FastQCFastQC Report
Thu 26 May 2016
SRR805315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12335791
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT868420.7039840412341616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC405900.041.8646212
CAACGCA407650.041.684913
AAGCAGT412200.041.2988851
AACGCAG411350.041.25282714
ACGCAGA411950.041.14710615
ATCAACG413700.041.05825411
GGTATCA411200.040.9935578
GTATCAA411850.040.9345639
GTGGTAT414100.040.7575456
AGTGGTA417350.040.4120035
TGGTATC417600.040.3934367
CGCAGAG423750.039.9680216
AGAGTAC413500.039.85054419
TATCAAC423500.039.84734310
CAGTGGT440350.038.472014
CAGAGTA431700.038.32290618
GCAGTGG449050.037.946443
AGCAGTG448850.037.931942
GCAGAGT442050.037.60106317
GAGTACT162000.037.19382520
Produced by FastQC (version 0.11.5)