##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21882989
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.44819576521288	36.0	35.0	37.0	33.0	37.0
2	35.61086696154717	37.0	35.0	37.0	35.0	37.0
3	37.295774356967414	39.0	37.0	39.0	34.0	39.0
4	37.30896327736581	39.0	37.0	39.0	34.0	39.0
5	37.335997746925706	39.0	37.0	39.0	34.0	39.0
6	32.717599090325365	38.0	35.0	39.0	3.0	39.0
7	34.46953983297254	38.0	35.0	39.0	17.0	39.0
8	36.99626655206928	39.0	36.0	40.0	32.0	40.0
9	37.59300075506138	39.0	37.0	40.0	33.0	40.0
10	37.653716318186696	39.0	37.0	40.0	33.0	40.0
11	37.84197547236349	40.0	37.0	40.0	33.0	40.0
12	37.79567288545454	40.0	37.0	40.0	33.0	40.0
13	37.73020961624575	40.0	37.0	40.0	33.0	40.0
14	37.78146920422982	40.0	37.0	40.0	33.0	40.0
15	37.7699702723426	40.0	37.0	40.0	33.0	40.0
16	37.70508827656039	40.0	37.0	40.0	33.0	40.0
17	37.536220394755034	40.0	37.0	40.0	32.0	40.0
18	37.52827751272918	40.0	37.0	40.0	32.0	40.0
19	37.49382166211389	40.0	37.0	40.0	32.0	40.0
20	37.28229096125762	39.0	37.0	40.0	32.0	40.0
21	37.056988741346075	39.0	37.0	40.0	32.0	40.0
22	37.00013709278929	39.0	36.0	40.0	31.0	40.0
23	36.88723423477478	39.0	36.0	40.0	31.0	40.0
24	36.781187752733416	39.0	36.0	40.0	31.0	40.0
25	36.847954637275556	39.0	36.0	40.0	31.0	40.0
26	36.70045042749873	39.0	36.0	40.0	30.0	40.0
27	36.68614474923878	39.0	36.0	40.0	30.0	40.0
28	36.381413891859104	39.0	36.0	40.0	30.0	40.0
29	36.3928492583897	39.0	35.0	40.0	30.0	40.0
30	36.20978299628081	39.0	35.0	40.0	30.0	40.0
31	36.18824832384644	38.0	35.0	40.0	30.0	40.0
32	36.144710807102264	38.0	35.0	40.0	30.0	40.0
33	36.06690978092618	38.0	35.0	40.0	30.0	40.0
34	35.889675080492886	38.0	35.0	40.0	29.0	40.0
35	35.70034312954231	38.0	35.0	40.0	28.0	40.0
36	35.59023682733652	38.0	34.0	40.0	27.0	40.0
37	35.595536514687275	38.0	34.0	40.0	28.0	40.0
38	35.539305942163566	38.0	34.0	40.0	28.0	40.0
39	35.531178259057754	38.0	34.0	40.0	28.0	40.0
40	35.30806550238635	38.0	34.0	40.0	27.0	40.0
41	35.287958057283674	38.0	34.0	40.0	27.0	40.0
42	34.89772050792513	38.0	33.0	40.0	26.0	40.0
43	35.02328411351849	38.0	33.0	40.0	26.0	40.0
44	34.9648429197675	38.0	33.0	40.0	26.0	40.0
45	30.37366677833636	36.0	28.0	40.0	3.0	40.0
46	31.59111120514661	36.0	28.0	39.0	12.0	40.0
47	32.723119497066875	36.0	29.0	39.0	20.0	40.0
48	33.63867554839058	36.0	31.0	39.0	23.0	40.0
49	33.65755893767529	36.0	31.0	39.0	23.0	40.0
50	34.10636403463896	37.0	32.0	39.0	24.0	40.0
51	34.19561806661787	37.0	32.0	39.0	25.0	40.0
52	34.14383907975277	37.0	32.0	39.0	24.0	40.0
53	33.95150397416002	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	14.0
13	65.0
14	188.0
15	523.0
16	1374.0
17	3263.0
18	7065.0
19	13704.0
20	24409.0
21	39414.0
22	58614.0
23	84775.0
24	115395.0
25	154506.0
26	202840.0
27	262278.0
28	334909.0
29	426392.0
30	541088.0
31	690932.0
32	889638.0
33	1167241.0
34	1548618.0
35	2053730.0
36	2574001.0
37	2867946.0
38	4088863.0
39	3731196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.28697062362002	37.25004842802782	15.679174357762552	21.783806590589613
2	27.55002984281535	29.45073911063978	27.076077221443562	15.923153825101316
3	24.101675506942858	24.718565640187453	27.129625664940015	24.050133187929674
4	20.76727726728739	29.594106179919024	25.050992805416115	24.58762374737747
5	22.217938326432463	31.688107141122266	23.444055106000373	22.649899426444897
