##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21072699
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.57219433542898	37.0	35.0	37.0	33.0	37.0
2	35.57806605599026	37.0	35.0	37.0	33.0	37.0
3	37.23989309580135	39.0	37.0	39.0	34.0	39.0
4	37.28682699828816	39.0	37.0	39.0	34.0	39.0
5	37.30948849978828	39.0	37.0	39.0	34.0	39.0
6	37.246680028979675	39.0	37.0	39.0	34.0	39.0
7	37.13336896237165	39.0	37.0	39.0	33.0	39.0
8	38.041941281465654	40.0	38.0	40.0	34.0	40.0
9	37.96006913969587	40.0	38.0	40.0	33.0	40.0
10	37.8358413888985	40.0	37.0	40.0	33.0	40.0
11	37.877246146779775	40.0	38.0	40.0	33.0	40.0
12	37.74545813993737	40.0	37.0	40.0	33.0	40.0
13	37.84696825024645	40.0	38.0	40.0	33.0	40.0
14	37.71755910336877	40.0	37.0	40.0	33.0	40.0
15	37.69217991487469	40.0	37.0	40.0	33.0	40.0
16	37.5737055324522	40.0	37.0	40.0	32.0	40.0
17	37.44635245822094	40.0	37.0	40.0	32.0	40.0
18	37.43051822645025	40.0	37.0	40.0	32.0	40.0
19	37.402575721316005	39.0	37.0	40.0	32.0	40.0
20	37.201092892751895	39.0	37.0	40.0	32.0	40.0
21	37.15016230241793	39.0	37.0	40.0	32.0	40.0
22	36.981574263458135	39.0	36.0	40.0	31.0	40.0
23	36.873822522686815	39.0	36.0	40.0	31.0	40.0
24	36.87473764039433	39.0	36.0	40.0	31.0	40.0
25	36.81893923507378	39.0	36.0	40.0	31.0	40.0
26	36.700158057589114	39.0	36.0	40.0	30.0	40.0
27	36.541661416983175	39.0	36.0	40.0	30.0	40.0
28	36.43434668715194	39.0	36.0	40.0	30.0	40.0
29	36.311161137925424	39.0	35.0	40.0	30.0	40.0
30	36.2103693029545	38.0	35.0	40.0	30.0	40.0
31	36.084979527302124	38.0	35.0	40.0	30.0	40.0
32	36.006663788060564	38.0	35.0	40.0	29.0	40.0
33	35.925891220673726	38.0	35.0	40.0	29.0	40.0
34	35.936918332103545	38.0	35.0	40.0	29.0	40.0
35	35.9684207514187	38.0	35.0	40.0	29.0	40.0
36	35.94742685785053	38.0	35.0	40.0	29.0	40.0
37	35.922876656663675	38.0	35.0	40.0	29.0	40.0
38	35.88605308698236	38.0	35.0	40.0	29.0	40.0
39	35.84018924201404	38.0	35.0	40.0	29.0	40.0
40	35.794018459619245	38.0	35.0	40.0	28.0	40.0
41	35.74665983697675	38.0	35.0	40.0	28.0	40.0
42	35.49810453800911	38.0	34.0	40.0	27.0	40.0
43	35.52838395309495	38.0	34.0	40.0	27.0	40.0
44	35.41827537137032	38.0	34.0	40.0	27.0	40.0
45	35.2767097845416	38.0	34.0	40.0	27.0	40.0
46	35.14239965179591	38.0	34.0	40.0	27.0	40.0
47	35.3235951408028	38.0	34.0	40.0	27.0	40.0
48	35.39950179139369	38.0	34.0	40.0	27.0	40.0
49	35.31942956144346	38.0	34.0	40.0	27.0	40.0
50	35.22306924234053	38.0	34.0	40.0	27.0	40.0
51	35.08695715722035	38.0	34.0	40.0	26.0	40.0
52	34.891103650272804	38.0	33.0	40.0	26.0	40.0
53	34.427587942104616	37.0	33.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	4.0
11	19.0
12	38.0
13	98.0
14	246.0
15	647.0
16	1579.0
17	3458.0
18	7120.0
19	13713.0
20	23793.0
21	37223.0
22	54922.0
23	77271.0
24	104516.0
25	138391.0
26	178492.0
27	227044.0
28	286553.0
29	354180.0
30	438559.0
31	537478.0
32	664230.0
33	827552.0
34	1059382.0
35	1401332.0
36	1951817.0
37	2905236.0
38	4603310.0
39	5174491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.955398499262007	35.79032282480759	15.736441734397669	22.51783694153274
2	28.812939434099068	27.690600999900393	27.15410114290533	16.34235842309521
3	22.84176791971451	24.927684868464166	27.345025902946745	24.885521308874576
4	21.32048675872037	30.178274743069224	25.075302409055432	23.425936089154977
