FastQCFastQC Report
Thu 26 May 2016
SRR805317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18399132
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2815771.5303819767149884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG221170.12020675757965103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC220790.12000022609762243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1363850.044.1259312
CAACGCA1364650.044.1052313
GTGGTAT1363500.043.844266
GTATCAA1365000.043.835689
AAGCAGT1367200.043.8338931
AACGCAG1374200.043.78503414
ACGCAGA1374050.043.78126515
GGTATCA1367200.043.7617038
AGTGGTA1364750.043.7076765
ATCAACG1377300.043.68819411
TGGTATC1371950.043.5656557
CGCAGAG1386600.043.3477216
TATCAAC1382300.043.28875710
AGAGTAC1352450.043.17383619
CAGTGGT1398250.042.7445374
GCAGTGG1413300.042.611943
CAGAGTA1379350.042.4988218
GCAGAGT1395050.042.21594217
AGCAGTG1418850.042.2150342
GTACATG877800.041.90003622