##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805322_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22751822
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.99760889479533	35.0	35.0	37.0	33.0	37.0
2	35.3823530704486	35.0	35.0	37.0	33.0	37.0
3	37.25062581801141	39.0	37.0	39.0	34.0	39.0
4	37.28938073618895	39.0	37.0	39.0	34.0	39.0
5	37.26933768205465	39.0	37.0	39.0	34.0	39.0
6	37.176398575903065	39.0	37.0	39.0	34.0	39.0
7	37.0407129152118	39.0	37.0	39.0	33.0	39.0
8	37.96727382976185	40.0	38.0	40.0	33.0	40.0
9	37.96588919340174	40.0	38.0	40.0	33.0	40.0
10	37.669668609397526	40.0	37.0	40.0	33.0	40.0
11	37.783746813771664	40.0	37.0	40.0	33.0	40.0
12	37.74831237691645	40.0	37.0	40.0	33.0	40.0
13	37.86404666843825	40.0	38.0	40.0	33.0	40.0
14	37.79770020176846	40.0	37.0	40.0	33.0	40.0
15	37.71260881875746	40.0	37.0	40.0	33.0	40.0
16	37.630099602572486	40.0	37.0	40.0	33.0	40.0
17	37.51240929188001	40.0	37.0	40.0	32.0	40.0
18	37.301666829144494	39.0	37.0	40.0	32.0	40.0
19	37.3396966185829	39.0	37.0	40.0	32.0	40.0
20	37.223678437709296	39.0	37.0	40.0	32.0	40.0
21	37.047391281454296	39.0	36.0	40.0	32.0	40.0
22	37.03732461514511	39.0	36.0	40.0	31.0	40.0
23	36.65835184540386	39.0	36.0	40.0	30.0	40.0
24	36.7907015974369	39.0	36.0	40.0	31.0	40.0
25	36.79295152713484	39.0	36.0	40.0	31.0	40.0
26	36.714240028776594	39.0	36.0	40.0	30.0	40.0
27	36.64864664465114	39.0	36.0	40.0	30.0	40.0
28	36.551126059266814	39.0	36.0	40.0	30.0	40.0
29	36.42843746755754	39.0	36.0	40.0	30.0	40.0
30	36.370334560458495	39.0	35.0	40.0	30.0	40.0
31	36.18426352843302	38.0	35.0	40.0	30.0	40.0
32	36.00048154385174	38.0	35.0	40.0	29.0	40.0
33	35.86090507388815	38.0	35.0	40.0	29.0	40.0
34	35.76539426161123	38.0	35.0	40.0	29.0	40.0
35	35.68622943692158	38.0	34.0	40.0	28.0	40.0
36	35.56222490664704	38.0	34.0	40.0	28.0	40.0
37	35.45056892586449	38.0	34.0	40.0	27.0	40.0
38	35.58819192590378	38.0	34.0	40.0	28.0	40.0
39	35.593618436360835	38.0	34.0	40.0	28.0	40.0
40	35.375706569785926	38.0	34.0	40.0	27.0	40.0
41	35.666880524997076	38.0	34.0	40.0	28.0	40.0
42	35.512598815163024	38.0	34.0	40.0	27.0	40.0
43	35.662155189153644	38.0	34.0	40.0	28.0	40.0
44	35.69735729296757	38.0	34.0	40.0	28.0	40.0
45	35.62276858530275	38.0	34.0	40.0	28.0	40.0
46	35.45579461723989	38.0	34.0	40.0	27.0	40.0
47	35.4120455495828	38.0	34.0	40.0	27.0	40.0
48	35.35039699238153	38.0	34.0	40.0	27.0	40.0
49	35.2157359529272	38.0	34.0	40.0	27.0	40.0
50	35.08313501222012	38.0	34.0	40.0	26.0	40.0
51	34.888117312099226	38.0	33.0	40.0	26.0	40.0
52	34.61029683688629	37.0	33.0	40.0	25.0	40.0
53	34.00013629677658	37.0	32.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	9.0
12	22.0
13	74.0
14	192.0
15	484.0
16	1257.0
17	3073.0
18	6894.0
19	13321.0
20	23535.0
21	37851.0
22	56511.0
23	81004.0
24	109934.0
25	146131.0
26	188042.0
27	239881.0
28	301754.0
29	376305.0
30	466192.0
31	578656.0
32	726364.0
33	923511.0
34	1206267.0
35	1630622.0
36	2298723.0
37	3392466.0
38	4978536.0
39	4964208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.726797089041924	37.72071968565858	28.152549716677637	22.399933508621857
2	27.649284527630357	42.53567033005093	14.129066234783307	15.685978907535405
3	33.304409642445336	26.6529203683116	19.761573380804403	20.281096608438656
4	21.527286913549165	24.957249577638223	20.940951454349456	32.574512054463156
5	20.166446449871138	33.479134110665946	24.312580328731475	22.04183911073144
6	29.76987513351678	24.334262108766495	22.874480118559294	23.021382639157427
