##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16291336
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.92614025025326	35.0	35.0	37.0	32.0	37.0
2	35.280424331067756	35.0	35.0	37.0	33.0	37.0
3	37.031417988064334	39.0	37.0	39.0	33.0	39.0
4	37.075835953539965	39.0	37.0	39.0	33.0	39.0
5	37.10883987660681	39.0	37.0	39.0	33.0	39.0
6	37.0975052015378	39.0	37.0	39.0	33.0	39.0
7	36.98945783206485	39.0	37.0	39.0	33.0	39.0
8	37.89170028780942	40.0	37.0	40.0	33.0	40.0
9	37.87402659916903	40.0	37.0	40.0	33.0	40.0
10	37.641987679831786	40.0	37.0	40.0	33.0	40.0
11	37.697424262810614	40.0	37.0	40.0	33.0	40.0
12	37.65529094728634	40.0	37.0	40.0	33.0	40.0
13	37.76711517091048	40.0	37.0	40.0	33.0	40.0
14	37.70143903483422	40.0	37.0	40.0	33.0	40.0
15	37.625121291464374	40.0	37.0	40.0	33.0	40.0
16	37.516873201804934	40.0	37.0	40.0	32.0	40.0
17	37.405188500194214	39.0	37.0	40.0	32.0	40.0
18	37.3446625862974	39.0	37.0	40.0	32.0	40.0
19	37.27462652541203	39.0	37.0	40.0	32.0	40.0
20	37.13682579501153	39.0	36.0	40.0	32.0	40.0
21	37.01688517135734	39.0	36.0	40.0	31.0	40.0
22	36.95004719072764	39.0	36.0	40.0	31.0	40.0
23	36.73316289099924	39.0	36.0	40.0	31.0	40.0
24	36.75832540682974	39.0	36.0	40.0	31.0	40.0
25	36.76436462915012	39.0	36.0	40.0	31.0	40.0
26	36.655030011043905	39.0	36.0	40.0	30.0	40.0
27	36.6115908480434	39.0	36.0	40.0	30.0	40.0
28	36.45288133520787	39.0	36.0	40.0	30.0	40.0
29	36.40629749457012	39.0	35.0	40.0	30.0	40.0
30	36.25307157129409	38.0	35.0	40.0	30.0	40.0
31	36.14097124999448	38.0	35.0	40.0	30.0	40.0
32	36.004580901161205	38.0	35.0	40.0	29.0	40.0
33	35.90270061338125	38.0	35.0	40.0	29.0	40.0
34	35.83944023989193	38.0	35.0	40.0	29.0	40.0
35	35.707302765101645	38.0	35.0	40.0	28.0	40.0
36	35.6000808650684	38.0	34.0	40.0	28.0	40.0
37	35.51833158434643	38.0	34.0	40.0	27.0	40.0
38	35.43212705207234	38.0	34.0	40.0	27.0	40.0
39	35.36226636047529	38.0	34.0	40.0	27.0	40.0
40	35.51065412928688	38.0	34.0	40.0	28.0	40.0
41	35.558509443301645	38.0	34.0	40.0	28.0	40.0
42	35.523533367674695	38.0	34.0	40.0	28.0	40.0
43	35.49413332338121	38.0	34.0	40.0	28.0	40.0
44	35.37637539364482	38.0	34.0	40.0	27.0	40.0
45	35.24153850856676	38.0	34.0	40.0	27.0	40.0
46	35.13960512507998	38.0	34.0	40.0	27.0	40.0
47	35.03182575081626	38.0	33.0	40.0	26.0	40.0
48	34.98869079859381	38.0	33.0	40.0	26.0	40.0
49	34.80475088108182	38.0	33.0	40.0	26.0	40.0
50	34.64699033891389	37.0	33.0	40.0	26.0	40.0
51	34.45002889879627	37.0	33.0	40.0	25.0	40.0
52	34.22816213476906	37.0	33.0	40.0	24.0	40.0
53	34.06185729641817	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	18.0
13	48.0
14	156.0
15	440.0
16	1064.0
17	2511.0
18	5201.0
19	10009.0
20	17102.0
21	27412.0
22	41184.0
23	58972.0
24	80525.0
25	108742.0
26	141563.0
27	180137.0
28	228399.0
29	285662.0
30	355448.0
31	443602.0
32	553010.0
33	697884.0
34	902551.0
35	1197856.0
36	1653065.0
37	2406601.0
38	3577772.0
39	3314394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.29437561167482	40.38879929798268	14.792384123683902	21.5244409666586
2	26.202970707865823	30.811991109875827	28.325424016790272	14.659614165468074
3	23.418404727518972	26.21948869018477	27.62246141139069	22.739645170905565
4	20.041720335275144	30.34695865336029	25.513450830551896	24.097870180812674
5	21.07648507157424	32.75363051870024	24.4822585452783	21.687625864447213
