FastQCFastQC Report
Thu 26 May 2016
SRR805316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12392485
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT554620.4475454277330172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135820.1095986801678598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC228900.037.65749412
CAACGCA229400.037.63687513
AACGCAG234200.036.84543214
ACGCAGA234750.036.75910615
AAGCAGT237750.036.0778121
CGCAGAG241000.035.76680816
ATCAACG241300.035.7320811
GGTATCA241500.035.4884038
AGAGTAC238000.035.14138819
GTGGTAT244500.035.024136
AGTGGTA244700.034.9474875
TGGTATC245050.034.916757
GTATCAA246600.034.8211679
TATCAAC252550.033.95426610
GAGTACT113300.032.95807620
CAGTGGT264800.032.4189954
CAGAGTA259300.032.40879418
GCAGTGG267900.032.2456133
GCAGAGT262800.032.1828417
AGCAGTG269150.031.9212342
Produced by FastQC (version 0.11.5)