##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18399132
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.148184273040705	35.0	33.0	35.0	32.0	36.0
2	34.77239730656859	35.0	35.0	37.0	33.0	37.0
3	37.05345882620985	39.0	37.0	39.0	33.0	39.0
4	37.1185948880632	39.0	37.0	39.0	33.0	39.0
5	37.17283087049976	39.0	37.0	39.0	33.0	39.0
6	37.181683244622626	39.0	37.0	39.0	33.0	39.0
7	37.00496860395371	39.0	37.0	39.0	33.0	39.0
8	37.92684051617218	40.0	38.0	40.0	33.0	40.0
9	37.88016581434385	40.0	38.0	40.0	33.0	40.0
10	37.61212039785355	40.0	37.0	40.0	33.0	40.0
11	37.72624436848434	40.0	37.0	40.0	33.0	40.0
12	37.66732963272398	40.0	37.0	40.0	33.0	40.0
13	37.76095019047638	40.0	37.0	40.0	33.0	40.0
14	37.655679246173136	40.0	37.0	40.0	33.0	40.0
15	37.617505217093935	40.0	37.0	40.0	33.0	40.0
16	37.479870463454475	40.0	37.0	40.0	32.0	40.0
17	37.34911326251695	39.0	37.0	40.0	32.0	40.0
18	37.32102666582315	39.0	37.0	40.0	32.0	40.0
19	37.284982519827565	39.0	37.0	40.0	32.0	40.0
20	37.10646572892678	39.0	37.0	40.0	32.0	40.0
21	36.955406428955456	39.0	36.0	40.0	31.0	40.0
22	36.86191223585982	39.0	36.0	40.0	31.0	40.0
23	36.55025508812046	39.0	36.0	40.0	30.0	40.0
24	36.64845705764815	39.0	36.0	40.0	30.0	40.0
25	36.63627170020847	39.0	36.0	40.0	30.0	40.0
26	36.549417331208886	39.0	36.0	40.0	30.0	40.0
27	36.42306093570066	39.0	36.0	40.0	30.0	40.0
28	36.324965384236606	38.0	35.0	40.0	30.0	40.0
29	36.214226464596265	38.0	35.0	40.0	30.0	40.0
30	36.116332933531865	38.0	35.0	40.0	30.0	40.0
31	35.951659947871455	38.0	35.0	40.0	29.0	40.0
32	35.85595918329191	38.0	35.0	40.0	29.0	40.0
33	35.74243589317148	38.0	35.0	40.0	28.0	40.0
34	35.78001619859024	38.0	35.0	40.0	29.0	40.0
35	35.82022804119238	38.0	35.0	40.0	29.0	40.0
36	35.79683764429757	38.0	35.0	40.0	29.0	40.0
37	35.69482565807996	38.0	34.0	40.0	28.0	40.0
38	35.659006740100565	38.0	34.0	40.0	28.0	40.0
39	35.61150694500154	38.0	34.0	40.0	28.0	40.0
40	35.5600610941864	38.0	34.0	40.0	28.0	40.0
41	35.50394002282282	38.0	34.0	40.0	28.0	40.0
42	35.26580862618954	38.0	34.0	40.0	27.0	40.0
43	35.27304918514634	38.0	34.0	40.0	27.0	40.0
44	35.16416959234816	38.0	34.0	40.0	27.0	40.0
45	35.03188068871945	38.0	33.0	40.0	26.0	40.0
46	34.87336581964845	38.0	33.0	40.0	26.0	40.0
47	35.03619529443019	38.0	33.0	40.0	26.0	40.0
48	35.09615692740288	38.0	34.0	40.0	26.0	40.0
49	34.988508316587975	38.0	33.0	40.0	26.0	40.0
50	34.86427223849473	38.0	33.0	40.0	26.0	40.0
51	34.673528131653164	37.0	33.0	40.0	26.0	40.0
52	34.42788942434893	37.0	33.0	40.0	25.0	40.0
53	33.930310897274936	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	9.0
12	40.0
13	100.0
14	280.0
15	633.0
16	1449.0
17	3222.0
18	6586.0
19	12495.0
20	21561.0
21	34034.0
22	49772.0
23	70141.0
24	96289.0
25	126269.0
26	163880.0
27	209158.0
28	262519.0
29	327311.0
30	406280.0
31	502163.0
32	624267.0
33	787499.0
34	1017438.0
35	1362352.0
36	1891593.0
37	2768443.0
38	4012198.0
39	3641148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.536185511359992	44.19438373505881	26.457829641093937	17.811601112487264
2	28.843741106917438	42.33492645196523	16.256766895307887	12.564565545809444
3	34.46706616377338	25.17673659822648	21.625020136819497	18.731177101180645
4	22.01535920281457	26.26632060686341	20.973315480317222	30.745004710004796
5	19.72372935853713	34.03761112208989	24.843226300023282	21.395433219349698
6	28.569184676755405	24.395851934754315	23.493678940941347	23.54128444754894
7	22.86535582222031	24.508775740072956	30.778321499079414	21.84754693862732
