##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20594628
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.80371327901626	35.0	35.0	37.0	32.0	37.0
2	35.252021061026205	35.0	35.0	37.0	33.0	37.0
3	37.15704537124924	39.0	37.0	39.0	33.0	39.0
4	37.289576048666675	39.0	37.0	39.0	34.0	39.0
5	37.28719363127122	39.0	37.0	39.0	34.0	39.0
6	37.13365232914137	39.0	37.0	39.0	33.0	39.0
7	37.081907961629604	39.0	37.0	39.0	33.0	39.0
8	37.95996587071153	40.0	38.0	40.0	33.0	40.0
9	37.93197143449253	40.0	38.0	40.0	33.0	40.0
10	37.79575940871571	40.0	37.0	40.0	33.0	40.0
11	37.837214491079905	40.0	37.0	40.0	33.0	40.0
12	37.72973388982797	40.0	37.0	40.0	33.0	40.0
13	37.840011531162396	40.0	37.0	40.0	33.0	40.0
14	37.74771751157632	40.0	37.0	40.0	33.0	40.0
15	37.65987052545936	40.0	37.0	40.0	33.0	40.0
16	37.59307524272835	40.0	37.0	40.0	33.0	40.0
17	37.47722478891097	40.0	37.0	40.0	32.0	40.0
18	37.290438603698014	40.0	37.0	40.0	32.0	40.0
19	37.317426660971975	39.0	37.0	40.0	32.0	40.0
20	37.15281043192429	39.0	37.0	40.0	32.0	40.0
21	37.06310048426221	39.0	36.0	40.0	32.0	40.0
22	36.97083142264089	39.0	36.0	40.0	31.0	40.0
23	36.80277890914077	39.0	36.0	40.0	31.0	40.0
24	36.82631072530177	39.0	36.0	40.0	31.0	40.0
25	36.79057344468664	39.0	36.0	40.0	31.0	40.0
26	36.68446290945386	39.0	36.0	40.0	30.0	40.0
27	36.57411617243098	39.0	36.0	40.0	30.0	40.0
28	36.46768938967968	39.0	36.0	40.0	30.0	40.0
29	36.342542579550354	39.0	35.0	40.0	30.0	40.0
30	36.265960278573615	38.0	35.0	40.0	30.0	40.0
31	36.11567662207834	38.0	35.0	40.0	30.0	40.0
32	35.957631087097084	38.0	35.0	40.0	29.0	40.0
33	35.850130529184604	38.0	35.0	40.0	29.0	40.0
34	35.75105221614103	38.0	35.0	40.0	28.0	40.0
35	35.67222020227799	38.0	34.0	40.0	28.0	40.0
36	35.56644460875914	38.0	34.0	40.0	27.0	40.0
37	35.46974754775857	38.0	34.0	40.0	27.0	40.0
38	35.64004006287465	38.0	34.0	40.0	28.0	40.0
39	35.63705792597953	38.0	34.0	40.0	28.0	40.0
40	35.46811299529178	38.0	34.0	40.0	27.0	40.0
41	35.673887821620276	38.0	34.0	40.0	28.0	40.0
42	35.50706276413442	38.0	34.0	40.0	27.0	40.0
43	35.64793498576425	38.0	34.0	40.0	27.0	40.0
44	35.67509988527105	38.0	34.0	40.0	28.0	40.0
45	35.59718325575	38.0	34.0	40.0	27.0	40.0
46	35.44014900390529	38.0	34.0	40.0	27.0	40.0
47	35.41731669054668	38.0	34.0	40.0	27.0	40.0
48	35.36402939640376	38.0	34.0	40.0	27.0	40.0
49	35.252584751712924	38.0	34.0	40.0	27.0	40.0
50	35.12928852125904	38.0	34.0	40.0	26.0	40.0
51	34.97270326999837	38.0	33.0	40.0	26.0	40.0
52	34.73980947847176	38.0	33.0	40.0	26.0	40.0
53	34.16666117979893	37.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	10.0
12	28.0
13	62.0
14	209.0
15	568.0
16	1379.0
17	3312.0
18	7241.0
19	13856.0
20	24357.0
21	38808.0
22	58471.0
23	81364.0
24	109742.0
25	143630.0
26	184042.0
27	231137.0
28	287061.0
29	353123.0
30	433288.0
31	531150.0
32	656009.0
33	821740.0
34	1055966.0
35	1406761.0
36	1947840.0
37	2871446.0
38	4442198.0
39	4889827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.297079218910874	32.16731081522813	16.575599229080513	37.96001073678049
2	21.84271063308354	27.650550425091435	29.275707237829206	21.231031703995818
3	18.93811337597358	24.36886939642707	31.341901392926346	25.351115834673006
4	16.74875603482617	27.06361095718748	30.362650881579412	25.824982126406944
5	21.582900162119948	30.56766065403075	28.50924037083845	19.34019881301085