7	22.511339970926283	23.957360425903474	31.439024971274826	22.092274631895414
8	28.77043429752571	25.544389368025115	24.25579806311776	21.429378271331412
9	29.42570049994238	24.768407558744087	24.290577695272052	21.51531424604148
10	21.413344390616277	25.334889662902604	31.911804689751882	21.339961256729243
11	21.229899741655856	33.22883327761618	24.248462386880487	21.29280459384747
12	21.004704590252157	25.041651609264527	32.358938110539015	21.594705689944306
13	21.10528994117482	25.16733385132848	24.44742227677414	29.279953930722556
14	21.46566987030753	33.27559876303533	23.9970495549763	21.26168181168084
15	21.34609702906431	33.026625296207044	24.064978180648566	21.56229949408008
16	21.457815554288356	24.830556427524794	24.261942625957605	29.449685392229245
17	29.53297102974874	24.762509130038023	24.16852593168143	21.535993908531808
18	21.688087222201368	24.679882780376886	24.199723433138672	29.432306564283074
19	21.539945240429535	32.78468862845358	24.10383221176748	21.571533919349402
20	29.74475187086116	25.001922922920194	24.04886518539043	21.204460020828222
21	21.787463878717052	32.60962133054663	24.09201337809341	21.51090141264291
22	29.432798832550645	24.70751573214664	24.35309137000105	21.50659406530167
23	21.837112649703393	24.792124340635226	32.016002938138314	21.354760071523064
24	21.714581803602368	32.38430750732842	24.166385443767975	21.734725245301235
25	21.452589599197815	24.841786297378732	24.174389198368377	29.531234905055076
26	21.7336571989707	29.388195811306893	27.395036757935255	21.48311023178715
27	21.84044864626666	24.640118932013443	31.99454531597513	21.52488710574476
28	26.273438672296223	24.623179629306172	27.50571800359549	21.59766369480211
29	26.581607398299795	24.781729568735198	27.175885078566452	21.46077795439855
30	26.503077423865218	24.5782249878713	27.262032025391196	21.65666556287228
31	24.902300132270724	25.30756437880008	27.66572716681767	22.12440832211152
32	24.00366880507416	25.926015947206338	27.770444054986015	22.299871192733487
33	23.664254229837063	25.480609860608087	28.13021304403665	22.7249228655182
34	23.060535547438793	25.523155903733773	28.35375118528969	23.062557363537742
35	23.0452444643774	25.686571387557443	28.51143525999808	22.756748888067076
36	23.148792215410268	25.58054910942957	28.522946426004914	22.747712249155256
37	23.39547575574387	25.486798375971826	28.399242047516022	22.718483820768288
38	23.458345445916375	25.703739243388945	28.386807878507486	22.45110743218719
39	23.45005599991069	25.48701813859127	28.449202881421982	22.613722980076055
40	23.340759258752993	25.75043880002226	28.290604594216674	22.618197347008078
41	23.34474135741744	26.06150839260258	28.135808200327865	22.45794204965211
42	23.507638201459205	25.828775383351715	28.302581656976745	22.36100475821233
43	23.07800667568514	25.829408299695732	28.632643135130014	22.459941889489116
44	23.435753848636825	25.710516722572812	28.260835549785856	22.592893879004503
45	23.39560761331554	25.405974079790184	28.35056462730765	22.847853679586628
46	23.137663436361272	25.4844469159437	28.22205623795756	23.15583340973747
47	23.248177662430727	25.64691302525134	28.214549146877115	22.890360165440814
48	23.106123105217684	25.46816250584239	28.281181173094623	23.144533215845307
49	22.872831898913415	25.94140372582029	28.158153663473634	23.027610711792665
50	23.470740057653405	25.90546814228768	27.91717955599336	22.706612244065553
