FastQCFastQC Report
Thu 26 May 2016
SRR805329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21882989
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1817840.8307091869396818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT844400.042.0100021
TCAACGC982950.041.14980312
TGGTATC981050.041.1408657
GGTATCA983500.041.0933388
CAACGCA984000.041.05096413
GTGGTAT865500.040.8337946
GTATCAA993950.040.6376579
AGTGGTA868350.040.5915225
AACGCAG1002800.040.4664914
ACGCAGA996450.040.4602315
ATCAACG1007300.040.12940211
TATCAAC1007650.040.0688210
CGCAGAG1008750.039.86671416
AGAGTAC994900.039.4556219
CAGTGGT902500.039.21184
GCAGTGG918200.038.6641773
CAGAGTA1021500.038.60993618
AGCAGTG918800.038.585222
GCAGAGT1036200.038.30032717
GAGTACT391150.037.47743620
Produced by FastQC (version 0.11.5)