FastQCFastQC Report
Thu 26 May 2016
SRR805320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18764221
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4279312.2805689615358933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC441240.23514964996415255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG312230.1663964627148657No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG246330.13127643295183958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG215640.11492083790741967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA2313200.045.75855613
TCAACGC2312750.045.75120512
AACGCAG2318650.045.62059814
ACGCAGA2318150.045.6000315
GTATCAA2317150.045.5973979
ATCAACG2329350.045.5744711
GGTATCA2325450.045.5225688
GTGGTAT2321400.045.4835436
AGTGGTA2319350.045.4659965
TGGTATC2329500.045.38397
TATCAAC2328100.045.3829310
AGAGTAC2283050.045.34793519
CGCAGAG2332750.045.33880616
AAGCAGT2355000.045.2616961
CAGAGTA2303200.044.90630718
GAGTACA1600000.044.6367820
GCAGAGT2327900.044.6044717
CAGTGGT2373900.044.5855564
ACATGGG1585300.044.5333424
GTACATG1586150.044.5242922