FastQCFastQC Report
Thu 26 May 2016
SRR805325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20326197
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4776472.3499083473411186No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG230510.11340537533902678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG218300.10739834903695955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1591750.043.7106631
TCAACGC1592400.043.67806212
CAACGCA1593550.043.5949313
GTATCAA1593150.043.4318169
AGTGGTA1583850.043.4167985
GTGGTAT1586550.043.4080856
GGTATCA1587300.043.3579648
ACGCAGA1604300.043.28083415
ATCAACG1608600.043.24110811
AACGCAG1606350.043.22999214
TGGTATC1594300.043.154337
CGCAGAG1615250.042.94378316
TATCAAC1615300.042.81588410
CAGTGGT1614900.042.64754
AGAGTAC1582950.042.63536519
GCAGTGG1625400.042.626463
AGCAGTG1641150.042.2374232
CAGAGTA1604700.042.18342618
GCAGAGT1621700.041.8962817
GAGTACT799400.041.7261720