FastQCFastQC Report
Thu 26 May 2016
SRR805330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4631551
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1215092.623505603198583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG123820.2673402495190056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC104080.22471953779630194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG76140.16439417378757137No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG66460.1434940476743104No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA46460.10031196892790342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC581200.045.19666312
CAACGCA581350.045.19308513
AGAGTAC566650.045.17109319
ACGCAGA583450.045.0062615
AACGCAG583900.044.9675514
CGCAGAG585600.044.82897616
TGGTATC587600.044.7123877
GGTATCA587200.044.7028278
ATCAACG587650.044.7005911
AGTGGTA586050.044.6903045
AAGCAGT590100.044.658361
GTGGTAT588600.044.6364256
CAGAGTA576000.044.6010418
GAGTACA382100.044.5706620
GTATCAA588500.044.5641449
GTACATG377700.044.411722
TATCAAC592400.044.31043210
ACATGGG378100.044.234224
CAGTGGT595850.044.0657044
GCAGAGT587000.044.03347417
Produced by FastQC (version 0.11.5)