6	24.118396428629303	26.543532095833022	28.655335572232993	20.68273590330468
7	20.35581366684721	27.96195474698944	29.364694215379266	22.317537370784077
8	22.8783753523959	26.20810226981998	28.529784174852203	22.383738202931912
9	24.251062036901086	26.535601500085686	27.918913464187344	21.29442299882588
10	20.698738274135405	27.8912238996237	30.874226644149992	20.5358111820909
11	20.70679163452279	31.325196411147623	27.539319058915734	20.428692895413857
12	20.67905296410313	26.928810503939026	31.126213344319954	21.265923187637895
13	20.99605581764442	27.511862747168188	26.78822043815191	24.70386099703548
14	21.11462804523828	30.795411745236855	26.888850613602223	21.20110959592264
15	21.05886220749483	30.53719473958428	26.813276701186446	21.590666351734445
16	21.61796306944992	27.029299500053277	26.304343609388454	25.04839382110835
17	25.307556114489323	26.86104442263053	26.5116071511876	21.319792311692545
18	21.468994316979284	26.819580665453095	26.395944445562968	25.31548057200465
19	21.779208285925723	30.75416282617951	26.018516836188265	21.448112051706502
20	25.455266529399427	27.006311821203617	26.29357715045592	21.24484449894103
21	21.85523028927769	30.225777677165333	26.373987989689734	21.545004043867245
22	25.360829830039723	26.96645014257885	26.32033370375517	21.352386323626252
23	21.654479411633275	26.87064461748257	30.265356997117976	21.20951897376618
24	21.545881811043614	30.298319302971837	26.423057016318367	21.732741869666185
25	21.64649357179792	26.9368393113984	26.238191883096633	25.178475233707044
26	21.599566788138187	28.35965693666867	28.75845786987636	21.28231840531679
27	21.66193122528441	26.384785139782274	30.310264302448857	21.643019332484457
28	23.469493232476452	26.872394013603305	28.22759901336514	21.430513740555103
29	23.581847431051695	26.69907489477843	28.427754482505303	21.291323191664578
30	23.44834088499556	26.433485872490753	28.436292763220894	21.681880479292797
31	22.87031585377651	27.101963890499835	28.27938727677092	21.74833297895274
32	22.44926996779147	27.235685274676065	28.613681529863484	21.701363227668992
33	22.3542071687675	26.927687207482553	28.577779010880384	22.140326612869565
34	22.37834883523365	27.262705771951424	28.35796892286796	22.00097646994697
35	22.215777760645288	27.146011843350355	28.71594447502648	21.922265920977875
36	22.257855341022985	26.974822691030376	28.587968476004672	22.179353491941974
37	22.416080547353516	27.280003309734695	28.277907962858293	22.0260081800535
38	22.338198659704766	27.221555064606118	28.60416113202748	21.836085143661638
39	22.286631372651083	26.986675617027355	28.614135759031672	22.11255725128989
40	22.320127704689167	27.387232084587783	28.300257265579692	21.99238294514336
41	22.21292348276409	27.257832015741375	28.66987704384711	21.859367457647426
42	22.27653398100684	26.994679871558724	28.72154254261283	22.007243604821607
43	22.366821235532804	27.349758178212024	28.340493376356612	21.942927209898563
44	22.301412235313297	27.17161440903312	28.64732517946963	21.879648176183956
45	22.27237839794109	26.858718032701557	28.709671201919846	22.159232367437514
46	22.310981739005324	27.19641900455555	28.44810885982586	22.044490396613266
47	22.280898263960673	27.120795986283753	28.65161580363943	21.946689946116145
48	22.300331906480846	26.93794419315887	28.518041737031268	22.243682163329023