6	22.787513168333632	33.443520901098104	25.331790826198375	18.43717510436988
7	21.14697402626305	26.566777509233315	28.992899461769138	23.293349002734498
8	24.367927982781513	24.996265363931773	27.314445023940742	23.321361629345976
9	25.51594300029123	25.492605237794525	26.66604182819815	22.32540993371609
10	21.654390997500386	26.562358551658548	30.224792417525777	21.558458033315283
11	21.68167703232863	30.437108020298325	26.392057319043573	21.489157628329476
12	21.713496268722704	25.699930663036934	30.295715087184842	22.290857981055513
13	22.082568336528432	26.018072759621642	25.9032849671496	25.99607393670033
14	21.976883505265207	29.98240322654277	25.86895236295188	22.17176090524014
15	21.98692326720084	29.614245110665642	25.808604117106672	22.59022750502685
16	22.567666601669455	25.846181250650908	25.39542472922689	26.190727418452752
17	26.29448381114664	25.782666161373108	25.53016409230019	22.39268593518006
18	22.329157136623337	25.70104111463018	25.371017642973726	26.598784105772754
19	22.841349506687592	29.58446855683198	25.21214994898549	22.362031987494944
20	26.526421961826145	25.867490039866126	25.363546085957456	22.242541912350276
21	23.101167760948925	29.162309591253738	25.454694511796355	22.281828136000982
22	26.72097033910678	25.54163418900407	25.55230914753007	22.18508632435907
23	22.456840790807874	25.740487279868397	29.585738950012725	22.216932979311007
24	22.55999397522889	29.30772848261268	25.43313438580077	22.699143156357664
25	22.543606817149158	25.701118800544116	25.394341696191503	26.36093268611523
26	22.697045636681533	27.875849135600262	27.28323356557918	22.143871662139027
27	22.63776214483314	25.236611872354363	29.5563279769505	22.569298005861995
28	25.370830282828365	25.595484236636963	26.755312082823785	22.278373397710887
29	25.22641216883123	25.575386433727132	26.885870115823757	22.31233128161788
30	25.317185874379412	25.17964524864496	26.99872489996682	22.50444397700881
31	24.386357823421655	25.8442665213605	26.87614566730349	22.893229987914356
32	23.68322718619472	26.394684930838284	27.157784523860062	22.76430335910693
33	23.5408837430755	26.008106113840295	27.18593881302047	23.26507133006373
34	23.677268219620274	26.06327682201001	27.249038054170754	23.010416904198966
35	23.4614201926437	26.210889197997588	27.36162322249488	22.966067386863834
36	23.54191193899517	25.95623477213282	27.27973312969266	23.22212015917935
37	23.645549517938342	26.18237846758503	27.061463130105306	23.110608884371324
38	23.54255170534519	26.230923024272418	27.313805257590722	22.91272001279167
39	23.550576203278265	25.991952013502363	27.208760192677516	23.24871159054186
40	23.765076151160155	26.150339882728087	27.004373122885543	23.080210843226215
41	23.412436939030588	26.431480635483574	27.21401998602659	22.942062439459253
42	23.645974505585137	25.89220330001537	27.226216674513704	23.235605519885787
43	23.682221839073264	26.213059833828	27.004642738704483	23.100075588394255
44	23.540920301152642	26.15089739340453	27.22552664080762	23.08265566463521
45	20.321122493823857	36.02244647657594	23.512039420209003	20.144391609391203
46	23.66969612789185	26.10969187070377	26.937522109068375	23.283089892336008
47	23.670377022078657	26.021289870410296	27.237805584968307	23.07052752254274
48	23.501830577166583	25.86759057457827	27.19648124851683	23.43409759973832
49	23.819410593315197	26.257327095489558	26.86978456188046	23.053477749314776
