FastQCFastQC Report
Thu 26 May 2016
SRR805331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671296
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4125163.0173876712200514No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG232870.17033498506652187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220770.16148432452929115No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA183960.13455929854784798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG146550.10719539683728593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1358500.045.2113381
TCAACGC1364000.045.12206612
CAACGCA1365850.045.06439213
ACGCAGA1368750.044.91397515
AACGCAG1370200.044.90932514
AGTGGTA1352850.044.839085
GGTATCA1362450.044.8198138
GTATCAA1363900.044.818689
TGGTATC1359800.044.7827267
GTGGTAT1365750.044.5910726
CGCAGAG1377250.044.55317316
ATCAACG1383950.044.4342611
AGAGTAC1359550.044.4331619
TATCAAC1385750.044.32567210
GCAGTGG1385500.044.2454723
GAGTACT711150.044.22751620
CAGTGGT1377250.044.203384
CAGAGTA1379000.043.9342618
AGCAGTG1399150.043.86422
GCAGAGT1383950.043.7788217
Produced by FastQC (version 0.11.5)