##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18135613
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.82432846355952	35.0	33.0	35.0	32.0	35.0
2	34.63382428815613	35.0	35.0	35.0	32.0	37.0
3	36.93654231593936	39.0	37.0	39.0	33.0	39.0
4	37.00232967035633	39.0	37.0	39.0	33.0	39.0
5	37.03276305024815	39.0	37.0	39.0	33.0	39.0
6	37.01506670880107	39.0	37.0	39.0	33.0	39.0
7	36.8888619866337	39.0	37.0	39.0	33.0	39.0
8	37.759271881242725	40.0	37.0	40.0	33.0	40.0
9	37.758961662889476	40.0	37.0	40.0	33.0	40.0
10	37.479025881286724	39.0	37.0	40.0	32.0	40.0
11	37.59303211862758	40.0	37.0	40.0	33.0	40.0
12	37.53768576777637	40.0	37.0	40.0	33.0	40.0
13	37.640256990486066	40.0	37.0	40.0	33.0	40.0
14	37.56657026150702	40.0	37.0	40.0	33.0	40.0
15	37.49700294111922	40.0	37.0	40.0	32.0	40.0
16	37.38514832666533	39.0	37.0	40.0	32.0	40.0
17	37.25403673975619	39.0	37.0	40.0	32.0	40.0
18	37.08354479112451	39.0	36.0	40.0	32.0	40.0
19	37.070569271631456	39.0	36.0	40.0	31.0	40.0
20	36.95776508905434	39.0	36.0	40.0	31.0	40.0
21	36.76417257029029	39.0	36.0	40.0	31.0	40.0
22	36.731670112281286	39.0	36.0	40.0	31.0	40.0
23	36.3825293360638	39.0	36.0	40.0	30.0	40.0
24	36.49167287590444	39.0	36.0	40.0	30.0	40.0
25	36.46835775553878	39.0	36.0	40.0	30.0	40.0
26	36.395909087826254	38.0	35.0	40.0	30.0	40.0
27	36.33569744788886	38.0	35.0	40.0	30.0	40.0
28	36.229722425153206	38.0	35.0	40.0	30.0	40.0
29	36.100787274188086	38.0	35.0	40.0	30.0	40.0
30	36.04083721901212	38.0	35.0	40.0	30.0	40.0
31	35.8673461437449	38.0	35.0	40.0	29.0	40.0
32	35.71992509985739	38.0	35.0	40.0	28.0	40.0
33	35.57630833873661	38.0	34.0	40.0	27.0	40.0
34	35.4606120013699	38.0	34.0	40.0	27.0	40.0
35	35.35089478365027	38.0	34.0	40.0	27.0	40.0
36	35.23590473616745	38.0	34.0	40.0	27.0	40.0
37	35.0477949104891	38.0	33.0	40.0	27.0	40.0
38	35.23381867489122	38.0	34.0	40.0	27.0	40.0
39	35.2413416078078	38.0	34.0	40.0	27.0	40.0
40	35.09108713336572	38.0	33.0	40.0	26.0	40.0
41	35.38487698210146	38.0	34.0	40.0	27.0	40.0
42	35.366229914588494	38.0	34.0	40.0	27.0	40.0
43	35.45589459810374	38.0	34.0	40.0	27.0	40.0
44	35.450729567288406	38.0	34.0	40.0	27.0	40.0
45	35.377379854764214	38.0	34.0	40.0	27.0	40.0
46	35.20874304055783	38.0	34.0	40.0	27.0	40.0
47	35.1665543370384	38.0	34.0	40.0	27.0	40.0
48	35.102446606023186	38.0	34.0	40.0	26.0	40.0
49	34.95828313054541	38.0	33.0	40.0	26.0	40.0
50	34.832216920376496	38.0	33.0	40.0	26.0	40.0
51	34.635837123344	37.0	33.0	40.0	26.0	40.0
52	34.33391741431624	37.0	33.0	40.0	24.0	40.0
53	33.70696799716668	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	10.0
11	12.0
12	37.0
13	103.0
14	250.0
15	706.0
16	1656.0
17	3852.0
18	7842.0
19	15005.0
20	24851.0
21	38557.0
22	56112.0
23	78333.0
24	105212.0
25	137549.0
26	175008.0
27	219326.0
28	272029.0
29	336320.0
30	411161.0
31	505114.0
32	626764.0
33	790442.0
34	1027247.0
35	1381900.0
36	1932750.0
37	2813396.0
38	3889416.0
39	3284653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.860968746962124	46.87564186553826	28.926907516167223	15.336481871332388
2	29.635678705759766	44.60104546783172	14.891611328494934	10.87166449791358
3	36.1304191923372	25.687822076926764	21.62327791180811	16.55848081892793
4	21.966348752589727	26.858281548023772	20.661716811006055	30.51365288838045
5	18.59979036826602	35.58502819838513	24.997991520882145	20.817189912466702