6	24.26174922897369	25.87201380864952	29.22851531962607	20.63772164275072
7	22.13708351517687	27.257986888619694	30.528767016330665	20.07616257987277
8	23.284936246481365	25.29001737734714	29.206038584430853	22.21900779174064
9	23.54474186180979	24.77858303631413	30.25618136923862	21.420493732637464
10	21.197474409345972	26.016915673349377	31.021317792193187	21.76429212511146
11	21.008148338489047	30.050283015551432	27.196257198721916	21.7453114472376
12	21.052591967186782	26.28983150363289	30.810631782229812	21.846944746950516
13	21.431040172223554	26.652591151440074	26.25143314072	25.664935535616372
14	21.773119669847883	30.432785675953944	26.098869083724164	21.69522557047401
15	21.534450634408156	30.273501419884834	26.338528668738277	21.853519276968733
16	21.83684502579993	26.54547583962187	25.85507249754645	25.762606637031755
17	25.879010778927398	26.190757123653803	26.057241723424184	21.872990373994618
18	22.090066399839802	26.21184514719081	25.856602022624543	25.841486430344844
19	22.148324310592063	30.026237910196773	25.627212105991916	22.198225673219248
20	25.918351135062988	26.30700588522405	25.713336506976482	22.061306472736483
21	22.2530263717315	29.94639184548514	25.915573711746575	21.885008071036776
22	25.76698156431862	26.096237329462806	25.99782331586664	22.13895779035193
23	21.974657663153714	26.27626485897196	29.88612855740827	21.862948920466057
24	21.828978896826882	29.96083736011158	26.106332194978226	22.103851548083316
25	21.869334080712697	26.153130806732705	25.960823375882292	26.0167117366723
26	21.92202257792663	28.03936055557789	28.106941285853765	21.93167558064171
27	21.942120051889262	25.96796115958006	30.078071815621044	22.011846972909634
28	24.038166652002648	25.97938646913166	27.73212509592307	22.25032178294262
29	24.112375324283594	26.128527303333666	27.792883658787137	21.966213713595604
30	23.905088258938203	25.97018504048726	27.924777276870454	22.199949423704084
31	23.25696778791052	26.300722693315947	27.7896935064814	22.652616012292135
32	23.155514146698838	26.733626846768	27.91909132808808	22.19176767844508
33	22.76926293594621	26.718181071296843	27.929336718293722	22.58321927446322
34	22.78382498581669	26.631134099630255	27.828305517341708	22.75673539721135
35	22.731073365345562	26.823922238362353	27.916576109070775	22.52842828722131
36	22.706125111849556	26.707527807737048	27.952240749383773	22.634106331029628
37	22.86254939880439	26.604991359882778	27.735208424255102	22.79725081705773
38	22.944818425465126	26.75744373726974	28.007575567764565	22.29016226950057
39	22.871765394354295	26.64181164136589	27.95230387264096	22.53411909163885
40	22.98832491657533	26.683409867854863	27.745366413027707	22.5828988025421
41	22.76679141764542	26.91715528923368	28.023196146101792	22.292857147019117
42	22.889983737506693	26.629939613378788	28.152356041585215	22.327720607529304
43	22.622054644541283	26.647837484610065	28.038501108153056	22.691606762695592
44	22.80980263396843	26.644642476669155	28.026274618798652	22.519280270563762
45	22.772326841737563	26.455816536234593	28.114147048443893	22.657709573583944
46	22.676462036604885	26.499682344347274	27.899052121747474	22.924803497300363
47	22.755953639949215	26.60499621551795	27.960801234185922	22.678248910346912
48	22.680443657443096	26.537216404200166	28.0384331292607	22.743906809096043
