##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12392485
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.95480753053161	35.0	35.0	37.0	32.0	37.0
2	35.226425854055904	35.0	35.0	37.0	33.0	37.0
3	36.96256949272079	39.0	37.0	39.0	33.0	39.0
4	37.001691912477604	39.0	37.0	39.0	33.0	39.0
5	37.058913284946485	39.0	37.0	39.0	33.0	39.0
6	37.01591319255178	39.0	37.0	39.0	33.0	39.0
7	36.94306928755613	39.0	37.0	39.0	33.0	39.0
8	37.78996601569419	40.0	37.0	40.0	33.0	40.0
9	37.76313717547369	40.0	37.0	40.0	33.0	40.0
10	37.629867940126616	40.0	37.0	40.0	33.0	40.0
11	37.633749324691536	40.0	37.0	40.0	33.0	40.0
12	37.581676717785015	40.0	37.0	40.0	33.0	40.0
13	37.68271553284107	40.0	37.0	40.0	33.0	40.0
14	37.58235067462256	40.0	37.0	40.0	32.0	40.0
15	37.501226025288716	40.0	37.0	40.0	32.0	40.0
16	37.42246724527001	40.0	37.0	40.0	32.0	40.0
17	37.30819153704846	39.0	37.0	40.0	32.0	40.0
18	37.265406736421305	39.0	37.0	40.0	32.0	40.0
19	37.1903085620035	39.0	36.0	40.0	32.0	40.0
20	37.00979892249214	39.0	36.0	40.0	31.0	40.0
21	36.92426902271821	39.0	36.0	40.0	31.0	40.0
22	36.82959309613851	39.0	36.0	40.0	31.0	40.0
23	36.707878766849426	39.0	36.0	40.0	30.0	40.0
24	36.69291477859364	39.0	36.0	40.0	30.0	40.0
25	36.65914786259576	39.0	36.0	40.0	30.0	40.0
26	36.52608149213011	39.0	36.0	40.0	30.0	40.0
27	36.46354641542838	39.0	36.0	40.0	30.0	40.0
28	36.31130673145862	39.0	35.0	40.0	30.0	40.0
29	36.2373878201184	38.0	35.0	40.0	30.0	40.0
30	36.12248753982757	38.0	35.0	40.0	30.0	40.0
31	36.00308816189812	38.0	35.0	40.0	29.0	40.0
32	35.850028222749515	38.0	35.0	40.0	29.0	40.0
33	35.77174279412079	38.0	35.0	40.0	29.0	40.0
34	35.68524585666233	38.0	34.0	40.0	28.0	40.0
35	35.570589353144264	38.0	34.0	40.0	27.0	40.0
36	35.491259581915976	38.0	34.0	40.0	27.0	40.0
37	35.40958096782042	38.0	34.0	40.0	27.0	40.0
38	35.35339312494629	38.0	34.0	40.0	27.0	40.0
39	35.27534647005827	38.0	34.0	40.0	27.0	40.0
40	35.40805125041507	38.0	34.0	40.0	27.0	40.0
41	35.456161375220546	38.0	34.0	40.0	27.0	40.0
42	35.403362037557436	38.0	34.0	40.0	27.0	40.0
43	35.38510250365443	38.0	34.0	40.0	27.0	40.0
44	35.264064713413006	38.0	34.0	40.0	27.0	40.0
45	35.1076674290911	38.0	33.0	40.0	26.0	40.0
46	35.006039224578444	38.0	33.0	40.0	26.0	40.0
47	34.91242539329279	38.0	33.0	40.0	26.0	40.0
48	34.858502067987175	38.0	33.0	40.0	26.0	40.0
49	34.68547083171777	37.0	33.0	40.0	26.0	40.0
50	34.533833206172936	37.0	33.0	40.0	25.0	40.0
51	34.373322057682536	37.0	33.0	40.0	25.0	40.0
52	34.16714065016016	37.0	32.0	39.0	24.0	40.0
53	34.016378878005504	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	13.0
12	39.0
13	79.0
14	232.0
15	619.0
16	1486.0
17	3244.0
18	6250.0
19	11063.0
20	17952.0
21	27137.0
22	38697.0
23	53587.0
24	71446.0
25	93429.0
26	118695.0
27	148921.0
28	186537.0
29	229930.0
30	280583.0
31	342262.0
32	421579.0
33	526807.0
34	672166.0
35	882638.0
36	1216752.0
37	1779342.0
38	2730428.0
39	2530569.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.648587833675005	30.11526743829022	24.41520001839825	23.820944709636528
2	23.4060400315191	25.50108392303884	30.214690596760857	20.878185448681197
3	20.913755392885285	26.676594726562108	28.502717574400936	23.90693230615167
4	21.18531513251781	28.58390387400106	27.784734054549997	22.446046938931136
