##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13671296
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.40544356584775	35.0	35.0	36.0	32.0	37.0
2	34.99797649030494	35.0	35.0	37.0	33.0	37.0
3	36.92659174375275	39.0	37.0	39.0	33.0	39.0
4	36.95780729200801	39.0	37.0	39.0	33.0	39.0
5	36.928197370607734	39.0	37.0	39.0	33.0	39.0
6	36.93021714985909	39.0	37.0	39.0	33.0	39.0
7	36.71280989015233	39.0	37.0	39.0	32.0	39.0
8	37.693732913104945	40.0	37.0	40.0	33.0	40.0
9	37.70290117337815	40.0	37.0	40.0	33.0	40.0
10	37.36682169707978	39.0	37.0	40.0	32.0	40.0
11	37.506513647279675	39.0	37.0	40.0	33.0	40.0
12	37.543943895297126	40.0	37.0	40.0	33.0	40.0
13	37.50567605295065	40.0	37.0	40.0	32.0	40.0
14	37.546403793758834	40.0	37.0	40.0	32.0	40.0
15	37.45041187024259	39.0	37.0	40.0	32.0	40.0
16	37.285411858539234	39.0	37.0	40.0	32.0	40.0
17	37.24858265083281	39.0	37.0	40.0	32.0	40.0
18	37.18399989291432	39.0	36.0	40.0	32.0	40.0
19	37.10322181598584	39.0	36.0	40.0	32.0	40.0
20	37.03124729359967	39.0	36.0	40.0	32.0	40.0
21	36.85680765013061	39.0	36.0	40.0	31.0	40.0
22	36.818543903957604	39.0	36.0	40.0	31.0	40.0
23	36.37239146895803	38.0	36.0	40.0	30.0	40.0
24	36.335348236187706	38.0	36.0	40.0	30.0	40.0
25	36.43093507740598	38.0	36.0	40.0	30.0	40.0
26	36.35417849192937	38.0	35.0	40.0	30.0	40.0
27	36.33803759350979	38.0	35.0	40.0	30.0	40.0
28	36.2332928787439	38.0	35.0	40.0	30.0	40.0
29	36.181505250124054	38.0	35.0	40.0	30.0	40.0
30	36.090581097797894	38.0	35.0	40.0	30.0	40.0
31	35.93490017332665	38.0	35.0	40.0	29.0	40.0
32	35.90159923389853	38.0	35.0	40.0	29.0	40.0
33	35.83898622339828	38.0	35.0	40.0	29.0	40.0
34	35.62819421070248	38.0	34.0	40.0	28.0	40.0
35	35.495409652457234	38.0	34.0	40.0	27.0	40.0
36	35.42060167521792	38.0	34.0	40.0	27.0	40.0
37	35.351969630384716	38.0	34.0	40.0	27.0	40.0
38	35.225803830156266	38.0	34.0	40.0	27.0	40.0
39	35.16880016349584	38.0	33.0	40.0	27.0	40.0
40	35.11774509161385	38.0	33.0	40.0	27.0	40.0
41	35.02957678628273	37.0	33.0	40.0	27.0	40.0
42	34.843789206231804	37.0	33.0	40.0	26.0	40.0
43	34.843568012864324	37.0	33.0	40.0	27.0	40.0
44	34.6860771648862	37.0	33.0	39.0	26.0	40.0
45	34.44982319159793	37.0	33.0	39.0	26.0	40.0
46	34.323324723566806	37.0	33.0	39.0	25.0	40.0
47	34.15371958883781	36.0	33.0	39.0	24.0	40.0
48	34.083004932378024	36.0	33.0	39.0	24.0	40.0
49	33.83367772887077	36.0	32.0	39.0	24.0	40.0
50	33.54286499246304	36.0	32.0	39.0	23.0	40.0
51	33.30416735911504	36.0	31.0	38.0	23.0	40.0
52	33.01041108319211	35.0	31.0	38.0	22.0	40.0
53	32.433759901036446	35.0	30.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	14.0
13	31.0
14	90.0
15	333.0
16	827.0
17	1925.0
18	4334.0
19	8360.0
20	14984.0
21	24913.0
22	37655.0
23	54914.0
24	77261.0
25	103991.0
26	135375.0
27	174628.0
28	218921.0
29	274677.0
30	340583.0
31	421395.0
32	526011.0
33	663258.0
34	859123.0
35	1140403.0
36	1566556.0
37	2230261.0
38	2952321.0
39	1838149.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	12.930939393017313	38.24475016852828	24.974596409879503	23.849714028574905
2	24.225047866712856	39.518689376632615	19.607329107642755	16.64893364901177
3	31.684260219367644	26.160080214779928	21.674997015645044	20.480662550207384