6	28.433949268767478	24.998862734885222	23.285697594010195	23.281490402337106
7	21.93026505362681	24.97176136257429	31.40026201485442	21.697711568944484
8	26.07232520896867	26.30436589047197	26.239267456798952	21.384041443760406
9	28.0295019528703	25.062301450742247	25.851775729885723	21.05642086650173
10	21.231292264562548	26.429335473799537	31.697075803282743	20.642296458355172
11	20.607624346637746	33.31923216491221	25.484922952425155	20.58822053602489
12	20.698473219515655	25.929253122020192	32.34916845656113	21.023105201903018
13	20.649321310506572	26.3002028109003	25.29637128891094	27.75410458968219
14	20.93416969142427	33.307625168225634	24.921021417914023	20.83718372243607
15	21.006932602719303	32.993883360876744	24.832675906791792	21.16650812961216
16	21.10459128125418	25.95181646189737	24.97452388292582	27.969068373922624
17	28.18236141232171	25.67146200131201	25.069397985058462	21.076778601307826
18	21.485940398044445	25.300953433446118	24.94258120748386	28.27052496102558
19	21.18315493388616	32.99476560290518	24.716098650759697	21.105980812448966
20	28.49539742604785	25.96169205860315	24.815736859845874	20.727173655503126
21	21.70320352557148	32.48196793789104	24.66470253859078	21.15012599794669
22	28.1852838390409	25.928663122663682	24.943430365436228	20.942622672859194
23	21.507638037931223	25.68218124195747	31.984184929398307	20.825995790713
24	21.587083932591636	32.20817515239215	24.809721072014497	21.395019843001723
25	21.140129092962006	25.961019349056468	24.74798618607488	28.150865371906647
26	21.33524794557537	28.966812425915794	28.682460306139085	21.01547932236975
27	21.612067924034328	25.245300503490007	31.830735470590376	21.31189610188528
28	24.616488011736905	25.63387297688807	28.64252782632713	21.107111185047895
29	24.89034145137526	25.61170113191101	28.4551175634372	21.042839853276536
30	25.04637146811635	25.24804096779083	28.423020495640262	21.28256706845255
31	23.76683931224161	26.0980425640975	28.646905952393226	21.48821217126766
32	23.064751106014448	26.427493793565183	28.92320761366048	21.58454748675989
33	23.09493481141222	25.897972128099557	28.88400849753466	22.123084562953565
34	22.44773308737896	26.3096758846806	29.048640374052976	22.193950653887466
35	22.340518624873614	26.268828078764145	29.417031561050628	21.97362173531162
36	22.65611865449489	25.980776056480693	29.26681882768451	22.096286461339908
37	22.686401612120857	26.312554199298365	29.115977496873143	21.885066691707635
38	22.622836073972245	26.27270442967657	29.395543453645594	21.708916042705585
39	22.791145796946594	25.987045488895248	29.28478899500116	21.937019719156996
40	22.571224915308903	26.535838628669456	29.038643469068294	21.85429298695335
41	22.52388711647078	26.571641112985816	29.12961365022511	21.774858120318292
42	22.835401262697875	26.125259730674667	29.14666849143726	21.892670515190197
43	22.3879391339019	26.675701560239514	29.29337982675303	21.64297947910556
44	22.603443291384746	26.355756488628202	29.262330421364858	21.77846979862219
45	22.839420977939927	25.878612429588127	29.1418106462682	22.14015594620375
46	22.440096179820333	26.249942585342996	29.06191811658089	22.248043118255776
47	22.427419464674283	26.304834581549574	29.181754154105516	22.085991799670627
48	22.496317053082244	25.8929157784741	29.157900535261756	22.452866633181905