51	23.30447645028165	25.65818684762917	28.1514025558041	22.885934146285074
52	23.448368222993306	25.697796862158995	27.928637978971533	22.92519693587617
53	23.841268624552356	25.871633489397023	27.391590880062267	22.895507005988357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30423.0
1	16907.0
2	3391.0
3	2504.5
4	1618.0
5	1547.5
6	1477.0
7	1606.0
8	2085.5
9	2436.0
10	3501.5
11	4567.0
12	6580.0
13	8593.0
14	10916.5
15	13240.0
16	19350.5
17	25461.0
18	35658.0
19	45855.0
20	65135.0
21	84415.0
22	367485.0
23	650555.0
24	471614.0
25	299714.0
26	306755.0
27	340669.5
28	374584.0
29	413404.5
30	452225.0
31	487255.5
32	522286.0
33	579829.0
34	637372.0
35	713866.0
36	790360.0
37	868788.5
38	947217.0
39	1020842.5
40	1094468.0
41	1158709.5
42	1287381.0
43	1351811.0
44	1408718.5
45	1465626.0
46	1500010.0
47	1534394.0
48	1563260.0
49	1592126.0
50	1591121.0
51	1590116.0
52	1569091.5
53	1548067.0
54	1496821.0
55	1445575.0
56	1378484.5
57	1311394.0
58	1183221.5
59	922709.5
60	790370.0
61	683079.0
62	575788.0
63	489991.5
64	404195.0
65	328185.5
66	252176.0
67	200302.0
68	148428.0
69	115260.0
70	82092.0
71	62551.5
72	43011.0
73	32614.0
74	22217.0
75	17062.0
76	9091.0
77	6275.0
78	4783.5
79	3292.0
80	2521.0
81	1750.0
82	1343.5
83	937.0
84	640.5
85	344.0
86	226.0
87	108.0
88	87.0
89	66.0
90	47.5
91	29.0
92	21.0
93	9.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.2751822E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.15456117196218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.99604352280258	28.395018937825434
2	15.37806932388184	12.657553893927403
3	5.897051549683441	7.280717062069845
4	2.7329240597444167	4.498891615803153
5	1.575713047583361	3.2423889503114194
6	0.9751504452046384	2.4079133189424264
7	0.6934137686767031	1.997599755234035
8	0.5070149014637737	1.6692780621909806
9	0.3944369914172332	1.460959316258872
>10	2.545073762443756	19.867262578813175
>50	0.20717262351936838	5.83003378701866
>100	0.09508987360867248	6.651535276847312
>500	0.0019879416446090437	0.5291202609448171
>1k	7.082125618339865E-4	0.5675412004628602
>5k	6.427532736980401E-5	0.184905488861761
>10k+	8.57004364929819E-5	2.759280494487628
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	464915	2.043418764440052	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	35880	0.15770165571794645	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28672	0.1260206764979086	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	24477	0.10758259272598035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0218160989480315E-4	4.39525238901746E-6	0.0	0.0	1.3185757167052379E-5
2	0.0069269177650915165	4.39525238901746E-6	0.0	4.39525238901746E-6	1.3185757167052379E-5
3	0.006962079784203656	8.79050477803492E-6	0.0	8.79050477803492E-6	1.758100955606984E-5
4	0.00823670297701872	1.758100955606984E-5	0.0	8.79050477803492E-6	1.758100955606984E-5
5	0.008258679238963807	2.6371514334104758E-5	0.0	8.79050477803492E-6	3.9557271501157135E-5
6	0.008258679238963807	2.6371514334104758E-5	0.0	1.3185757167052379E-5	3.9557271501157135E-5
7	0.008258679238963807	3.076676672312222E-5	0.0	1.3185757167052379E-5	3.9557271501157135E-5
8	0.008258679238963807	3.076676672312222E-5	0.0	1.3185757167052379E-5	3.9557271501157135E-5
9	0.008258679238963807	3.516201911213968E-5	0.0	2.19762619450873E-5	4.834777627919206E-5
10	0.008271864996130859	3.516201911213968E-5	0.0	2.19762619450873E-5	5.713828105722698E-5
11	0.008280655500908894	3.516201911213968E-5	0.0	2.19762619450873E-5	7.911454300231427E-5