5	23.907150180129328	29.249403973456495	26.204607066298646	20.638838780115528
6	23.8638981608612	26.79341552561895	27.942628133098406	21.400058180421443
7	22.491074227646838	27.622934383216922	28.537972811748407	21.34801857738783
8	22.85752211925211	26.66738753365447	28.08674773461497	22.38834261247845
9	22.87040089215359	26.5972079046293	28.502297965258784	22.030093237958326
10	21.701765223036382	27.308316290074185	29.18981140586412	21.800107081025317
11	21.7598568810049	28.682092413264975	27.735760826016737	21.82228987971339
12	21.72357682902178	27.0403070893368	28.97124345924163	22.264872622399785
13	21.958114131265845	27.301957597689245	27.316030642764545	23.423897628280365
14	21.976899709783794	28.611130051801553	27.302708052501174	22.10926218591348
15	22.067123744753374	28.460901909504027	27.214235078759426	22.257739266983176
16	22.250605911566566	27.08870739000289	27.06050481400623	23.60018188442431
17	23.788957581953902	27.00439823005636	27.00903813883979	22.197606049149947
18	22.224097910951677	26.907549212284703	27.010208202793873	23.858144673969747
19	22.542694221538294	28.386768271254713	26.863804959215205	22.206732547991788
20	23.865544319803494	26.911866344804935	26.99150331834172	22.23108601704985
21	22.48958945683614	28.191553187274383	26.968077831040343	22.35077952484913
22	23.952032219526593	26.83663526726076	26.992205356714173	22.219127156498473
23	22.419982755678138	26.891902632926325	28.526715989569485	22.161398621826052
24	22.342532591324503	28.250968227922	26.918814103870208	22.487685076883288
25	22.385445695516275	26.874004689132164	26.990397809640278	23.750151805711283
26	22.487055663170057	27.529224364604836	27.794506105918227	22.18921386630688
27	22.481963867618155	26.696719826572313	28.418965203508417	22.402351102301115
28	23.401819731877826	26.82593523413585	27.51745916981138	22.25478586417494
29	23.44871912292006	26.78552364598384	27.525649617489954	22.24010761360615
30	23.320697987530345	26.70716163868667	27.4492888230246	22.522851550758382
31	23.129469190400474	26.963994711310928	27.457584172988707	22.44895192529989
32	22.97883757777395	27.070591572231073	27.535292558352904	22.41527829164207
33	22.97671532384344	26.93103118543214	27.418463689889478	22.673789800834943
34	22.922416286967465	27.084995462976153	27.4055042229222	22.587084027134186
35	22.853503554775333	27.08371242732995	27.520307670334077	22.54247634756064
36	22.86533330482143	27.006714149744788	27.427703160423434	22.700249385010352
37	22.998066973653792	27.14110204692602	27.247811879538286	22.613019099881903
38	22.973414936552274	27.149340911044074	27.411548208450526	22.46569594395313
39	22.93781271472187	27.041436806257984	27.378148934616426	22.642601544403725
40	22.890445298098	27.153633835344564	27.361816455698758	22.59410441085868
41	22.82739095508286	27.182433547428136	27.468550496530757	22.52162500095824
42	22.826801888402528	27.053427944435683	27.47568385194737	22.644086315214423
43	22.92419962582162	27.12251013416599	27.325689722440654	22.62760051757174
44	22.887612936388464	27.026040378503584	27.50980130296708	22.57654538214087
45	22.90355001438372	26.904208477960633	27.50517753299681	22.687063974658837