4	20.106352755437378	24.574846452011574	23.247174225472115	32.07162656707894
5	20.432810466542456	34.55704565243851	24.65414398166787	20.355999899351165
6	30.328024497458035	24.742855395713764	23.627299123652946	21.30182098317526
7	21.469427624125757	24.55609914378271	33.506113831490445	20.468359400601084
8	29.44265854532006	25.368619039482432	24.765859798515077	20.422862616682426
9	29.71762881880401	24.70202532371474	25.152319136386193	20.428026721095062
10	20.34433311955209	25.344085886224686	33.90413023022836	20.40745076399487
11	20.28937856367092	34.8225800977464	24.5429548156956	20.345086522887076
12	20.125487737226962	25.085419846077507	34.04751093093149	20.74158148576404
13	20.119877442489724	25.253260554083536	24.702003379928282	29.924858623498462
14	20.574077249150335	34.828709728763094	24.222100084732272	20.37511293735429
15	20.479470271143278	34.6105958059865	24.209021588004532	20.70091233486569
16	20.482578974224534	24.896542361455708	24.524258709635134	30.096619954684616
17	30.251791783310082	24.869624650069753	24.305837573848155	20.57274599277201
18	20.713412978550096	24.815145542895127	24.24901779611823	30.222423682436546
19	20.815400383401837	34.40771818560581	24.139072111378468	20.637809319613883
20	30.41963249131611	25.02046623816791	24.138574718885465	20.42132655163051
21	20.904638448322675	34.317668200586105	24.16108172919378	20.61661162189744
22	30.139242102577548	24.811217605119516	24.490004458977406	20.559535833325533
23	20.876960019006244	24.856114592208375	33.84422369320363	20.42270169558175
24	20.707137055623694	34.0724098139635	24.345914242512194	20.874538887900606
25	20.491085848773956	24.900792141432678	24.455581972623516	30.15254003716985
26	20.731143558006497	29.35915512325971	29.415740833934105	20.493960484799686
27	20.832121548681265	24.688807849672774	33.759740115348244	20.719330486297714
28	25.236766141264148	24.731583604070895	29.38060883181814	20.651041422846816
29	25.49321585897928	24.817976291347946	29.200962366698814	20.487845482973963
30	25.293856559027027	24.671325966462874	29.208686579531307	20.826130894978796
31	23.344824075201064	25.557635501418446	29.801146870055334	21.296393553325156
32	22.530490159820985	26.223212488413683	29.936028010804534	21.3102693409608
33	22.431560255882104	25.787160193152136	30.052842100705007	21.728437450260753
34	21.89389360013857	25.782961615343563	30.347144850056644	21.975999934461225
35	21.758449235536997	25.91978843849186	30.522022198919547	21.7997401270516
36	21.88241699982211	25.939142858146003	30.334168757665697	21.84427138436619
37	22.075295568174372	25.841844109000345	30.303915590738434	21.77894473208685
38	22.274786530845354	25.965131615905324	30.27822673139401	21.481855121855308
39	22.21010356296872	25.786377531435207	30.304230118344304	21.69928878725177
40	21.8529830675892	26.056117869147155	30.208840478620314	21.882058584643328
41	22.10399072626326	26.207522681097682	30.125285854391564	21.56320073824749
42	22.07493715299559	26.068589254449613	30.2213996390686	21.635073953486195
43	21.95344903658	26.008185324931887	30.480321690057764	21.558043948430345
44	22.173223372531766	25.91152294559345	30.24772486822025	21.667528813654535
45	22.13791582012415	25.635777324988062	30.337723651071556	21.88858320381623