49	22.317776761832178	27.413012658998625	28.214340432239567	22.05487014692963
50	22.343858109611144	27.206872413655947	28.51248049883693	21.936788977895983
51	22.303045005025986	27.05284575801518	28.443904170904094	22.200205066054743
52	22.50116258114129	27.448589851685583	27.907005294102337	22.14324227307079
53	22.499781478940708	27.263147724655607	28.15169363642123	22.085377159982457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29366.0
1	17976.5
2	6587.0
3	5272.5
4	3958.0
5	3874.0
6	3790.0
7	4134.5
8	5259.5
9	6040.0
10	7495.5
11	8951.0
12	11968.0
13	14985.0
14	19061.0
15	23137.0
16	31982.0
17	40827.0
18	54889.5
19	68952.0
20	88563.5
21	108175.0
22	254996.0
23	401817.0
24	326775.5
25	295173.5
26	338613.0
27	386385.5
28	434158.0
29	462929.5
30	491701.0
31	507194.0
32	522687.0
33	561943.5
34	601200.0
35	665858.5
36	730517.0
37	785560.0
38	840603.0
39	886584.0
40	932565.0
41	965097.5
42	1011351.5
43	1025073.0
44	1025949.0
45	1026825.0
46	1023382.0
47	1019939.0
48	1008307.0
49	996675.0
50	977439.0
51	958203.0
52	932571.5
53	906940.0
54	864120.0
55	821300.0
56	768590.5
57	715881.0
58	653374.5
59	523016.0
60	455164.0
61	395234.0
62	335304.0
63	286135.5
64	236967.0
65	194096.5
66	151226.0
67	120808.0
68	90390.0
69	69822.5
70	49255.0
71	36990.5
72	24726.0
73	18435.5
74	12145.0
75	9158.5
76	4545.5
77	2919.0
78	2205.5
79	1492.0
80	1136.5
81	781.0
82	593.0
83	405.0
84	265.0
85	125.0
86	89.0
87	53.0
88	33.5
89	14.0
90	11.0
91	8.0
92	7.5
93	5.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6291336E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.96488461641356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.54886703275095	25.15139156051362
2	16.53982493466911	12.889447403101236
3	7.600699032711194	8.884810824410337
4	3.778543450889222	5.889220383280146
5	2.064468824359907	4.022089476768337
6	1.2058414894786729	2.8191284701932458
7	0.8133095084404648	2.2183357807670325
8	0.5594074985597813	1.7437798907950737
9	0.4219970974877792	1.479876139086547
>10	2.18422999420731	14.95157261610024
>50	0.12831816719839184	3.475178688014554
>100	0.14397475290346426	11.971257935625374
>500	0.009872036626161792	2.4341301786440903
>1k	5.516381501435175E-4	0.3743663784715154
>5k	6.302771156063758E-5	0.18862290399778017
>10k+	3.151385578031879E-5	1.5067913702308189
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	220171	1.3514606782402623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25248	0.1549780816011652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1662640804903907E-4	0.0	0.0	3.069116001290502E-5	2.4552928010324014E-5
2	1.289028720542011E-4	0.0	0.0	4.296762401806703E-5	2.4552928010324014E-5
3	1.289028720542011E-4	0.0	0.0	5.5244088023229034E-5	3.069116001290502E-5
4	1.289028720542011E-4	0.0	0.0	7.365878403097204E-5	3.069116001290502E-5
5	1.289028720542011E-4	0.0	0.0	8.593524803613405E-5	4.296762401806703E-5
6	1.534558000645251E-4	0.0	0.0	8.593524803613405E-5	4.910585602064803E-5
7	1.534558000645251E-4	0.0	0.0	9.207348003871506E-5	5.5244088023229034E-5
8	1.534558000645251E-4	0.0	0.0	9.207348003871506E-5	6.138232002581004E-5
9	1.595940320671061E-4	0.0	0.0	9.207348003871506E-5	6.752055202839105E-5
10	1.657322640696871E-4	0.0	0.0	1.1662640804903907E-4	7.365878403097204E-5
11	1.657322640696871E-4	0.0	0.0	1.350411040567821E-4	7.365878403097204E-5