50	23.51306304636903	26.27762596782368	27.181858017659287	23.027452968147998
51	23.51649950562055	26.00122862557761	27.106817994561894	23.37545387423994
52	23.699504670043016	26.198180696430455	26.708636557830378	23.39367807569615
53	23.761580285033272	26.1914448707167	26.85526186573507	23.191712978514957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11271.0
1	7552.0
2	3833.0
3	3301.5
4	2770.0
5	2916.5
6	3063.0
7	3342.0
8	4356.5
9	5092.0
10	6940.0
11	8788.0
12	12351.5
13	15915.0
14	20175.5
15	24436.0
16	32437.5
17	40439.0
18	55426.5
19	70414.0
20	104617.0
21	138820.0
22	262822.5
23	386825.0
24	331590.5
25	312212.5
26	348069.0
27	389409.5
28	430750.0
29	463526.5
30	496303.0
31	525912.5
32	555522.0
33	601133.0
34	646744.0
35	706542.5
36	766341.0
37	837746.0
38	909151.0
39	971896.5
40	1034642.0
41	1104902.0
42	1236338.5
43	1297515.0
44	1352609.0
45	1407703.0
46	1449319.5
47	1490936.0
48	1507595.0
49	1524254.0
50	1529157.0
51	1534060.0
52	1510339.5
53	1486619.0
54	1430284.0
55	1373949.0
56	1285511.5
57	1197074.0
58	1081927.5
59	852637.0
60	738493.0
61	648272.5
62	558052.0
63	479462.0
64	400872.0
65	327186.0
66	253500.0
67	200985.0
68	148470.0
69	113705.5
70	78941.0
71	58380.5
72	37820.0
73	27586.0
74	17352.0
75	12882.5
76	6187.5
77	3962.0
78	2914.5
79	1867.0
80	1426.5
81	986.0
82	779.0
83	572.0
84	408.0
85	244.0
86	174.0
87	104.0
88	83.0
89	62.0
90	49.0
91	36.0
92	25.5
93	11.0
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.1882989E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.38906686049324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.20295046554791	34.45444330741786
2	15.113233478853694	14.626305305729925
3	5.553740601987125	8.06220975946173
4	2.499905912840389	4.838724574455039
5	1.3777813793510654	3.3334777642280646
6	0.8964811901419786	2.602793294937285
7	0.624518578262322	2.115390986740898
8	0.43037273286373023	1.6660267956380992
9	0.30745690443959806	1.3389797435083193
>10	1.8231383206707414	16.445121466288953
>50	0.11617960892409084	3.794377942380347
>100	0.051175647815711975	4.354280836844435
>500	0.002400755153968341	0.7832608025161311
>1k	5.779494948828448E-4	0.4045380506727757
>5k	5.7649101803991117E-5	0.21198530781265573
>10k+	2.8824550901983927E-5	0.9680840613673923
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181784	0.8307091869396818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.037429850190941E-4	0.0	0.0	0.0	0.0
2	7.631498603778488E-4	0.0	0.0	4.56975964298113E-6	0.0
3	7.768591393067921E-4	0.0	0.0	9.13951928596226E-6	4.56975964298113E-6
4	7.997079375216978E-4	4.56975964298113E-6	0.0	9.13951928596226E-6	4.56975964298113E-6
5	9.916378425269053E-4	4.56975964298113E-6	0.0	9.13951928596226E-6	4.56975964298113E-6
6	0.0010190564003847921	4.56975964298113E-6	0.0	1.370927892894339E-5	4.56975964298113E-6
7	0.0010190564003847921	4.56975964298113E-6	0.0	1.370927892894339E-5	4.56975964298113E-6
8	0.0010190564003847921	9.13951928596226E-6	0.0	1.370927892894339E-5	9.13951928596226E-6
9	0.0010190564003847921	1.370927892894339E-5	0.0	1.370927892894339E-5	1.827903857192452E-5
10	0.0010327656793137354	1.370927892894339E-5	0.0	1.370927892894339E-5	3.198831750086791E-5
11	0.0010693237564575846	1.370927892894339E-5	0.0	1.827903857192452E-5	3.198831750086791E-5
12	0.0010830330353865279	1.370927892894339E-5	0.0	2.284879821490565E-5	4.56975964298113E-5