46	22.93761904896395	27.015106332587855	27.37181445045122	22.675460167996974
47	22.906196779741915	27.020819472446405	27.444624706021433	22.628359041790247
48	22.84776620669704	26.974549495117405	27.402413640202106	22.775270657983448
49	22.94144394768281	27.190640133919867	27.246803203715803	22.62111271468152
50	22.967879323638478	27.124826053854413	27.3526980262635	22.554596596243613
51	22.937304342107335	27.002889251025923	27.351390782397555	22.708415624469183
52	23.04449833911439	27.13960113730216	27.151979606995692	22.663920916587756
53	23.058208260893597	27.105903295424604	27.18945393115263	22.646434512529165
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16094.0
1	9682.5
2	3271.0
3	2982.5
4	2694.0
5	2793.0
6	2892.0
7	3154.5
8	3874.5
9	4332.0
10	5391.0
11	6450.0
12	8487.0
13	10524.0
14	13117.5
15	15711.0
16	22488.0
17	29265.0
18	39902.5
19	50540.0
20	65455.0
21	80370.0
22	131892.5
23	183415.0
24	178635.5
25	206390.5
26	238925.0
27	269665.0
28	300405.0
29	324247.5
30	348090.0
31	365673.5
32	383257.0
33	410388.5
34	437520.0
35	474906.5
36	512293.0
37	551416.0
38	590539.0
39	623045.0
40	655551.0
41	684531.0
42	734109.0
43	754707.0
44	769277.0
45	783847.0
46	794667.0
47	805487.0
48	805616.5
49	805746.0
50	800577.0
51	795408.0
52	776492.5
53	757577.0
54	723279.0
55	688981.0
56	642218.0
57	595455.0
58	540149.0
59	429229.5
60	373616.0
61	330105.0
62	286594.0
63	245607.0
64	204620.0
65	166922.0
66	129224.0
67	104151.0
68	79078.0
69	61308.5
70	43539.0
71	32421.0
72	21303.0
73	15655.5
74	10008.0
75	7426.0
76	3584.0
77	2324.0
78	1738.5
79	1153.0
80	903.5
81	654.0
82	505.0
83	356.0
84	237.5
85	119.0
86	79.5
87	40.0
88	29.0
89	18.0
90	16.5
91	15.0
92	10.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.2392485E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.75347549195041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80241250510832	39.13382696348204
2	15.19919776714968	16.340194093475752
3	5.443786641628103	8.778673554724897
4	2.2534094038996573	4.845143486634032
5	1.1740696520694478	3.15551621341789
6	0.6601921738769451	2.1292574303083094
7	0.45606612773101657	1.7160597593788545
8	0.3179646606678173	1.3673364475611076
9	0.2454758188684076	1.1875660572068463
>10	1.3056249411659353	12.231867786126296
>50	0.08607340708475573	3.2501703621944933
>100	0.055280413797449426	5.036933355132652
>500	2.7766392414648236E-4	0.0967133395601173
>1k	1.3812817634990907E-4	0.1611436939182814
>5k	0.0	0.0
>10k+	3.069485202741605E-5	0.5695974568785003
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55462	0.4475454277330172	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13582	0.1095986801678598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.92233801372364E-4	0.0	0.0	0.0	8.06940657987482E-6
2	5.003032079522388E-4	0.0	0.0	0.0	8.06940657987482E-6
3	5.003032079522388E-4	0.0	0.0	0.0	8.06940657987482E-6
4	5.083726145321137E-4	0.0	0.0	0.0	8.06940657987482E-6
5	5.083726145321137E-4	0.0	0.0	0.0	8.06940657987482E-6
6	5.245114276918632E-4	0.0	0.0	0.0	1.613881315974964E-5
7	5.245114276918632E-4	0.0	0.0	0.0	2.420821973962446E-5
8	5.325808342717381E-4	0.0	0.0	8.06940657987482E-6	4.841643947924892E-5
9	5.487196474314877E-4	0.0	0.0	8.06940657987482E-6	6.455525263899856E-5
