##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22332651
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.02827371457155	35.0	35.0	37.0	33.0	37.0
2	35.322162514427866	36.0	35.0	37.0	33.0	37.0
3	37.08334523295063	39.0	37.0	39.0	33.0	39.0
4	37.186281377880306	39.0	37.0	39.0	33.0	39.0
5	37.17747095944857	39.0	37.0	39.0	33.0	39.0
6	37.081913786231645	39.0	37.0	39.0	33.0	39.0
7	36.99857428479942	39.0	37.0	39.0	33.0	39.0
8	37.84370964289013	40.0	38.0	40.0	33.0	40.0
9	37.749318162004144	40.0	37.0	40.0	33.0	40.0
10	37.719323782922146	40.0	37.0	40.0	33.0	40.0
11	37.74283062051165	40.0	37.0	40.0	33.0	40.0
12	37.65592195033182	40.0	37.0	40.0	33.0	40.0
13	37.660984403508564	40.0	37.0	40.0	33.0	40.0
14	37.52047076721881	40.0	37.0	40.0	32.0	40.0
15	37.48940150455044	40.0	37.0	40.0	32.0	40.0
16	37.434911153181055	40.0	37.0	40.0	32.0	40.0
17	37.35441435053993	39.0	37.0	40.0	32.0	40.0
18	37.35257941388149	39.0	37.0	40.0	32.0	40.0
19	37.31953143404247	39.0	37.0	40.0	32.0	40.0
20	37.03115492200187	39.0	36.0	40.0	31.0	40.0
21	37.06344893850712	39.0	36.0	40.0	31.0	40.0
22	36.92093500229776	39.0	36.0	40.0	31.0	40.0
23	36.767993732584635	39.0	36.0	40.0	31.0	40.0
24	36.786753171399134	39.0	36.0	40.0	31.0	40.0
25	36.717002741859886	39.0	36.0	40.0	30.0	40.0
26	36.65513431432748	39.0	36.0	40.0	30.0	40.0
27	36.545137610398335	39.0	36.0	40.0	30.0	40.0
28	36.41830228753407	39.0	36.0	40.0	30.0	40.0
29	36.3085166199033	39.0	35.0	40.0	30.0	40.0
30	36.19557718427606	38.0	35.0	40.0	30.0	40.0
31	36.05563320718172	38.0	35.0	40.0	29.0	40.0
32	35.93322476583725	38.0	35.0	40.0	29.0	40.0
33	35.863652595475564	38.0	35.0	40.0	29.0	40.0
34	35.74361807740603	38.0	35.0	40.0	28.0	40.0
35	35.61145826350844	38.0	34.0	40.0	27.0	40.0
36	35.75701469565794	38.0	35.0	40.0	28.0	40.0
37	35.74552000118571	38.0	35.0	40.0	28.0	40.0
38	35.807283470287516	38.0	35.0	40.0	29.0	40.0
39	35.79922450765026	38.0	35.0	40.0	28.0	40.0
40	35.769066198186685	38.0	35.0	40.0	28.0	40.0
41	35.7072098157984	38.0	34.0	40.0	28.0	40.0
42	35.617530359472326	38.0	34.0	40.0	28.0	40.0
43	35.52142896067287	38.0	34.0	40.0	28.0	40.0
44	35.6298844234838	38.0	34.0	40.0	28.0	40.0
45	35.581703309651864	38.0	34.0	40.0	28.0	40.0
46	35.578681232246005	38.0	34.0	40.0	28.0	40.0
47	35.577101303378626	38.0	34.0	40.0	28.0	40.0
48	35.49433405823608	38.0	34.0	40.0	27.0	40.0
49	35.361717110969046	38.0	34.0	40.0	27.0	40.0
50	35.19289425156019	38.0	34.0	40.0	26.0	40.0
51	35.08499282060155	38.0	34.0	40.0	26.0	40.0
52	34.868745228678854	38.0	33.0	40.0	26.0	40.0
53	34.48338341023643	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	7.0
11	23.0
12	88.0
13	191.0
14	506.0
15	1071.0
16	2525.0
17	5174.0
18	9989.0
19	17091.0
20	28381.0
21	43061.0
22	62370.0
23	87633.0
24	118472.0
25	155939.0
26	199705.0
27	253611.0
28	314941.0
29	390489.0
30	478533.0
31	586500.0
32	719901.0
33	895368.0
34	1138022.0
35	1495814.0
36	2060615.0
37	3020152.0
38	4706452.0
39	5540020.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.792775564351942	30.66279950374006	25.43580697159509	23.10861796031291
2	25.60906002605781	26.55769796429452	27.45257157334344	20.38067043630423
3	23.92164727779071	25.541598263457395	26.255015582341745	24.28173887641015
4	22.268954993296585	28.24690628980859	25.40457019634615	24.07956852054868
5	24.510130928925545	29.07381215064884	24.694654476980812	21.721402443444802
