FastQCFastQC Report
Thu 26 May 2016
SRR805326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17246095
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT815390.4727968853238951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC494550.040.9224612
CAACGCA497600.040.64328813
GGTATCA503300.040.066268
AACGCAG509050.039.9137614
GTATCAA505750.039.872179
ACGCAGA505750.039.85358415
ATCAACG507300.039.85225711
TGGTATC506500.039.836337
AGTGGTA445900.039.289645
GTGGTAT447200.039.20176
CGCAGAG515800.039.02239216
TATCAAC518450.038.86372810
AAGCAGT456650.038.6374661
AGAGTAC514800.038.08935519
CAGTGGT480850.036.6196334
CAGAGTA537900.036.5715818
GCAGAGT547400.036.2674517
GCAGTGG491950.035.9175723
AGCAGTG498800.035.438452
GTACATG354100.035.20686322
Produced by FastQC (version 0.11.5)