5	22.877012574421528	30.094251334392432	23.644655105641665	23.384080985544376
6	24.020976145485683	25.38376787899832	28.527181069686424	22.068074905829576
7	21.87090984405937	26.702203642732236	27.419862068926243	24.00702444428215
8	23.05616855249534	25.16829002302932	27.681926268675884	24.093615155799455
9	24.376317433281802	25.58654209410954	26.930793250546596	23.106347222062062
10	22.420511961946595	26.580643514150704	28.636341267912574	22.36250325599013
11	22.356789702163923	28.73657522465442	26.665487890279266	22.24114718290239
12	22.34494499257072	25.869419954226082	28.790346220007223	22.995288833195975
13	22.717208649921872	26.270426963342473	26.134312458029225	24.878051928706427
14	22.672786243470757	28.23078334673693	26.19746526061991	22.898965149172394
15	22.719306150579	27.93958192066427	26.054868434271285	23.286243494485447
16	23.23777319649467	26.033072460248206	25.638761318614193	25.090393024642925
17	25.20675685634764	25.971661247569667	25.798356442143454	23.02322545393924
18	23.04868493589739	25.73825498100647	25.69904310786198	25.514016975234167
19	23.39560300272879	27.999493562737264	25.48332797806299	23.121575456470953
20	25.307408415030274	25.986585771476168	25.73090423775331	22.97510157574025
21	23.395209128171004	27.692428008391328	25.692219112511406	23.22014375092626
22	25.484704166276945	25.883110654216622	25.576462701811476	23.055722477694953
23	23.208023803690264	25.90501102872489	27.97107290338082	22.91589226420403
24	23.198200667128592	27.68669072718212	25.70711990903491	23.407988696654378
25	23.316367779941242	25.909756505324733	25.511070034265664	25.262805680468364
26	23.219526838968278	27.2027660054367	26.613392048166208	22.964315107428813
27	23.279044606483488	25.4830622313734	27.894851058234167	23.34304210390895
28	24.841056193134065	25.84372319843794	26.159762449034176	23.15545815939382
29	24.786582867244487	25.828637328327048	26.38120062361257	23.003579180815898
30	24.707024952048144	25.525918630546567	26.396960351400644	23.370096066004646
31	24.4632118553015	26.015324377764802	26.13529477168539	23.38616899524831
32	23.967285823235077	26.24707447299465	26.428271006006398	23.35736869776387
33	23.869007002852364	25.874402704655914	26.454186053718132	23.80240423877359
34	23.949196066436482	26.103742097773047	26.349619476840623	23.597442358949845
35	23.74355558345896	26.145141635630065	26.612006368999054	23.499296411911924
36	23.774197125864134	25.866107611559393	26.53918228509789	23.820512977478586
37	24.017231964448406	26.116161010034833	26.265586577210637	23.601020448306123
38	23.906225775824918	26.162429406883287	26.561628389415137	23.369716427876657
39	23.906434576795313	25.837079531198164	26.519592957693742	23.73689293431278
40	23.955635678182468	26.173495858314116	26.246291469355683	23.624576994147738
41	23.800719594580645	26.24519051878452	26.542489882287978	23.411600004346855
42	24.061493024695128	25.80493841818744	26.57195929197299	23.561609265144444
43	24.02258960752963	26.172318980117353	26.164028632497434	23.641062779855584
44	23.91787117540093	26.09778652464025	26.509001053922898	23.475341246035924
45	23.914245631278654	25.757113505014235	26.50415592231446	23.82448494139265
46	24.02494336392315	26.055997857702046	26.175669286596843	23.743389491777965
47	23.81758501841648	26.04472260530082	26.547439414381614	23.590252961901083
