FastQCFastQC Report
Thu 26 May 2016
SRR805323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18135613
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4534222.5001746563515663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281270.1550926345858836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG242690.1338195736752874No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG210220.1159155745107706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1356850.044.47654312
CAACGCA1357750.044.4522613
AACGCAG1365800.044.14895614
ATCAACG1373800.044.1279311
ACGCAGA1364900.044.0988715
GGTATCA1370100.044.0326968
GTATCAA1370050.044.003439
GTGGTAT1368100.043.9338846
TGGTATC1372250.043.8575337
AGTGGTA1369200.043.841955
CGCAGAG1376950.043.7129516
AAGCAGT1392450.043.690471
AGAGTAC1354300.043.44107419
TATCAAC1389600.043.38604710
CAGTGGT1406600.042.8616874
GAGTACT730100.042.7383920
CAGAGTA1375950.042.66190718
GCAGTGG1424250.042.6341253
GCAGAGT1385300.042.49949317
AGCAGTG1432150.042.462942