12	0.008359770043911209	3.516201911213968E-5	0.0	2.6371514334104758E-5	8.350979539133174E-5
13	0.00849602286797075	3.516201911213968E-5	0.0	2.6371514334104758E-5	8.79050477803492E-5
14	0.008517999129915837	3.516201911213968E-5	0.0	2.6371514334104758E-5	1.0548605733641903E-4
15	0.008728971244588676	3.516201911213968E-5	0.0	3.516201911213968E-5	1.098813097254365E-4
16	0.008759738011311797	3.516201911213968E-5	0.0	3.516201911213968E-5	1.2306706689248887E-4
17	0.00877292376847885	3.516201911213968E-5	0.0	3.516201911213968E-5	1.4504332883757617E-4
18	0.008922362349705443	3.516201911213968E-5	0.0	3.9557271501157135E-5	1.4943858122659363E-4
19	0.008948733864039549	3.516201911213968E-5	0.0	3.9557271501157135E-5	1.6701959078266348E-4
20	0.0089619196212066	3.516201911213968E-5	0.0	3.9557271501157135E-5	1.7141484317168094E-4
21	0.008979500630762671	3.9557271501157135E-5	0.0	4.39525238901746E-5	1.846006003387333E-4
22	0.008988291135540706	3.9557271501157135E-5	0.0	4.39525238901746E-5	1.9339110511676824E-4
23	0.009023453154652845	3.9557271501157135E-5	0.0	4.39525238901746E-5	2.065768622838206E-4
24	0.009093777192877125	3.9557271501157135E-5	0.0	4.834777627919206E-5	2.1536736706185552E-4
25	0.009137729716767299	3.9557271501157135E-5	0.0	5.2743028668209516E-5	2.1536736706185552E-4
26	0.00920365850260256	4.39525238901746E-5	0.0	5.2743028668209516E-5	2.19762619450873E-4
27	0.009317935064717014	4.39525238901746E-5	0.0	5.2743028668209516E-5	2.3294837661792537E-4
28	0.00934430657905112	4.39525238901746E-5	0.0	5.713828105722698E-5	2.505293861739952E-4
29	0.00948055940311066	4.39525238901746E-5	0.0	5.713828105722698E-5	2.505293861739952E-4
30	0.009559673946112976	4.39525238901746E-5	0.0	6.153353344624443E-5	2.5931989095203013E-4
31	0.009682741013005465	5.2743028668209516E-5	0.0	7.911454300231427E-5	2.5931989095203013E-4
32	0.009704717274950552	5.2743028668209516E-5	0.0	7.911454300231427E-5	2.5931989095203013E-4
33	0.009783831817952865	5.2743028668209516E-5	0.0	7.911454300231427E-5	2.8569140528613487E-4
34	0.009876132118122232	5.713828105722698E-5	0.0	7.911454300231427E-5	2.8569140528613487E-4
35	0.009937665651568476	6.153353344624443E-5	0.0	7.911454300231427E-5	2.9887716245318726E-4
36	0.010038756456515878	6.153353344624443E-5	0.0	8.350979539133174E-5	3.2085342439827457E-4
37	0.010179404532964436	6.153353344624443E-5	0.0	8.350979539133174E-5	3.2085342439827457E-4
38	0.010254123823577734	6.153353344624443E-5	0.0	8.79050477803492E-5	3.2085342439827457E-4
39	0.01031126210463496	6.153353344624443E-5	0.0	8.79050477803492E-5	3.2085342439827457E-4
40	0.010438724423916468	6.153353344624443E-5	0.0	8.79050477803492E-5	3.296439291763095E-4
41	0.010526629471696816	6.153353344624443E-5	0.0	8.79050477803492E-5	3.4282968634336187E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	188570	0.0	44.774246	12
CAACGCA	188920	0.0	44.7112	13
AACGCAG	189415	0.0	44.552177	14
ATCAACG	190695	0.0	44.483574	11
ACGCAGA	189640	0.0	44.467094	15
GTATCAA	190010	0.0	44.422554	9
GTGGTAT	190110	0.0	44.402897	6
AGTGGTA	189745	0.0	44.389233	5
GGTATCA	190655	0.0	44.381973	8
TGGTATC	190430	0.0	44.373943	7
AAGCAGT	192770	0.0	44.276596	1
CGCAGAG	191805	0.0	44.031334	16
AGAGTAC	187695	0.0	43.986362	19
TATCAAC	192110	0.0	43.936962	10
CAGTGGT	195555	0.0	43.29153	4
CAGAGTA	190200	0.0	43.282257	18
GCAGTGG	197830	0.0	43.08353	3
AGCAGTG	198225	0.0	43.047474	2
GCAGAGT	192845	0.0	42.92502	17
GAGTACT	79460	0.0	42.838852	20
>>END_MODULE