48	23.817528072697286	25.748486228555723	26.5388501017359	23.89513559701109
49	23.98548472599547	26.128299939177225	26.195903998818565	23.690311336008737
50	23.875294759347156	26.138915570331072	26.49463175077858	23.491157919543195
51	23.886309010535385	25.834545446693845	26.46168865222248	23.817456890548286
52	24.05395720785458	26.099504387169386	26.07706302832874	23.769475376647293
53	23.970531729229368	26.122135565074032	26.251288456215317	23.656044249481283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7138.0
1	4496.0
2	1854.0
3	1564.0
4	1274.0
5	1412.0
6	1550.0
7	1769.5
8	2385.0
9	2781.0
10	3904.0
11	5027.0
12	7315.5
13	9604.0
14	12237.5
15	14871.0
16	20946.5
17	27022.0
18	37629.5
19	48237.0
20	63887.0
21	79537.0
22	156135.0
23	232733.0
24	215603.5
25	227318.0
26	256162.0
27	293223.5
28	330285.0
29	370428.0
30	410571.0
31	449234.5
32	487898.0
33	541615.0
34	595332.0
35	663068.5
36	730805.0
37	801939.0
38	873073.0
39	941703.0
40	1010333.0
41	1079195.0
42	1213341.5
43	1278626.0
44	1335044.0
45	1391462.0
46	1435775.5
47	1480089.0
48	1502112.0
49	1524135.0
50	1532314.5
51	1540494.0
52	1515528.5
53	1490563.0
54	1432314.0
55	1374065.0
56	1288189.5
57	1202314.0
58	1098356.0
59	879082.0
60	763766.0
61	666229.5
62	568693.0
63	483918.0
64	399143.0
65	327280.0
66	255417.0
67	205776.5
68	156136.0
69	121136.5
70	86137.0
71	65388.5
72	44640.0
73	33825.5
74	23011.0
75	17547.0
76	9244.0
77	6405.0
78	4871.5
79	3338.0
80	2572.5
81	1807.0
82	1318.5
83	830.0
84	569.0
85	308.0
86	212.5
87	117.0
88	86.5
89	56.0
90	45.5
91	35.0
92	26.0
93	10.0
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.1072699E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.13807142235974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.90242540245464	42.40251424393235
2	12.616747938172473	13.913262978657276
3	4.1050993204206545	6.790417785757033
4	1.9285245017350632	4.253404868657545
5	1.094880472424505	3.0184798843744662
6	0.6581353262504988	2.177298757462682
7	0.46215629416501425	1.7837684729174557
8	0.326979099789448	1.442319756624758
9	0.2409503806211493	1.1956985366340325
>10	1.5393417796897835	15.88137275635733
>50	0.09698456855030607	3.6273909109274567
>100	0.02669048326001816	2.4476591794440106
>500	8.937897901437692E-4	0.3312406477438891
>1k	1.4739508884174888E-4	0.1552297426476908
>5k	3.459806998024157E-5	0.11977788372329787
>10k+	8.649517495049953E-6	0.46016359413888547
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96487	0.45787680068889136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.738576771774703E-4	0.0	0.0	0.0	1.4236429799524019E-5
2	7.023305367765182E-4	0.0	0.0	0.0	1.4236429799524019E-5
3	7.213124431758837E-4	0.0	0.0	0.0	1.4236429799524019E-5
4	7.40294349575249E-4	4.74547659984134E-6	0.0	0.0	2.37273829992067E-5
5	0.0010107865157662054	4.74547659984134E-6	0.0	0.0	2.37273829992067E-5
6	0.0010250229455657294	4.74547659984134E-6	0.0	0.0	3.321833619888938E-5
7	0.0010297684221655706	4.74547659984134E-6	0.0	0.0	4.2709289398572056E-5
8	0.0010297684221655706	4.74547659984134E-6	0.0	0.0	4.74547659984134E-5
9	0.0010392593753652535	1.898190639936536E-5	0.0	0.0	5.6945719198096074E-5
10	0.0010392593753652535	1.898190639936536E-5	0.0	0.0	6.643667239777876E-5
11	0.0010677322349643015	2.8472859599048037E-5	0.0	0.0	6.643667239777876E-5