8	27.26156864356427	25.50002902310826	25.31512899630265	21.923273337024813
9	28.54705863298334	24.5509788179138	25.099961237301848	21.802001311801014
10	21.865460827173806	25.64375862948317	31.032757414860658	21.458023128482363
11	21.311554262451075	32.41573569883623	24.839416337683755	21.43329370102894
12	21.400824778038444	25.09629802101534	31.62728546107501	21.875591739871204
13	21.503019816369598	25.569184459353846	24.633292483580206	28.294503240696358
14	21.642124204554865	32.44382941543112	24.456425444417704	21.45762093559631
15	21.559402910963406	32.12058590590034	24.376160788454584	21.943850394681665
16	21.834198482841476	25.161572839414383	24.48989984962334	28.514328828120806
17	28.720034184221298	24.97352592502733	24.564783816975712	21.741656073775655
18	21.852965672511075	24.745841271207794	24.439256156214327	28.96193690006681
19	21.840970541436413	32.046555239671086	24.34601806215641	21.766456156736087
20	28.972459135572265	25.235174137562577	24.372698668611108	21.41966805825405
21	22.233092300223728	31.65313450656259	24.30790213364413	21.805871059569547
22	28.713213210275356	25.093216353901916	24.51942841651443	21.674142019308302
23	22.030588182094675	24.982428518910567	31.439325507311978	21.54765779168278
24	22.161203039360768	31.396943073184104	24.391585429138722	22.050268458316403
25	21.85764524109072	25.165877390302978	24.275944104319706	28.700533264286598
26	22.05061086577345	29.330008611275794	26.96592969711832	21.653450825832437
27	22.266871067613405	24.62865096027356	31.175747855931462	21.92873011618157
28	26.478917592416863	24.919555987749856	26.789790953181924	21.81173546665136
29	26.650566994138636	24.92730635336493	26.73995164554502	21.682175006951415
30	26.732070838993927	24.55622363054953	26.71520591297459	21.996499617481955
31	25.089944460423457	25.554542464285817	26.992767919704036	22.362745155586687
32	24.02874766048746	26.218111810926732	27.328653329950566	22.42448719863524
33	23.941645725461395	25.601332715043295	27.40959736578878	23.04742419370653
34	23.31114315610106	25.853920717564282	27.699355600035915	23.135580526298742
35	23.21997037686343	25.90319478114511	27.94881845513147	22.92801638685999
36	23.438806787189744	25.63345923057675	27.866004765877	23.06172921635651
37	23.533985190170927	25.839186326833246	27.78257691721544	22.844251565780386
38	23.63679438790917	25.82031587142263	27.942894262620648	22.599995478047553
39	23.739000296318327	25.522644220390394	27.790876221769594	22.947479261521686
40	23.32883420804851	26.07696928311618	27.59436151661937	22.999834992215938
41	23.509875357163587	26.19526290696757	27.60207927200044	22.692782463868404
42	23.830602443637012	25.773601711211157	27.597464923888797	22.798330921263023
43	23.463509039448166	26.10270419278475	27.763918428325855	22.66986833944123
44	23.576057827075754	25.907656948164732	27.698404468210786	22.817880756548732
45	23.748305083087615	25.399171004371297	27.624960786193608	23.22756312634748
46	23.461987228527956	25.7659437412591	27.467676192550822	23.304392837662125
47	23.42953461065446	25.7847054958897	27.651309855269258	23.134450038186586
48	23.540827904272877	25.442868717937344	27.617982196116643	23.398321181673136
49	23.21477991461771	26.140309227630954	27.45697460075834	23.187936256992995
50	23.61470638941011	25.982703966687126	27.54001112661184	22.862578517290924
51	23.636604161544142	25.6223010955082	27.52141785819027	23.21967688475739
52	23.693775336793063	25.91704869555803	27.223811427626043	23.165364540022868