49	22.228060336311763	26.5843343701699	28.961893926607278	22.22571136691106
50	22.683082176488877	26.465562537092076	28.939043858070857	21.91231142834819
51	22.7636143316468	26.064434656826872	28.945682729334816	22.22626828219151
52	22.751351167451578	26.457699555013665	28.67508807118899	22.11586120634577
53	22.98199680374741	26.510115759527952	28.354238701498534	22.1536487352261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32381.0
1	18786.5
2	5192.0
3	4040.0
4	2888.0
5	2830.0
6	2772.0
7	3049.0
8	3988.5
9	4651.0
10	6150.5
11	7650.0
12	10327.5
13	13005.0
14	16773.0
15	20541.0
16	28333.0
17	36125.0
18	48762.0
19	61399.0
20	82727.0
21	104055.0
22	383459.0
23	662863.0
24	483482.0
25	312397.0
26	320693.0
27	353385.0
28	386077.0
29	419370.0
30	452663.0
31	479467.5
32	506272.0
33	551241.0
34	596210.0
35	654515.5
36	712821.0
37	769682.5
38	826544.0
39	877719.0
40	928894.0
41	975868.5
42	1059293.0
43	1095743.0
44	1131854.5
45	1167966.0
46	1182535.5
47	1197105.0
48	1200031.0
49	1202957.0
50	1191578.5
51	1180200.0
52	1151663.5
53	1123127.0
54	1069875.5
55	1016624.0
56	962789.5
57	908955.0
58	811780.5
59	618848.5
60	523091.0
61	450818.0
62	378545.0
63	318312.0
64	258079.0
65	208930.5
66	159782.0
67	126156.5
68	92531.0
69	71367.5
70	50204.0
71	37938.0
72	25672.0
73	19462.5
74	13253.0
75	9862.0
76	4833.5
77	3196.0
78	2538.0
79	1880.0
80	1446.5
81	1013.0
82	702.5
83	392.0
84	278.0
85	164.0
86	105.0
87	46.0
88	33.0
89	20.0
90	17.5
91	15.0
92	10.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8135613E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.68180622610664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.78663911811714	31.181930862469432
2	15.560243554163058	13.905195746362775
3	5.7449823951871215	7.7008857046243495
4	2.6344116167362572	4.70841077515255
5	1.4564119299843228	3.2537557820474747
6	0.9891947306323475	2.6519404364400185
7	0.6709887996810268	2.0986694069064864
8	0.473774295747976	1.6935273022016966
9	0.3629225909927747	1.459443319723414
>10	2.0912715566726225	17.082681122550333
>50	0.15699233435617352	4.808665989046363
>100	0.07003078935598357	5.191708003916857
>500	0.0013936163156399624	0.4099160937017542
>1k	5.694415988879735E-4	0.4370549229380468
>5k	8.66152293612826E-5	0.2609956941386435
>10k+	8.661522936128208E-5	3.155218837779656
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	453422	2.5001746563515663	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28127	0.1550926345858836	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	24269	0.1338195736752874	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	21022	0.1159155745107706	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1579426623186103E-4	0.0	0.0	0.0	0.0
2	0.0011414006242854873	0.0	0.0	0.0	5.514012677707668E-6
3	0.0011579426623186102	0.0	0.0	0.0	1.1028025355415337E-5
4	0.0013674751440715018	0.0	0.0	5.514012677707668E-6	1.1028025355415337E-5
5	0.0013729891567492093	0.0	0.0	5.514012677707668E-6	1.1028025355415337E-5
6	0.0013729891567492093	0.0	0.0	5.514012677707668E-6	2.757006338853834E-5
7	0.0013729891567492093	0.0	0.0	5.514012677707668E-6	2.757006338853834E-5
8	0.0013729891567492093	0.0	0.0	5.514012677707668E-6	2.757006338853834E-5
9	0.0013895311947823325	0.0	0.0	5.514012677707668E-6	3.308407606624601E-5