10	5.487196474314877E-4	0.0	0.0	8.06940657987482E-6	8.069406579874819E-5
11	5.648584605912374E-4	0.0	0.0	8.06940657987482E-6	9.683287895849783E-5
12	5.971360869107366E-4	0.0	0.0	8.06940657987482E-6	9.683287895849783E-5
13	6.455525263899855E-4	0.0	0.0	8.06940657987482E-6	1.1297169211824747E-4
14	7.181771856088589E-4	0.0	0.0	8.06940657987482E-6	1.1297169211824747E-4
15	8.069406579874819E-4	0.0	0.0	8.06940657987482E-6	1.2104109869812229E-4
16	8.876347237862302E-4	0.0	0.0	8.06940657987482E-6	1.4524931843774676E-4
17	0.0010409534488038518	0.0	0.0	8.06940657987482E-6	1.4524931843774676E-4
18	0.0011539251409220992	0.0	0.0	8.06940657987482E-6	1.5331872501762157E-4
19	0.0013314520856793452	0.0	0.0	8.06940657987482E-6	1.694575381773712E-4
20	0.0014605625909573422	0.0	0.0	8.06940657987482E-6	1.7752694475724602E-4
21	0.0016622977554542127	0.0	0.0	8.06940657987482E-6	1.9366575791699567E-4
22	0.0018398247002114588	0.0	0.0	8.06940657987482E-6	2.098045710767453E-4
23	0.002049629271288204	0.0	0.0	8.06940657987482E-6	2.1787397765662012E-4
24	0.0023804749410630717	0.0	0.0	8.06940657987482E-6	2.2594338423649493E-4
25	0.0024934466331813193	0.0	0.0	8.06940657987482E-6	2.2594338423649493E-4
26	0.0027113206108379394	0.0	0.0	8.06940657987482E-6	2.3401279081636977E-4
27	0.002985680434553683	0.0	0.0	8.06940657987482E-6	2.4208219739624458E-4
28	0.003138999159571305	0.0	0.0	8.06940657987482E-6	2.4208219739624458E-4
29	0.0034779142359260473	8.06940657987482E-6	0.0	1.613881315974964E-5	2.4208219739624458E-4
30	0.003736135246482041	8.06940657987482E-6	0.0	1.613881315974964E-5	2.501516039761194E-4
31	0.003954009224138661	8.06940657987482E-6	0.0	1.613881315974964E-5	2.582210105559942E-4
32	0.004357479553132403	8.06940657987482E-6	0.0	1.613881315974964E-5	2.6629041713586906E-4
33	0.0047690192887060185	8.06940657987482E-6	0.0	1.613881315974964E-5	2.7435982371574384E-4
34	0.005091795551901011	8.06940657987482E-6	0.0	1.613881315974964E-5	2.985680434553683E-4
35	0.005503335287474626	8.06940657987482E-6	0.0	1.613881315974964E-5	2.985680434553683E-4
36	0.005931013836207992	8.06940657987482E-6	0.0	1.613881315974964E-5	2.985680434553683E-4
37	0.006390970011260857	8.06940657987482E-6	0.0	1.613881315974964E-5	3.1470685661511797E-4
38	0.006907412032372846	8.06940657987482E-6	0.0	1.613881315974964E-5	3.2277626319499275E-4
39	0.0072221188889879636	8.06940657987482E-6	0.0	2.420821973962446E-5	3.389150763547424E-4
40	0.00758524218508233	8.06940657987482E-6	0.0	2.420821973962446E-5	3.389150763547424E-4
41	0.007956434887756571	8.06940657987482E-6	0.0	2.420821973962446E-5	3.469844829346172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	22890	0.0	37.657494	12
CAACGCA	22940	0.0	37.636875	13
AACGCAG	23420	0.0	36.845432	14
ACGCAGA	23475	0.0	36.759106	15
AAGCAGT	23775	0.0	36.077812	1
CGCAGAG	24100	0.0	35.766808	16
ATCAACG	24130	0.0	35.73208	11
GGTATCA	24150	0.0	35.488403	8
AGAGTAC	23800	0.0	35.141388	19
GTGGTAT	24450	0.0	35.02413	6
AGTGGTA	24470	0.0	34.947487	5
TGGTATC	24505	0.0	34.91675	7
GTATCAA	24660	0.0	34.821167	9
TATCAAC	25255	0.0	33.954266	10
GAGTACT	11330	0.0	32.958076	20
CAGTGGT	26480	0.0	32.418995	4
CAGAGTA	25930	0.0	32.408794	18
GCAGTGG	26790	0.0	32.245613	3
GCAGAGT	26280	0.0	32.18284	17
AGCAGTG	26915	0.0	31.921234	2
>>END_MODULE