53	23.86354965005958	25.938745371249034	27.039107062224453	23.15859791646693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6788.0
1	4045.0
2	1302.0
3	1166.0
4	1030.0
5	1102.5
6	1175.0
7	1356.0
8	1888.5
9	2240.0
10	3127.5
11	4015.0
12	5789.0
13	7563.0
14	9905.5
15	12248.0
16	17907.5
17	23567.0
18	33398.5
19	43230.0
20	59376.0
21	75522.0
22	251115.0
23	426708.0
24	327114.0
25	243077.5
26	258635.0
27	288612.5
28	318590.0
29	348452.0
30	378314.0
31	405423.5
32	432533.0
33	477680.5
34	522828.0
35	583484.0
36	644140.0
37	707665.5
38	771191.0
39	828573.0
40	885955.0
41	942972.5
42	1050447.0
43	1100904.0
44	1149689.5
45	1198475.0
46	1229849.0
47	1261223.0
48	1287878.0
49	1314533.0
50	1312476.0
51	1310419.0
52	1284880.5
53	1259342.0
54	1214424.5
55	1169507.0
56	1107256.0
57	1045005.0
58	943031.5
59	734499.5
60	627941.0
61	542578.5
62	457216.0
63	386522.5
64	315829.0
65	257391.0
66	198953.0
67	158373.0
68	117793.0
69	91258.5
70	64724.0
71	49135.0
72	33546.0
73	25410.5
74	17275.0
75	13070.0
76	6737.5
77	4610.0
78	3519.0
79	2428.0
80	1997.0
81	1566.0
82	1163.5
83	761.0
84	522.5
85	284.0
86	198.5
87	113.0
88	89.5
89	66.0
90	54.5
91	43.0
92	30.0
93	14.0
94	11.0
95	7.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8399132E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.28775213943674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75269903618383	40.13226103236765
2	12.530496687909572	13.103830100028976
3	4.003414160507456	6.279885820083751
4	1.9782911468452231	4.1376158858354035
5	1.1122807770409557	2.90793307896888
6	0.7392793705947669	2.3193153894874783
7	0.5324138803055991	1.9487107506310435
8	0.3712710412630766	1.5530342545691471
9	0.2673859234225666	1.258290800054422
>10	1.548297248127675	14.812546204304066
>50	0.11299681704187081	4.046822681876503
>100	0.04895818970637999	4.283865918903728
>500	0.001566540996709874	0.548145458477374
>1k	5.549979904369013E-4	0.54342607543076
>5k	3.13940213124569E-5	0.11461659399099106
>10k+	6.278804262491377E-5	2.0096999549896943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	281577	1.5303819767149884	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	22117	0.12020675757965103	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	22079	0.12000022609762243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9892714504140737E-4	0.0	0.0	0.0	0.0
2	0.0027283895783779366	0.0	0.0	0.0	1.6305117002258584E-5
3	0.002744694695380195	0.0	0.0	0.0	3.261023400451717E-5
4	0.0033425489854630097	0.0	0.0	5.435039000752862E-6	3.261023400451717E-5
5	0.0033534190634645156	0.0	0.0	1.6305117002258584E-5	5.978542900828148E-5
6	0.0033534190634645156	0.0	0.0	1.6305117002258584E-5	5.978542900828148E-5
7	0.0033588541024652686	5.435039000752862E-6	0.0	1.6305117002258584E-5	6.522046800903433E-5
8	0.0033588541024652686	1.0870078001505724E-5	0.0	1.6305117002258584E-5	6.522046800903433E-5
9	0.0033642891414660215	1.6305117002258584E-5	0.0	1.6305117002258584E-5	7.609054601054006E-5
10	0.003375159219467527	1.6305117002258584E-5	0.0	1.6305117002258584E-5	8.696062401204579E-5
11	0.00338059425846828	1.6305117002258584E-5	0.0	2.1740156003011448E-5	1.0870078001505724E-4
12	0.0034023344144712914	1.6305117002258584E-5	0.0	2.717519500376431E-5	1.0870078001505724E-4
13	0.00345668480447882	1.6305117002258584E-5	5.435039000752862E-6	3.261023400451717E-5	1.3044093601806867E-4
14	0.0034621198434795727	1.6305117002258584E-5	5.435039000752862E-6	3.261023400451717E-5	1.3587597501882155E-4
15	0.0035219052724878544	1.6305117002258584E-5	5.435039000752862E-6	3.804527300527003E-5	1.413110140195744E-4
