410878091805	27.48461836440612	28.243409557067604	21.92356120043447
48	22.163714783856193	27.499033163450896	28.277456243466677	22.059795809226237
49	22.256952288690375	27.59657835588009	28.099022250642324	22.047447104787214
50	22.38731567883413	27.579610853075305	28.170162913553966	21.8629105545366
51	22.24519389699585	27.486317507846376	28.243226081484856	22.02526251367292
52	22.299430874696707	27.59774302697059	27.943650340849768	22.15917575748293
53	22.441720177719233	27.538217166166973	28.017551114701917	22.002511541411877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8100.0
1	5000.0
2	1900.0
3	1599.0
4	1298.0
5	1379.5
6	1461.0
7	1770.5
8	2584.5
9	3089.0
10	4393.0
11	5697.0
12	8021.0
13	10345.0
14	13915.5
15	17486.0
16	25504.0
17	33522.0
18	45603.0
19	57684.0
20	76243.5
21	94803.0
22	129651.5
23	164500.0
24	184364.0
25	241114.5
26	278001.0
27	319331.0
28	360661.0
29	395956.0
30	431251.0
31	457730.0
32	484209.0
33	522034.5
34	559860.0
35	610220.5
36	660581.0
37	699141.0
38	737701.0
39	766982.5
40	796264.0
41	816655.5
42	844011.0
43	850975.0
44	840639.5
45	830304.0
46	814933.5
47	799563.0
48	779117.5
49	758672.0
50	731381.5
51	704091.0
52	673209.0
53	642327.0
54	602065.0
55	561803.0
56	514714.5
57	467626.0
58	421845.5
59	326703.5
60	277342.0
61	238527.0
62	199712.0
63	166872.5
64	134033.0
65	107982.5
66	81932.0
67	65262.0
68	48592.0
69	37269.0
70	25946.0
71	19440.0
72	12934.0
73	9516.5
74	6099.0
75	4583.5
76	2323.5
77	1579.0
78	1128.0
79	677.0
80	523.5
81	370.0
82	271.5
83	173.0
84	108.5
85	44.0
86	30.0
87	16.0
88	14.0
89	12.0
90	7.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.2535728E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.536748236830704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75969213111833	45.517995212527396
2	13.869482989456971	16.237488678576977
3	3.9709055718213606	6.973316991898058
4	1.5263112356552178	3.573811861303819
5	0.7542661553598529	2.2076144019930974
6	0.44240680486119976	1.5538233452652448
7	0.30025361460376415	1.2303109171681255
8	0.2225959955123375	1.0424036598265918
9	0.16517807928789294	0.8702088877366785
>10	0.8308387455211884	8.530897367497314
>50	0.07187729321851304	2.9864265891595245
>100	0.08602282455939453	8.98587036687584
>500	8.458412018328039E-5	0.029356580857183522
>1k	5.598373831696532E-5	0.05382603140021853
>5k	1.399558266351811E-5	0.057790311101501
>10k+	1.399558266351811E-5	0.1488587968123438
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18170	0.1449457103727841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002520794963004941	0.0	0.0	0.0	0.0
2	0.0026643845495052223	0.0	0.0	7.977199250015635E-6	0.0
3	0.0026643845495052223	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
4	0.0026643845495052223	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
5	0.0030313357150059416	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
6	0.0030313357150059416	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
7	0.003039312914255957	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
8	0.003063244512006004	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
9	0.003079198910506035	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
10	0.0031111077075060977	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