12	1.7800872807484912E-4	0.0	0.0	1.4117933605936307E-4	7.365878403097204E-5
13	2.2711458409549714E-4	0.0	0.0	1.657322640696871E-4	7.365878403097204E-5
14	2.700822081135642E-4	0.0	0.0	1.7800872807484912E-4	7.365878403097204E-5
15	2.884969041213072E-4	0.0	0.0	1.7800872807484912E-4	7.365878403097204E-5
16	3.069116001290502E-4	0.0	0.0	1.7800872807484912E-4	8.593524803613405E-5
17	3.191880641342122E-4	0.0	0.0	1.7800872807484912E-4	9.821171204129605E-5
18	3.437409921445362E-4	0.0	0.0	1.964234240825921E-4	1.0434994404387706E-4
19	3.744321521574412E-4	6.138232002581003E-6	0.0	2.0256165608517312E-4	1.1048817604645807E-4
20	3.8670861616260324E-4	6.138232002581003E-6	0.0	2.0256165608517312E-4	1.1048817604645807E-4
21	4.1739977617550824E-4	6.138232002581003E-6	0.0	2.0869988808775412E-4	1.1048817604645807E-4
22	4.2967624018067026E-4	6.138232002581003E-6	0.0	2.1483812009033513E-4	1.1662640804903907E-4
23	4.542291681909943E-4	6.138232002581003E-6	0.0	2.2097635209291614E-4	1.2276464005162008E-4
24	4.849203282038993E-4	6.138232002581003E-6	0.0	2.3325281609807815E-4	1.2276464005162008E-4
25	4.971967922090614E-4	6.138232002581003E-6	0.0	2.3939104810065916E-4	1.4117933605936307E-4
26	5.401644162271284E-4	6.138232002581003E-6	0.0	2.4552928010324016E-4	1.4731756806194408E-4
27	6.260996642632624E-4	6.138232002581003E-6	0.0	2.5166751210582114E-4	1.534558000645251E-4
28	6.874819842890724E-4	6.138232002581003E-6	0.0	2.6394397611098316E-4	1.657322640696871E-4
29	7.181731443019775E-4	6.138232002581003E-6	0.0	2.6394397611098316E-4	1.657322640696871E-4
30	7.856936963303684E-4	6.138232002581003E-6	0.0	2.7622044011614517E-4	1.718704960722681E-4
31	9.391494963948936E-4	1.2276464005162007E-5	0.0	3.069116001290502E-4	1.718704960722681E-4
32	0.0011785405444955527	1.2276464005162007E-5	0.0	3.069116001290502E-4	1.718704960722681E-4
33	0.001608216784676223	1.2276464005162007E-5	0.0	3.376027601419552E-4	1.718704960722681E-4
34	0.0020992753448827034	1.2276464005162007E-5	0.0	3.376027601419552E-4	1.7800872807484912E-4
35	0.0025841956730866026	1.2276464005162007E-5	0.0	3.437409921445362E-4	1.7800872807484912E-4
36	0.003099807161303407	1.2276464005162007E-5	0.0	3.437409921445362E-4	1.9028519208001113E-4
37	0.003283954121380837	1.2276464005162007E-5	0.0	3.5601745614969823E-4	2.0869988808775412E-4
38	0.003320783513396323	1.2276464005162007E-5	0.0	3.5601745614969823E-4	2.0869988808775412E-4
39	0.0034189952254376193	1.2276464005162007E-5	0.0	3.621556881522792E-4	2.1483812009033513E-4
40	0.003468101081458267	1.2276464005162007E-5	0.0	3.6829392015486024E-4	2.2097635209291614E-4
41	0.0035601745614969823	1.2276464005162007E-5	0.0	3.8670861616260324E-4	2.2097635209291614E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	65470	0.0	42.886513	1
TCAACGC	66900	0.0	42.110313	12
CAACGCA	67295	0.0	41.898064	13
AGTGGTA	66655	0.0	41.863174	5
TGGTATC	66865	0.0	41.74927	7
GGTATCA	67015	0.0	41.739986	8
ACGCAGA	67700	0.0	41.581463	15
AACGCAG	67895	0.0	41.517414	14
GTATCAA	67690	0.0	41.327225	9
GTGGTAT	67970	0.0	41.112034	6
CGCAGAG	68760	0.0	40.865257	16
CAGTGGT	68740	0.0	40.559208	4
ATCAACG	69650	0.0	40.481407	11
GCAGTGG	70100	0.0	40.104206	3
AGAGTAC	68310	0.0	39.940712	19
TATCAAC	70355	0.0	39.78516	10
AGCAGTG	71265	0.0	39.412334	2
GAGTACT	36955	0.0	39.26099	20
GCAGAGT	70790	0.0	38.906624	17
CAGAGTA	70620	0.0	38.807278	18
>>END_MODULE