46	21.954048833409797	25.752781594371154	30.233190766990926	22.059978805228123
47	22.152991201419383	25.87946307358132	30.165969634480888	21.801576090518413
48	22.063972574363103	25.773072282247416	30.08202002209593	22.08093512129355
49	21.892043007480783	26.1843500426002	29.925802206316064	21.99780474360295
50	22.34400454792289	26.05282630117876	29.86793644143174	21.735232709466608
51	22.230211385957848	25.938674724034943	29.863196583557254	21.96791730644995
52	22.33362513692923	26.124560539103243	29.54043274317226	22.001381580795265
53	22.64508792728941	26.374185739230576	28.964832595241884	22.01589373823813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23825.0
1	13956.5
2	4088.0
3	3078.0
4	2068.0
5	1957.5
6	1847.0
7	2029.5
8	2670.0
9	3128.0
10	4219.5
11	5311.0
12	7294.0
13	9277.0
14	12239.5
15	15202.0
16	21099.0
17	26996.0
18	36152.5
19	45309.0
20	61505.5
21	77702.0
22	324893.5
23	572085.0
24	414305.5
25	265089.0
26	273652.0
27	299024.5
28	324397.0
29	338058.5
30	351720.0
31	359934.0
32	368148.0
33	396854.5
34	425561.0
35	476384.5
36	527208.0
37	571314.0
38	615420.0
39	655856.5
40	696293.0
41	730047.5
42	786199.5
43	808597.0
44	820297.5
45	831998.0
46	837794.0
47	843590.0
48	851438.0
49	859286.0
50	850193.0
51	841100.0
52	826601.0
53	812102.0
54	786438.0
55	760774.0
56	719908.5
57	679043.0
58	615003.5
59	485931.5
60	420899.0
61	370230.0
62	319561.0
63	274641.0
64	229721.0
65	186561.5
66	143402.0
67	113512.5
68	83623.0
69	64958.0
70	46293.0
71	34962.0
72	23631.0
73	18028.0
74	12425.0
75	9435.5
76	4721.5
77	2997.0
78	2204.5
79	1412.0
80	1175.5
81	939.0
82	686.0
83	433.0
84	294.0
85	155.0
86	113.5
87	72.0
88	51.5
89	31.0
90	22.0
91	13.0
92	12.0
93	6.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3671296E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.01415550702332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.42660109593143	24.491228328496966
2	17.25511376692812	13.118771560547687
3	7.42636856546458	8.469213885001208
4	3.5703317777604218	5.428925896458067
5	1.9095400224741685	3.6294775680608926
6	1.1542426790278515	2.632653641604477
7	0.7020584612766902	1.868171166539553
8	0.4994735815534563	1.5189653120658386
9	0.37796655325107775	1.293127139856607
>10	2.373643605985545	17.25280830560622
>50	0.1504424268391418	4.013182103966144
>100	0.15072629324163364	11.053630080999344
>500	0.0025062525358374335	0.6159264236097173
>1k	7.724879019783701E-4	0.5876932843193775
>5k	7.724721027359075E-5	0.23048490103554642
>10k+	1.3518261797878384E-4	3.7957404018322114
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	412516	3.0173876712200514	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	23287	0.17033498506652187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22077	0.16148432452929115	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	18396	0.13455929854784798	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	14655	0.10719539683728593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.755502916475512E-4	0.0	0.0	0.0	1.4629190970629266E-5
2	3.6572977426573163E-4	0.0	0.0	0.0	1.4629190970629266E-5
3	3.6572977426573163E-4	0.0	0.0	0.0	2.925838194125853E-5
4	3.6572977426573163E-4	0.0	0.0	0.0	4.38875729118878E-5