6	25.225352780554356	25.73665795431093	26.376246151878703	22.661743113256012
7	23.330620265368406	26.438589847662957	27.77379631285153	22.456993574117107
8	23.790847759184523	25.758943709817522	27.057414724297622	23.392793806700332
9	24.099906455350954	25.581311417081654	27.330767851967057	22.988014275600328
10	22.583418332198896	26.3471855625201	28.443009296119836	22.62638680916117
11	22.440475158994783	28.16721131763533	26.719102895576523	22.67321062779336
12	22.502209880949646	26.085859667981197	28.313557579886062	23.098372871183095
13	22.822516681964895	26.298960208530552	26.297993014801513	24.580530094703043
14	22.91004323669411	27.99277165975504	26.245639176468572	22.851545927082277
15	22.777967559695444	27.88629975008341	26.15255573554613	23.18317695467502
16	23.061391144293616	26.155811058884144	26.00291832796742	24.77987946885482
17	24.941656053282703	26.020605435512334	26.049589903142266	22.988148608062698
18	23.0488355368111	25.914035015368302	25.972003950628164	25.06512549719243
19	23.217928762689212	27.842099892216112	25.929478770791697	23.01049257430298
20	25.07602433763909	25.99949285017708	26.016365007450304	22.90811780473353
21	23.269897514630035	27.626191803203298	25.961123021176487	23.14278766099018
22	25.02590489593018	25.94547776705954	26.0462315915831	22.982385745427177
23	23.134951600685472	25.91772915808338	28.048873373788002	22.898445867443144
24	23.157801552534	27.607918110572722	25.984510302874476	23.249770034018802
25	23.16994968488067	25.972035294869382	25.965753013379377	24.89226200687057
26	23.23073512410148	27.025815251400292	26.77826738975145	22.965182234746784
27	23.270107968821076	25.670758926022707	27.866736465814114	23.1923966393421
28	24.5421065327175	25.87605475050857	26.547614074119547	23.03422464265438
29	24.58415259343819	25.87285763790425	26.559672651491304	22.98331711716625
30	24.541667713340438	25.686632545325676	26.507663599811774	23.264036141522116
31	24.16783838156966	26.04139114518917	26.485525610013788	23.30524486322739
32	23.827887696807693	26.26008887167045	26.619258054048306	23.292765377473547
33	23.785237140006352	26.006016930099342	26.61616392966513	23.59258200022917
34	23.762431965645277	26.175369865404697	26.60067091900554	23.461527249944485
35	23.67651740046446	26.174451926911857	26.686048154336895	23.46298251828679
36	23.65643469734068	26.047476405734365	26.68727953524192	23.608809361683036
37	23.78628493321281	26.178589366752743	26.588854140066044	23.446271559968405
38	23.72879959481747	26.189832993852814	26.70633683390297	23.37503057742675
39	23.781695240748626	26.029135546872606	26.628809092122562	23.56036012025621
40	23.70768253173347	26.206297675990193	26.52831497702624	23.5577048152501
41	23.629720448324743	26.256636527387634	26.69089755622832	23.4227454680593
42	23.72637713274613	26.095128607884483	26.612926517322105	23.565567742047282
43	23.80337202242582	26.17662363505345	26.55231571030237	23.467688632218362
44	23.730111575199917	26.076698194047808	26.71257433790552	23.480615892846757
45	23.730187696928592	25.873502433723612	26.71233253947326	23.68397732987454
46	23.776344330997695	26.12582357553521	26.531570300364248	23.566261793102843
47	23.730626516305655	26.086486552805578	26.656830843772195	23.526056087116572
48	23.723560628785183	25.94031940050467	26.59315725661051	23.74296271409964
49	23.76493502719404	26.21992346542289	26.456411287670235	23.55873021971283