49	22.760280010884394	26.74381396935162	27.66534554545001	22.830560474313984
50	22.898573356119858	26.80472305690591	27.87115649770416	22.425547089270076
51	22.782635355200394	26.62291836492507	27.93222582121901	22.66222045865553
52	22.960026274813025	26.66763390919224	27.44590482527774	22.926434990716995
53	22.975020476213505	26.835604896577888	27.457082497435735	22.732292129772873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55557.0
1	32950.5
2	10344.0
3	7853.0
4	5362.0
5	5111.5
6	4861.0
7	5354.5
8	6797.5
9	7747.0
10	9877.0
11	12007.0
12	15813.0
13	19619.0
14	24689.5
15	29760.0
16	39459.0
17	49158.0
18	65544.0
19	81930.0
20	109927.5
21	137925.0
22	308094.5
23	478264.0
24	395964.5
25	346289.0
26	378913.0
27	423147.5
28	467382.0
29	502755.5
30	538129.0
31	567572.0
32	597015.0
33	642410.5
34	687806.0
35	747957.5
36	808109.0
37	873512.0
38	938915.0
39	992875.0
40	1046835.0
41	1086173.5
42	1168384.0
43	1211256.0
44	1243967.0
45	1276678.0
46	1297045.0
47	1317412.0
48	1328208.0
49	1339004.0
50	1333842.5
51	1328681.0
52	1298880.0
53	1269079.0
54	1220078.5
55	1171078.0
56	1101483.0
57	1031888.0
58	935848.5
59	743324.5
60	646840.0
61	570679.0
62	494518.0
63	425867.5
64	357217.0
65	291748.5
66	226280.0
67	180701.0
68	135122.0
69	103746.5
70	72371.0
71	56018.5
72	39666.0
73	28582.5
74	17499.0
75	13264.5
76	6915.0
77	4800.0
78	3569.0
79	2338.0
80	2006.5
81	1675.0
82	1399.5
83	1124.0
84	743.5
85	363.0
86	246.0
87	129.0
88	94.5
89	60.0
90	45.0
91	30.0
92	20.0
93	7.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0594628E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.8961055934853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.06584412661335	20.686113050834138
2	19.40930507330733	14.322555389618344
3	9.493071783661627	10.507721368095456
4	5.093460330345865	7.517154007386782
5	2.8744199786710256	5.302745152653496
6	1.8027572221483643	3.990883249668256
7	1.1375911654820854	2.9380878634690117
8	0.7665214673716849	2.2625325599855186
9	0.5347679903583051	1.7757770616248356
>10	2.577268819369253	16.421385329452637
>50	0.1475589002528031	3.733855950813916
>100	0.08993378804312038	6.373317526658771
>500	0.0056026608676171055	1.368697664507656
>1k	0.001777498275681301	0.9710357728319722
>5k	5.297565862189247E-5	0.14946791745659754
>10k+	6.621957327736502E-5	1.678670134942362
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	251389	1.2206532693865604	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52449	0.25467320895526735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012139087921374447	0.0	0.0	0.0	3.884508134839823E-5
2	0.0015295250780931805	0.0	0.0	0.0	3.884508134839823E-5
3	0.0015538032539359292	0.0	0.0	0.0	3.884508134839823E-5
4	0.001582937064947228	0.0	0.0	0.0	4.370071651694801E-5
5	0.002112201298319154	0.0	0.0	0.0	4.370071651694801E-5
6	0.0021364794741619026	0.0	0.0	0.0	5.8267622022597346E-5
7	0.0021364794741619026	0.0	0.0	4.855635168549779E-6	6.312325719114714E-5
8	0.0021413351093304525	0.0	0.0	4.855635168549779E-6	6.312325719114714E-5
9	0.0021413351093304525	0.0	0.0	4.855635168549779E-6	6.312325719114714E-5
10	0.0021413351093304525	0.0	0.0	9.711270337099558E-6	6.797889235969691E-5
11	0.002175324555510301	0.0	0.0	9.711270337099558E-6	6.797889235969691E-5
12	0.0022044583665216	0.0	0.0	9.711270337099558E-6	6.797889235969691E-5
13	0.0023938281380950412	0.0	0.0	9.711270337099558E-6	6.797889235969691E-5