10	0.0014005592201377477	0.0	0.0	5.514012677707668E-6	3.859808874395368E-5
11	0.0014005592201377477	0.0	0.0	1.1028025355415337E-5	6.065413945478435E-5
12	0.0014060732328154554	0.0	0.0	1.1028025355415337E-5	6.616815213249202E-5
13	0.0016376617652791776	0.0	0.0	1.1028025355415337E-5	6.616815213249202E-5
14	0.001665231828667716	0.0	0.0	1.6542038033123006E-5	6.616815213249202E-5
15	0.001974016538619345	0.0	0.0	1.6542038033123006E-5	7.168216481019969E-5
16	0.002023642652718714	0.0	0.0	1.6542038033123006E-5	7.168216481019969E-5
17	0.002029156665396422	0.0	0.0	1.6542038033123006E-5	8.822420284332269E-5
18	0.002161492969661406	0.0	0.0	1.6542038033123006E-5	8.822420284332269E-5
19	0.002194577045727652	0.0	0.0	1.6542038033123006E-5	9.373821552103036E-5
20	0.002211119083760775	0.0	0.0	1.6542038033123006E-5	9.373821552103036E-5
21	0.0022662592105378515	0.0	0.0	1.6542038033123006E-5	9.925222819873803E-5
22	0.0022883152612486824	0.0	0.0	1.6542038033123006E-5	1.047662408764457E-4
23	0.0024041095274805434	0.0	0.0	1.6542038033123006E-5	1.2682229158727636E-4
24	0.002431679590869082	0.0	0.0	1.6542038033123006E-5	1.2682229158727636E-4
25	0.0024537356415799123	0.0	0.0	1.6542038033123006E-5	1.2682229158727636E-4
26	0.0024978477430015735	0.0	0.0	1.6542038033123006E-5	1.378503169426917E-4
27	0.002641212072621973	0.0	0.0	1.6542038033123006E-5	1.378503169426917E-4
28	0.0026742961486882193	0.0	0.0	1.6542038033123006E-5	1.4336432962039938E-4
29	0.0029775668459621407	1.1028025355415337E-5	0.0	1.6542038033123006E-5	1.4887834229810703E-4
30	0.0030657910488054636	1.1028025355415337E-5	0.0	1.6542038033123006E-5	1.4887834229810703E-4
31	0.003291865568591478	1.1028025355415337E-5	0.0	1.6542038033123006E-5	1.4887834229810703E-4
32	0.0033139216193023087	1.1028025355415337E-5	0.0	1.6542038033123006E-5	1.5439235497581472E-4
33	0.0033635477334016776	7.719617748790736E-5	0.0	1.6542038033123006E-5	1.5439235497581472E-4
34	0.003402145822145631	7.719617748790736E-5	0.0	1.6542038033123006E-5	1.5990636765352237E-4
35	0.0034793419996335386	7.719617748790736E-5	0.0	1.6542038033123006E-5	1.7093439300893773E-4
36	0.0036171923165762305	7.719617748790736E-5	0.0	1.6542038033123006E-5	1.929904437197684E-4
37	0.0038267247983291217	7.719617748790736E-5	0.0	1.6542038033123006E-5	2.0401846907518374E-4
38	0.003986631165982644	7.719617748790736E-5	0.0	1.6542038033123006E-5	2.0401846907518374E-4
39	0.004036257280082013	7.719617748790736E-5	0.0	1.6542038033123006E-5	2.0401846907518374E-4
40	0.0041189674702476285	7.719617748790736E-5	0.0	1.6542038033123006E-5	2.0401846907518374E-4
41	0.00418513562238012	7.719617748790736E-5	0.0	1.6542038033123006E-5	2.0401846907518374E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	135685	0.0	44.476543	12
CAACGCA	135775	0.0	44.45226	13
AACGCAG	136580	0.0	44.148956	14
ATCAACG	137380	0.0	44.12793	11
ACGCAGA	136490	0.0	44.09887	15
GGTATCA	137010	0.0	44.032696	8
GTATCAA	137005	0.0	44.00343	9
GTGGTAT	136810	0.0	43.933884	6
TGGTATC	137225	0.0	43.857533	7
AGTGGTA	136920	0.0	43.84195	5
CGCAGAG	137695	0.0	43.71295	16
AAGCAGT	139245	0.0	43.69047	1
AGAGTAC	135430	0.0	43.441074	19
TATCAAC	138960	0.0	43.386047	10
CAGTGGT	140660	0.0	42.861687	4
GAGTACT	73010	0.0	42.73839	20
CAGAGTA	137595	0.0	42.661907	18
GCAGTGG	142425	0.0	42.634125	3
GCAGAGT	138530	0.0	42.499493	17
AGCAGTG	143215	0.0	42.46294	2
>>END_MODULE