FastQCFastQC Report
Thu 26 May 2016
SRR805324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16291336
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2201711.3514606782402623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252480.1549780816011652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT654700.042.8865131
TCAACGC669000.042.11031312
CAACGCA672950.041.89806413
AGTGGTA666550.041.8631745
TGGTATC668650.041.749277
GGTATCA670150.041.7399868
ACGCAGA677000.041.58146315
AACGCAG678950.041.51741414
GTATCAA676900.041.3272259
GTGGTAT679700.041.1120346
CGCAGAG687600.040.86525716
CAGTGGT687400.040.5592084
ATCAACG696500.040.48140711
GCAGTGG701000.040.1042063
AGAGTAC683100.039.94071219
TATCAAC703550.039.7851610
AGCAGTG712650.039.4123342
GAGTACT369550.039.2609920
GCAGAGT707900.038.90662417
CAGAGTA706200.038.80727818
Produced by FastQC (version 0.11.5)