FastQCFastQC Report
Thu 26 May 2016
SRR805327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22332651
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT929610.4162560011348407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC522900.037.99818812
AAGCAGT527800.037.89031
CAACGCA524750.037.8418313
GGTATCA532250.037.003958
AACGCAG538300.036.99405714
ACGCAGA541550.036.91090415
GTATCAA534800.036.83199
GTGGTAT539300.036.790386
TGGTATC541000.036.5444537
AGTGGTA542300.036.5261885
ATCAACG549300.036.19761711
TATCAAC551750.035.82392510
CGCAGAG558400.035.7339716
AGAGTAC555550.035.03744519
CAGTGGT581800.034.1755754
CAGAGTA578250.033.7839218
GCAGTGG594600.033.7007263
AGCAGTG599300.033.4481932
GCAGAGT598900.032.76031517
GTACATG392600.032.1673522
Produced by FastQC (version 0.11.5)