FastQCFastQC Report
Thu 26 May 2016
SRR805322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22751822
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4649152.043418764440052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG358800.15770165571794645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA286720.1260206764979086No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG244770.10758259272598035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1885700.044.77424612
CAACGCA1889200.044.711213
AACGCAG1894150.044.55217714
ATCAACG1906950.044.48357411
ACGCAGA1896400.044.46709415
GTATCAA1900100.044.4225549
GTGGTAT1901100.044.4028976
AGTGGTA1897450.044.3892335
GGTATCA1906550.044.3819738
TGGTATC1904300.044.3739437
AAGCAGT1927700.044.2765961
CGCAGAG1918050.044.03133416
AGAGTAC1876950.043.98636219
TATCAAC1921100.043.93696210
CAGTGGT1955550.043.291534
CAGAGTA1902000.043.28225718
GCAGTGG1978300.043.083533
AGCAGTG1982250.043.0474742
GCAGAGT1928450.042.9250217
GAGTACT794600.042.83885220
Produced by FastQC (version 0.11.5)