5	3.876735607216755E-4	0.0	0.0	0.0	5.120216839720243E-5
6	4.096173471776194E-4	0.0	0.0	0.0	6.583135936783169E-5
7	4.096173471776194E-4	0.0	0.0	0.0	8.046055033846096E-5
8	4.096173471776194E-4	0.0	0.0	0.0	8.77751458237756E-5
9	4.169319426629341E-4	0.0	0.0	0.0	1.0240433679440486E-4
10	4.169319426629341E-4	0.0	0.0	0.0	1.097189322797195E-4
11	4.169319426629341E-4	0.0	0.0	0.0	1.2434812325034877E-4
12	4.461903246041926E-4	0.0	0.0	0.0	1.3166271873566338E-4
13	8.411784808111828E-4	0.0	0.0	0.0	1.3897731422097802E-4
14	8.77751458237756E-4	0.0	0.0	0.0	1.6092110067692192E-4
15	0.001316627187356634	0.0	0.0	0.0	1.6092110067692192E-4
16	0.001433660715121668	0.0	0.0	2.19437864559439E-5	1.8286488713286581E-4
17	0.001455604501577612	0.0	0.0	2.19437864559439E-5	1.8286488713286581E-4
18	0.0017701321074461412	0.0	0.0	2.19437864559439E-5	2.267524600447536E-4
19	0.0018579072532699168	0.0	0.0	2.19437864559439E-5	2.5601084198601215E-4
20	0.0019017948261818047	0.0	0.0	2.19437864559439E-5	2.7064003295664143E-4
21	0.0019822553765202655	0.0	0.0	2.19437864559439E-5	2.7795462844195604E-4
22	0.0020041991629762093	0.0	0.0	2.19437864559439E-5	2.852692239272707E-4
23	0.002055401331373412	0.0	0.0	2.19437864559439E-5	3.145276058685292E-4
24	0.0020627159268587266	0.0	0.0	2.19437864559439E-5	3.291567968391585E-4
25	0.002113918095255929	0.0	0.0	2.19437864559439E-5	3.364713923244731E-4
26	0.0021578056681678167	0.0	0.0	2.19437864559439E-5	3.364713923244731E-4
27	0.0022967829823887947	0.0	0.0	2.19437864559439E-5	3.58415178780417E-4
28	0.002333355959815368	0.0	0.0	2.19437864559439E-5	3.58415178780417E-4
29	0.002508906251462919	0.0	0.0	2.19437864559439E-5	3.6572977426573163E-4
30	0.002611310588257324	0.0	0.0	2.19437864559439E-5	3.730443697510463E-4
31	0.002691771138595785	0.0	0.0	2.19437864559439E-5	3.730443697510463E-4
32	0.0028161192618461335	0.0	0.0	2.19437864559439E-5	3.949881562069902E-4
33	0.0030575009128615167	0.0	0.0	2.19437864559439E-5	4.023027516923048E-4
34	0.003145276058685292	0.0	0.0	2.19437864559439E-5	4.169319426629341E-4
35	0.0033500847322741017	0.0	0.0	2.19437864559439E-5	4.461903246041926E-4
36	0.0036572977426573166	0.0	0.0	2.19437864559439E-5	4.461903246041926E-4
37	0.004220521595026543	0.0	0.0	2.19437864559439E-5	4.681341110601365E-4
38	0.005010497907440524	0.0	0.0	2.925838194125853E-5	4.7544870654545117E-4
39	0.005054385480352411	0.0	0.0	2.925838194125853E-5	4.900778975160804E-4
40	0.005120216839720243	0.0	0.0	2.925838194125853E-5	4.900778975160804E-4
41	0.00519336279457339	0.0	0.0	2.925838194125853E-5	4.900778975160804E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	135850	0.0	45.211338	1
TCAACGC	136400	0.0	45.122066	12
CAACGCA	136585	0.0	45.064392	13
ACGCAGA	136875	0.0	44.913975	15
AACGCAG	137020	0.0	44.909325	14
AGTGGTA	135285	0.0	44.83908	5
GGTATCA	136245	0.0	44.819813	8
GTATCAA	136390	0.0	44.81868	9
TGGTATC	135980	0.0	44.782726	7
GTGGTAT	136575	0.0	44.591072	6
CGCAGAG	137725	0.0	44.553173	16
ATCAACG	138395	0.0	44.43426	11
AGAGTAC	135955	0.0	44.43316	19
TATCAAC	138575	0.0	44.325672	10
GCAGTGG	138550	0.0	44.245472	3
GAGTACT	71115	0.0	44.227516	20
CAGTGGT	137725	0.0	44.20338	4
CAGAGTA	137900	0.0	43.93426	18
AGCAGTG	139915	0.0	43.8642	2
GCAGAGT	138395	0.0	43.77882	17
>>END_MODULE