FastQCFastQC Report
Thu 26 May 2016
SRR805321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20594628
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2513891.2206532693865604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524490.25467320895526735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC864250.042.97026412
AAGCAGT872650.042.8744051
CAACGCA866550.042.86705813
ACGCAGA869850.042.63689415
AACGCAG871300.042.6333614
GGTATCA881250.042.2613338
GTATCAA880500.042.177239
ATCAACG885300.042.14232311
GTGGTAT882900.042.030646
TGGTATC885000.042.0264977
CGCAGAG886650.041.8846816
AGTGGTA884800.041.884615
TATCAAC889100.041.7692610
AGAGTAC875050.041.60202419
CAGAGTA898700.040.40263718
GCAGTGG930100.040.2590073
GAGTACT436550.040.2280420
GCAGAGT907000.040.19355417
AGCAGTG931950.040.1740462
CAGTGGT930900.040.0401234