FastQCFastQC Report
Thu 26 May 2016
SRR805318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23092797
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3938601.7055534676029067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC403800.174859719244923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG350380.15172696490598347No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG239390.10366435906399732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2125350.044.5575112
CAACGCA2128250.044.51114713
AGAGTAC2063450.044.40223719
ATCAACG2136350.044.29728311
AACGCAG2140250.044.2462114
ACGCAGA2139750.044.222515
AAGCAGT2132950.044.0781331
GTATCAA2149750.043.853919
GGTATCA2150700.043.842198
AGTGGTA2141450.043.830745
CGCAGAG2156000.043.83033416
GTGGTAT2146750.043.8090136
CAGAGTA2099900.043.7982518
TGGTATC2152900.043.6762287
GTACATG1396400.043.43561622
GAGTACA1419150.043.4182420
TATCAAC2172000.043.40250810
ACATGGG1405100.043.2419424
AGTACAT1416600.043.0534721
CAGTGGT2195350.042.854174
Produced by FastQC (version 0.11.5)