50	23.769918761547835	26.126714647535575	26.637621301653798	23.46574528926279
51	23.79438965844225	25.958946835286145	26.56342948268882	23.68323402358278
52	23.871595002312983	26.121068206367436	26.4266790359998	23.580657755319777
53	23.81906205403022	26.176663934792156	26.435074814897703	23.56919919627992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6765.0
1	4432.0
2	2099.0
3	1998.0
4	1897.0
5	2139.0
6	2381.0
7	2707.0
8	3639.5
9	4246.0
10	5527.0
11	6808.0
12	9555.5
13	12303.0
14	15705.5
15	19108.0
16	27114.5
17	35121.0
18	48301.5
19	61482.0
20	81157.5
21	100833.0
22	180472.0
23	260111.0
24	244896.5
25	267685.0
26	305688.0
27	349791.0
28	393894.0
29	430589.0
30	467284.0
31	499150.0
32	531016.0
33	580228.0
34	629440.0
35	696307.0
36	763174.0
37	836018.5
38	908863.0
39	976755.0
40	1044647.0
41	1119332.0
42	1259358.0
43	1324699.0
44	1385598.5
45	1446498.0
46	1493564.5
47	1540631.0
48	1564655.5
49	1588680.0
50	1605288.0
51	1621896.0
52	1598715.0
53	1575534.0
54	1523536.0
55	1471538.0
56	1377134.5
57	1282731.0
58	1168256.0
59	932875.0
60	811969.0
61	715237.5
62	618506.0
63	526461.5
64	434417.0
65	351625.5
66	268834.0
67	212919.0
68	157004.0
69	119892.5
70	82781.0
71	60560.5
72	38340.0
73	27756.0
74	17172.0
75	12509.5
76	5631.5
77	3416.0
78	2364.5
79	1313.0
80	980.0
81	647.0
82	489.0
83	331.0
84	216.5
85	102.0
86	77.0
87	52.0
88	38.0
89	24.0
90	14.5
91	5.0
92	5.5
93	4.0
94	2.0
95	1.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.2332651E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.42148834995334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50395217627302	32.95980858402846
2	16.658109226749954	15.799046652571466
3	5.741889093805316	8.168667803058389
4	2.5774063944288694	4.888977892260155
5	1.4310650503210414	3.3931617305912334
6	0.8657216612345219	2.463228580352108
7	0.5492656195665194	1.8232895225512529
8	0.38763304843762464	1.4705708872433376
9	0.26130060686259915	1.1152137315753436
>10	1.824731303602596	17.014792495291516
>50	0.1366572167707745	4.364535266272053
>100	0.05904254465941948	4.847386762934541
>500	0.002837436963770856	0.9128970459708662
>1k	3.508266192729176E-4	0.269056779776778
>5k	2.8345278442655564E-5	0.09284666796430911
>10k+	9.4484261475188E-6	0.4165195975584731
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92961	0.4162560011348407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014731793372851256	0.0	0.0	0.0	0.0
2	0.0017284110157813328	8.95549749109499E-6	0.0	0.0	0.0
3	0.001737366513272428	1.3433246236642484E-5	0.0	0.0	0.0
4	0.0017687107544912604	1.3433246236642484E-5	0.0	4.477748745547495E-6	8.95549749109499E-6
5	0.0017731885032368079	1.3433246236642484E-5	0.0	4.477748745547495E-6	8.95549749109499E-6
6	0.0018448324831655677	1.3433246236642484E-5	0.0	4.477748745547495E-6	2.2388743727737473E-5
7	0.0018448324831655677	2.2388743727737473E-5	0.0	4.477748745547495E-6	3.134424121883246E-5
8	0.0018448324831655677	2.2388743727737473E-5	0.0	4.477748745547495E-6	3.134424121883246E-5
9	0.0018627434781477578	2.2388743727737473E-5	0.0	4.477748745547495E-6	3.582198996437996E-5
10	0.0018627434781477578	2.2388743727737473E-5	0.0	4.477748745547495E-6	4.029973870992745E-5
11	0.0018627434781477578	2.2388743727737473E-5	0.0	4.477748745547495E-6	4.029973870992745E-5
12	0.0018761767243844001	2.6866492473284968E-5	0.0	4.477748745547495E-6	5.3732984946569935E-5