11	0.003238742895506348	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
12	0.0036854660535072235	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
13	0.006509394588012758	0.0	0.0	7.977199250015635E-6	1.595439850003127E-5
14	0.007594293686014885	0.0	0.0	7.977199250015635E-6	2.3931597750046907E-5
15	0.009086029945767808	0.0	0.0	1.595439850003127E-5	2.3931597750046907E-5
16	0.009572639100018763	0.0	0.0	1.595439850003127E-5	3.190879700006254E-5
17	0.009628479494768872	0.0	0.0	1.595439850003127E-5	3.190879700006254E-5
18	0.010569789006270716	0.0	0.0	1.595439850003127E-5	3.190879700006254E-5
19	0.010657538198020889	0.0	0.0	2.3931597750046907E-5	3.190879700006254E-5
20	0.010777196186771124	0.0	0.0	2.3931597750046907E-5	3.190879700006254E-5
21	0.01111223855527178	0.0	0.0	3.190879700006254E-5	3.190879700006254E-5
22	0.011279759739522109	0.0	0.0	3.190879700006254E-5	4.7863195500093814E-5
23	0.011582893311022703	0.0	0.0	3.190879700006254E-5	4.7863195500093814E-5
24	0.011774346093023077	0.0	0.0	3.190879700006254E-5	4.7863195500093814E-5
25	0.01222904645027397	0.0	0.0	3.190879700006254E-5	4.7863195500093814E-5
26	0.012819359194775126	7.977199250015635E-6	0.0	3.190879700006254E-5	4.7863195500093814E-5
27	0.013968075886777377	7.977199250015635E-6	0.0	3.190879700006254E-5	4.7863195500093814E-5
28	0.014095711074777627	7.977199250015635E-6	0.0	3.190879700006254E-5	4.7863195500093814E-5
29	0.015595424533780567	7.977199250015635E-6	0.0	3.190879700006254E-5	4.7863195500093814E-5
30	0.015978330097781317	7.977199250015635E-6	0.0	3.190879700006254E-5	4.7863195500093814E-5
31	0.016624483237032583	7.977199250015635E-6	0.0	3.190879700006254E-5	4.7863195500093814E-5
32	0.01705525199653343	7.977199250015635E-6	0.0	3.190879700006254E-5	5.5840394750109446E-5
33	0.01734243116953399	7.977199250015635E-6	0.0	3.190879700006254E-5	7.179479325014071E-5
34	0.017701405135784696	7.977199250015635E-6	0.0	3.9885996250078176E-5	7.179479325014071E-5
35	0.018164082692285602	7.977199250015635E-6	0.0	3.9885996250078176E-5	8.774919175017199E-5
36	0.018850121827786948	7.977199250015635E-6	0.0	3.9885996250078176E-5	8.774919175017199E-5
37	0.019121346602287478	7.977199250015635E-6	0.0	3.9885996250078176E-5	9.572639100018763E-5
38	0.019233027391787696	7.977199250015635E-6	0.0	4.7863195500093814E-5	9.572639100018763E-5
39	0.019312799384287854	7.977199250015635E-6	0.0	4.7863195500093814E-5	9.572639100018763E-5
40	0.019352685380537932	7.977199250015635E-6	0.0	4.7863195500093814E-5	9.572639100018763E-5
41	0.019488297767788196	7.977199250015635E-6	0.0	5.5840394750109446E-5	9.572639100018763E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	13690	0.0	40.61432	15
CTCGTAT	13835	0.0	40.494396	11
GCTCGTA	13965	0.0	40.403507	10
GAGCTCG	14720	0.0	39.129417	8
GCCGTCT	13665	0.0	39.106476	18
ATGCCGT	13885	0.0	39.011524	16
TGCCGTC	13805	0.0	38.948208	17
TCGTATG	14385	0.0	38.864445	12
CCGTCTT	13665	0.0	38.624954	19
ATCGGAA	15225	0.0	38.063057	1
CGTATGC	15165	0.0	37.05143	13
AGCTCGT	16055	0.0	35.2317	9
GTATGCC	16075	0.0	34.866253	14
CGTCTTC	14910	0.0	34.785046	20
CGGAAGA	17170	0.0	33.75131	3
AGAGCTC	17575	0.0	32.97354	7
TCGGAAG	17830	0.0	32.646946	2
TGCTTGA	15305	0.0	30.17151	27
TCAACGC	12695	0.0	29.895628	12
GCTTGAA	15185	0.0	29.83734	28
>>END_MODULE