FastQCFastQC Report
Thu 26 May 2016
SRR805328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20683763
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5103122.4672106328040986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG457710.22128952067377683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG336270.16257679997590382No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG294530.14239671959111114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC221770.10721936815849223No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA211730.10236531911528865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2381550.045.38468612
CAACGCA2378200.045.3399213
AAGCAGT2387600.045.3169251
AGTGGTA2365350.045.22365
GTGGTAT2371650.045.203556
GTATCAA2381600.045.1923079
GGTATCA2370300.045.1826938
AACGCAG2389700.045.11189314
ACGCAGA2390450.045.11150415
TGGTATC2375800.045.0810667
ATCAACG2397350.045.05518311
TATCAAC2397150.044.8687610
CGCAGAG2405050.044.79172516
AGAGTAC2353700.044.582819
CAGTGGT2412800.044.43654
GCAGTGG2425050.044.3951873
AGCAGTG2432700.044.272972
CAGAGTA2380800.044.20463618
GCAGAGT2404500.044.0328117
GTACATG1486950.043.7016722
Produced by FastQC (version 0.11.5)