13	0.0019119987143487803	3.134424121883246E-5	0.0	4.477748745547495E-6	6.716623118321241E-5
14	0.0019209542118398752	3.582198996437996E-5	0.0	4.477748745547495E-6	8.955497491094989E-5
15	0.0019702094480408975	3.582198996437996E-5	0.0	4.477748745547495E-6	1.0298822114759237E-4
16	0.001974687196786445	3.582198996437996E-5	0.0	8.95549749109499E-6	1.2089921612978235E-4
17	0.001974687196786445	3.582198996437996E-5	0.0	8.95549749109499E-6	1.2537696487532985E-4
18	0.002010509186750825	3.582198996437996E-5	0.0	8.95549749109499E-6	1.2985471362087734E-4
19	0.00201946468424192	3.582198996437996E-5	0.0	1.3433246236642484E-5	1.3881021111197234E-4
20	0.0020239424329874674	4.029973870992745E-5	0.0	1.791099498218998E-5	1.4328795985751983E-4
21	0.0020373756792241102	4.029973870992745E-5	0.0	1.791099498218998E-5	1.522434573486148E-4
22	0.0020552866742063	4.029973870992745E-5	0.0	1.791099498218998E-5	1.5672120609416232E-4
23	0.00207319766918849	4.029973870992745E-5	0.0	2.6866492473284968E-5	1.656767035852573E-4
24	0.0020955864129162277	4.029973870992745E-5	0.0	2.6866492473284968E-5	1.746322010763523E-4
25	0.002117975156643965	4.029973870992745E-5	0.0	3.134424121883246E-5	1.746322010763523E-4
26	0.002149319397862797	4.4777487455474946E-5	0.0	3.134424121883246E-5	1.8806544731299476E-4
27	0.00218066363908163	4.4777487455474946E-5	0.0	3.134424121883246E-5	1.9254319605854227E-4
28	0.0021896191365727248	4.4777487455474946E-5	0.0	3.582198996437996E-5	1.9254319605854227E-4
29	0.00227021861399258	4.4777487455474946E-5	0.0	3.582198996437996E-5	1.9702094480408976E-4
30	0.0022836518602292223	4.4777487455474946E-5	0.0	4.029973870992745E-5	2.0597644229518474E-4
31	0.002346340342666887	4.4777487455474946E-5	0.0	4.029973870992745E-5	2.0597644229518474E-4
32	0.0023866400813768148	4.4777487455474946E-5	0.0	4.029973870992745E-5	2.2836518602292223E-4
33	0.0024403730663233847	4.4777487455474946E-5	0.0	4.029973870992745E-5	2.462761810051122E-4
34	0.0025120170462521445	4.4777487455474946E-5	0.0	4.029973870992745E-5	2.462761810051122E-4
35	0.0025567945337076196	4.925523620102244E-5	0.0	4.029973870992745E-5	2.641871759873022E-4
36	0.0026508272573641167	4.925523620102244E-5	0.0	4.925523620102244E-5	2.6866492473284965E-4
37	0.0027762042222394465	4.925523620102244E-5	0.0	4.925523620102244E-5	2.6866492473284965E-4
38	0.002825459458440469	4.925523620102244E-5	0.0	6.268848243766492E-5	2.776204222239447E-4
39	0.0028971034383692288	6.268848243766492E-5	0.0	6.716623118321241E-5	2.8209817096949214E-4
40	0.002986658413280179	6.716623118321241E-5	0.0	6.716623118321241E-5	2.8209817096949214E-4
41	0.0030717356394455815	6.716623118321241E-5	0.0	6.716623118321241E-5	3.044869146972296E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	52290	0.0	37.998188	12
AAGCAGT	52780	0.0	37.8903	1
CAACGCA	52475	0.0	37.84183	13
GGTATCA	53225	0.0	37.00395	8
AACGCAG	53830	0.0	36.994057	14
ACGCAGA	54155	0.0	36.910904	15
GTATCAA	53480	0.0	36.8319	9
GTGGTAT	53930	0.0	36.79038	6
TGGTATC	54100	0.0	36.544453	7
AGTGGTA	54230	0.0	36.526188	5
ATCAACG	54930	0.0	36.197617	11
TATCAAC	55175	0.0	35.823925	10
CGCAGAG	55840	0.0	35.73397	16
AGAGTAC	55555	0.0	35.037445	19
CAGTGGT	58180	0.0	34.175575	4
CAGAGTA	57825	0.0	33.78392	18
GCAGTGG	59460	0.0	33.700726	3
AGCAGTG	59930	0.0	33.448193	2
GCAGAGT	59890	0.0	32.760315	17
GTACATG	39260	0.0	32.16735	22
>>END_MODULE