14	0.0024326732194434393	4.855635168549779E-6	0.0	1.4566905505649337E-5	7.283452752824669E-5
15	0.0026268986261854307	4.855635168549779E-6	0.0	1.4566905505649337E-5	7.769016269679646E-5
16	0.002660888072365279	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.2624651438229427E-4
17	0.002680310613039478	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.5052469022504316E-4
18	0.002791990221916123	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.5052469022504316E-4
19	0.0028065571274217722	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.650915957306925E-4
20	0.0028211240329274216	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.650915957306925E-4
21	0.0028599691142758197	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.6994723089924226E-4
22	0.002874536019781469	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.7480286606779205E-4
23	0.002961937452815365	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.845141364048916E-4
24	0.0029959268989952137	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.845141364048916E-4
25	0.0030202050748379624	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.893697715734414E-4
26	0.0030784726968605598	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.9422540674199115E-4
27	0.0031561628595573564	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.9422540674199115E-4
28	0.003233853022254153	4.855635168549779E-6	0.0	1.4566905505649337E-5	1.9422540674199115E-4
29	0.003471779145513092	4.855635168549779E-6	0.0	1.9422540674199115E-5	1.9908104191054094E-4
30	0.0035543249433784385	4.855635168549779E-6	0.0	1.9422540674199115E-5	2.0393667707909073E-4
31	0.0037436947149518795	9.711270337099558E-6	0.0	1.9422540674199115E-5	2.1364794741619028E-4
32	0.003865085594165624	9.711270337099558E-6	0.0	2.9133811011298673E-5	2.4278175842748896E-4
33	0.004025321554727767	9.711270337099558E-6	0.0	3.3989446179848455E-5	2.6220429910168806E-4
34	0.004214691326301208	9.711270337099558E-6	0.0	3.3989446179848455E-5	2.6220429910168806E-4
35	0.004491462530908545	9.711270337099558E-6	0.0	3.3989446179848455E-5	2.86482474944437E-4
36	0.004957603507089324	9.711270337099558E-6	0.0	3.3989446179848455E-5	3.787395431468828E-4
37	0.005146973278662766	9.711270337099558E-6	0.0	3.3989446179848455E-5	3.884508134839823E-4
38	0.005219807806191012	9.711270337099558E-6	0.0	3.3989446179848455E-5	3.981620838210819E-4
39	0.0052877866985507095	9.711270337099558E-6	0.0	3.3989446179848455E-5	3.981620838210819E-4
40	0.005316920509562008	9.711270337099558E-6	0.0	3.3989446179848455E-5	3.981620838210819E-4
41	0.005370332496416056	9.711270337099558E-6	0.0	3.3989446179848455E-5	4.0787335415818146E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	86425	0.0	42.970264	12
AAGCAGT	87265	0.0	42.874405	1
CAACGCA	86655	0.0	42.867058	13
ACGCAGA	86985	0.0	42.636894	15
AACGCAG	87130	0.0	42.63336	14
GGTATCA	88125	0.0	42.261333	8
GTATCAA	88050	0.0	42.17723	9
ATCAACG	88530	0.0	42.142323	11
GTGGTAT	88290	0.0	42.03064	6
TGGTATC	88500	0.0	42.026497	7
CGCAGAG	88665	0.0	41.88468	16
AGTGGTA	88480	0.0	41.88461	5
TATCAAC	88910	0.0	41.76926	10
AGAGTAC	87505	0.0	41.602024	19
CAGAGTA	89870	0.0	40.402637	18
GCAGTGG	93010	0.0	40.259007	3
GAGTACT	43655	0.0	40.22804	20
GCAGAGT	90700	0.0	40.193554	17
AGCAGTG	93195	0.0	40.174046	2
CAGTGGT	93090	0.0	40.040123	4
>>END_MODULE