12	0.0010772231881639842	2.8472859599048037E-5	0.0	0.0	6.643667239777876E-5
13	0.0011246779541623975	3.796381279873072E-5	0.0	0.0	9.49095319968268E-5
14	0.0011389143839619215	3.796381279873072E-5	0.0	0.0	9.965500859666813E-5
15	0.0011531508137614455	3.796381279873072E-5	0.0	0.0	9.965500859666813E-5
16	0.001157896290361287	4.2709289398572056E-5	0.0	0.0	1.1389143839619215E-4
17	0.001172132720160811	4.2709289398572056E-5	0.0	4.74547659984134E-6	1.3761882139539885E-4
18	0.0011768781967606522	4.2709289398572056E-5	0.0	4.74547659984134E-6	1.3761882139539885E-4
19	0.001186369149960335	4.2709289398572056E-5	0.0	4.74547659984134E-6	1.6134620439460555E-4
20	0.001200605579759859	5.220024259825474E-5	0.0	4.74547659984134E-6	1.7083715759428822E-4
21	0.0012053510563597002	5.220024259825474E-5	0.0	4.74547659984134E-6	1.7083715759428822E-4
22	0.0012243329627590657	5.220024259825474E-5	0.0	4.74547659984134E-6	1.898190639936536E-4
23	0.0012385693925585897	5.220024259825474E-5	0.0	4.74547659984134E-6	1.898190639936536E-4
24	0.0012622967755577964	5.6945719198096074E-5	0.0	4.74547659984134E-6	2.040554937931776E-4
25	0.0012812786819571618	6.169119579793742E-5	0.0	4.74547659984134E-6	2.135464469928603E-4
26	0.001300260588356527	6.643667239777876E-5	0.0	4.74547659984134E-6	2.1829192359270163E-4
27	0.001328733447955575	7.118214899762009E-5	0.0	4.74547659984134E-6	2.2303740019254296E-4
28	0.0013761882139539886	8.067310219730278E-5	0.0	4.74547659984134E-6	2.277828767923843E-4
29	0.0014046610735530365	8.541857879714411E-5	0.0	4.74547659984134E-6	2.4201930659190833E-4
30	0.0014426248863517674	8.541857879714411E-5	0.0	4.74547659984134E-6	2.5625573639143236E-4
31	0.0014995706055498633	8.541857879714411E-5	0.0	1.4236429799524019E-5	2.5625573639143236E-4
32	0.0015612618013478008	8.541857879714411E-5	0.0	1.4236429799524019E-5	2.6574668959111503E-4
33	0.001618207520545897	8.541857879714411E-5	0.0	1.898190639936536E-5	2.7998311939063906E-4
34	0.0016846441929436757	8.541857879714411E-5	0.0	1.898190639936536E-5	2.7998311939063906E-4
35	0.0017510808653414544	8.541857879714411E-5	0.0	1.898190639936536E-5	2.9421954919016304E-4
36	0.0018270084909389158	8.541857879714411E-5	0.0	1.898190639936536E-5	3.0371050238984577E-4
37	0.001874463256937329	8.541857879714411E-5	0.0	1.898190639936536E-5	3.0371050238984577E-4
38	0.001988354695333521	9.016405539698546E-5	0.0	1.898190639936536E-5	3.226924087892111E-4
39	0.0021402099465284443	9.016405539698546E-5	0.0	1.898190639936536E-5	3.4167431518857645E-4
40	0.002249355908324795	9.016405539698546E-5	0.0	1.898190639936536E-5	3.511652683882591E-4
41	0.002377483776520511	9.016405539698546E-5	0.0	1.898190639936536E-5	3.6540169818778315E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	51735	0.0	40.18189	12
CAACGCA	51890	0.0	40.061867	13
AAGCAGT	52130	0.0	39.593422	1
GGTATCA	52555	0.0	39.420803	8
AACGCAG	52885	0.0	39.325893	14
ACGCAGA	52860	0.0	39.277813	15
ATCAACG	53080	0.0	39.16814	11
GTATCAA	52955	0.0	39.154095	9
GTGGTAT	52825	0.0	39.139233	6
TGGTATC	53130	0.0	38.874744	7
AGTGGTA	53305	0.0	38.70744	5
TATCAAC	54725	0.0	37.86195	10
CGCAGAG	54820	0.0	37.834915	16
AGAGTAC	53470	0.0	37.73537	19
CAGTGGT	56870	0.0	36.41736	4
CAGAGTA	57065	0.0	35.568127	18
GCAGTGG	58780	0.0	35.421913	3
AGCAGTG	58835	0.0	35.069263	2
GCAGAGT	58430	0.0	34.914173	17
GTACATG	38315	0.0	34.32233	22
>>END_MODULE