FastQCFastQC Report
Thu 26 May 2016
SRR805319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21072699
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT964870.45787680068889136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC517350.040.1818912
CAACGCA518900.040.06186713
AAGCAGT521300.039.5934221
GGTATCA525550.039.4208038
AACGCAG528850.039.32589314
ACGCAGA528600.039.27781315
ATCAACG530800.039.1681411
GTATCAA529550.039.1540959
GTGGTAT528250.039.1392336
TGGTATC531300.038.8747447
AGTGGTA533050.038.707445
TATCAAC547250.037.8619510
CGCAGAG548200.037.83491516
AGAGTAC534700.037.7353719
CAGTGGT568700.036.417364
CAGAGTA570650.035.56812718
GCAGTGG587800.035.4219133
AGCAGTG588350.035.0692632
GCAGAGT584300.034.91417317
GTACATG383150.034.3223322
Produced by FastQC (version 0.11.5)