16	0.003538210389490113	1.6305117002258584E-5	5.435039000752862E-6	3.804527300527003E-5	1.413110140195744E-4
17	0.003543645428490866	1.6305117002258584E-5	5.435039000752862E-6	4.3480312006022896E-5	1.521810920210801E-4
18	0.0036251710135021585	1.6305117002258584E-5	5.435039000752862E-6	4.3480312006022896E-5	1.57616131021833E-4
19	0.0036360410915036645	1.6305117002258584E-5	5.435039000752862E-6	4.3480312006022896E-5	1.7935628702484444E-4
20	0.003657781247506676	1.6305117002258584E-5	5.435039000752862E-6	4.3480312006022896E-5	2.0653148202860874E-4
21	0.0036849564425104403	1.6305117002258584E-5	5.435039000752862E-6	4.3480312006022896E-5	2.0653148202860874E-4
22	0.0037012615595126987	1.6305117002258584E-5	5.435039000752862E-6	4.3480312006022896E-5	2.2827163803162018E-4
23	0.003728436754516463	2.1740156003011448E-5	5.435039000752862E-6	4.891535100677576E-5	2.445767550338788E-4
24	0.0037447418715187216	2.1740156003011448E-5	5.435039000752862E-6	4.891535100677576E-5	2.6088187203613734E-4
25	0.003777352105523239	2.1740156003011448E-5	5.435039000752862E-6	4.891535100677576E-5	2.8805706703990166E-4
26	0.0037882221835247444	3.261023400451717E-5	5.435039000752862E-6	4.891535100677576E-5	3.043621840421602E-4
27	0.0038208324175292617	3.261023400451717E-5	5.435039000752862E-6	4.891535100677576E-5	3.261023400451717E-4
28	0.0038751828075367905	3.261023400451717E-5	5.435039000752862E-6	4.891535100677576E-5	3.4784249604818317E-4
29	0.004005623743554859	3.804527300527003E-5	5.435039000752862E-6	4.891535100677576E-5	3.53277535048936E-4
30	0.004081714289565399	4.3480312006022896E-5	5.435039000752862E-6	4.891535100677576E-5	3.6414761305044173E-4
31	0.004163239874576692	4.3480312006022896E-5	5.435039000752862E-6	4.891535100677576E-5	3.695826520511946E-4
32	0.004261070576590244	4.891535100677576E-5	5.435039000752862E-6	4.891535100677576E-5	3.8588776905345315E-4
33	0.0043752063956060535	4.891535100677576E-5	5.435039000752862E-6	5.435039000752862E-5	4.1849800305797033E-4
34	0.004489342214621864	4.891535100677576E-5	5.435039000752862E-6	5.435039000752862E-5	4.1849800305797033E-4
35	0.0046469583456436965	4.891535100677576E-5	5.435039000752862E-6	5.978542900828148E-5	4.3480312006022895E-4
36	0.004750224086658001	4.891535100677576E-5	5.435039000752862E-6	5.978542900828148E-5	4.7828343206625184E-4
37	0.004902405178679081	4.891535100677576E-5	5.435039000752862E-6	5.978542900828148E-5	4.7828343206625184E-4
38	0.005005670919693386	4.891535100677576E-5	5.435039000752862E-6	5.978542900828148E-5	4.7828343206625184E-4
39	0.0051361118557114544	4.891535100677576E-5	5.435039000752862E-6	5.978542900828148E-5	4.891535100677575E-4
40	0.005233942557725006	4.891535100677576E-5	5.435039000752862E-6	7.06555070097872E-5	5.054586270700161E-4
41	0.005407863805749097	4.891535100677576E-5	5.435039000752862E-6	8.152558501129292E-5	5.217637440722747E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	136385	0.0	44.12593	12
CAACGCA	136465	0.0	44.10523	13
GTGGTAT	136350	0.0	43.84426	6
GTATCAA	136500	0.0	43.83568	9
AAGCAGT	136720	0.0	43.833893	1
AACGCAG	137420	0.0	43.785034	14
ACGCAGA	137405	0.0	43.781265	15
GGTATCA	136720	0.0	43.761703	8
AGTGGTA	136475	0.0	43.707676	5
ATCAACG	137730	0.0	43.688194	11
TGGTATC	137195	0.0	43.565655	7
CGCAGAG	138660	0.0	43.34772	16
TATCAAC	138230	0.0	43.288757	10
AGAGTAC	135245	0.0	43.173836	19
CAGTGGT	139825	0.0	42.744537	4
GCAGTGG	141330	0.0	42.61194	3
CAGAGTA	137935	0.0	42.49882	18
GCAGAGT	139505	0.0	42.215942	17
AGCAGTG	141885	0.0	42.215034	2
GTACATG	87780	0.0	41.900036	22
>>END_MODULE