13	0.0011835677475321128	1.370927892894339E-5	0.0	2.284879821490565E-5	5.940687535875469E-5
14	0.0012018467861040372	1.370927892894339E-5	0.0	2.741855785788678E-5	6.854639464471695E-5
15	0.0012795327000347165	1.370927892894339E-5	0.0	2.741855785788678E-5	6.854639464471695E-5
16	0.0013480790946794334	1.370927892894339E-5	0.0	2.741855785788678E-5	7.311615428769808E-5
17	0.0013617883736083767	2.284879821490565E-5	0.0	2.741855785788678E-5	7.311615428769808E-5
18	0.0014074859700381882	2.284879821490565E-5	0.0	2.741855785788678E-5	7.311615428769808E-5
19	0.0014440440471820372	2.284879821490565E-5	0.0	3.655807714384904E-5	8.682543321664147E-5
20	0.0014851718839688673	2.284879821490565E-5	0.0	4.112783678683017E-5	9.13951928596226E-5
21	0.0015217299611127164	2.284879821490565E-5	0.0	4.112783678683017E-5	9.13951928596226E-5
22	0.0016314041925442634	2.741855785788678E-5	0.0	4.112783678683017E-5	1.0053471214558486E-4
23	0.0017090901064749427	3.198831750086791E-5	0.0	4.112783678683017E-5	1.0053471214558486E-4
24	0.001754787702904754	5.483711571577356E-5	0.0	4.112783678683017E-5	1.0053471214558486E-4
25	0.0018096248186205276	5.483711571577356E-5	0.0	5.940687535875469E-5	1.0053471214558486E-4
26	0.0018873107325512069	6.397663500173582E-5	0.0	6.854639464471695E-5	1.0967423143154712E-4
27	0.002010694242911697	6.397663500173582E-5	0.0	6.854639464471695E-5	1.1881375071750939E-4
28	0.0021157987147002633	6.397663500173582E-5	0.0	7.311615428769808E-5	1.2338351036049052E-4
29	0.0022391822250607536	6.854639464471695E-5	0.0	7.311615428769808E-5	1.2338351036049052E-4
30	0.0023260076582773954	7.311615428769808E-5	0.0	7.311615428769808E-5	1.2338351036049052E-4
31	0.0024676702072098104	8.682543321664147E-5	0.0	7.768591393067921E-5	1.2338351036049052E-4
32	0.0026276117947141497	8.682543321664147E-5	0.0	7.768591393067921E-5	1.4166254893241504E-4
33	0.0028515300172202254	8.682543321664147E-5	0.0	7.768591393067921E-5	1.4166254893241504E-4
34	0.0029566344890087914	9.596495250260373E-5	0.0	2.5133678036396215E-4	1.4166254893241504E-4
35	0.0032856571833034327	1.0053471214558486E-4	0.0	2.5133678036396215E-4	1.7365086643328294E-4
36	0.003573552040811244	1.0053471214558486E-4	0.0	3.198831750086791E-4	1.7822062607626409E-4
37	0.0037654819458164514	1.0053471214558486E-4	0.0	3.198831750086791E-4	1.827903857192452E-4
38	0.003856877138676074	1.05104471788566E-4	0.0	3.3816221358060365E-4	1.964996646481886E-4
39	0.0039756908893935836	1.0967423143154712E-4	0.0	3.3816221358060365E-4	2.10208943577132E-4
40	0.004103644159397055	1.2338351036049052E-4	0.0	3.3816221358060365E-4	2.10208943577132E-4
41	0.004249876467972451	1.3252302964645278E-4	0.0	3.3816221358060365E-4	2.1934846286309425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	84440	0.0	42.010002	1
TCAACGC	98295	0.0	41.149803	12
TGGTATC	98105	0.0	41.140865	7
GGTATCA	98350	0.0	41.093338	8
CAACGCA	98400	0.0	41.050964	13
GTGGTAT	86550	0.0	40.833794	6
GTATCAA	99395	0.0	40.637657	9
AGTGGTA	86835	0.0	40.591522	5
AACGCAG	100280	0.0	40.46649	14
ACGCAGA	99645	0.0	40.46023	15
ATCAACG	100730	0.0	40.129402	11
TATCAAC	100765	0.0	40.06882	10
CGCAGAG	100875	0.0	39.866714	16
AGAGTAC	99490	0.0	39.45562	19
CAGTGGT	90250	0.0	39.2118	4
GCAGTGG	91820	0.0	38.664177	3
CAGAGTA	102150	0.0	38.609936	18
AGCAGTG	91880	0.0	38.58522	2
GCAGAGT	103620	0.0	38.300327	17
GAGTACT	39115	0.0	37.477436	20
>>END_MODULE