##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12335791
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.94805886383775	35.0	35.0	37.0	32.0	37.0
2	35.352908621749506	36.0	35.0	37.0	33.0	37.0
3	37.10068490946385	39.0	37.0	39.0	33.0	39.0
4	37.151117022005316	39.0	37.0	39.0	33.0	39.0
5	37.1536948866919	39.0	37.0	39.0	33.0	39.0
6	37.117894101805064	39.0	37.0	39.0	33.0	39.0
7	37.00338065066116	39.0	37.0	39.0	33.0	39.0
8	37.87674126450424	40.0	38.0	40.0	33.0	40.0
9	37.86456758224908	40.0	38.0	40.0	33.0	40.0
10	37.669817201021	40.0	37.0	40.0	33.0	40.0
11	37.695109134063635	40.0	37.0	40.0	33.0	40.0
12	37.65353085181161	40.0	37.0	40.0	33.0	40.0
13	37.764006458929146	40.0	37.0	40.0	33.0	40.0
14	37.71367300240414	40.0	37.0	40.0	33.0	40.0
15	37.616580404126495	40.0	37.0	40.0	33.0	40.0
16	37.5006459658728	40.0	37.0	40.0	32.0	40.0
17	37.40450628581499	39.0	37.0	40.0	32.0	40.0
18	37.34797809074424	39.0	37.0	40.0	32.0	40.0
19	37.274972800690286	39.0	37.0	40.0	32.0	40.0
20	37.119207840016095	39.0	37.0	40.0	32.0	40.0
21	37.02014876873319	39.0	36.0	40.0	31.0	40.0
22	36.931099351472476	39.0	36.0	40.0	31.0	40.0
23	36.75398902267394	39.0	36.0	40.0	30.0	40.0
24	36.74174497606193	39.0	36.0	40.0	30.0	40.0
25	36.72008872394158	39.0	36.0	40.0	30.0	40.0
26	36.604216705681864	39.0	36.0	40.0	30.0	40.0
27	36.54870182220176	39.0	36.0	40.0	30.0	40.0
28	36.38225307157036	39.0	35.0	40.0	30.0	40.0
29	36.33878605757831	38.0	35.0	40.0	30.0	40.0
30	36.20849550709801	38.0	35.0	40.0	30.0	40.0
31	36.10105359275299	38.0	35.0	40.0	30.0	40.0
32	35.9495621318487	38.0	35.0	40.0	29.0	40.0
33	35.824655022122215	38.0	35.0	40.0	29.0	40.0
34	35.75381327391166	38.0	35.0	40.0	28.0	40.0
35	35.60800867978389	38.0	34.0	40.0	28.0	40.0
36	35.47451687532644	38.0	34.0	40.0	27.0	40.0
37	35.40937074890455	38.0	34.0	40.0	27.0	40.0
38	35.34263218305174	38.0	34.0	40.0	27.0	40.0
39	35.269437687457575	38.0	34.0	40.0	27.0	40.0
40	35.4204782652365	38.0	34.0	40.0	27.0	40.0
41	35.48040916062861	38.0	34.0	40.0	28.0	40.0
42	35.440260701563446	38.0	34.0	40.0	27.0	40.0
43	35.40708650138447	38.0	34.0	40.0	27.0	40.0
44	35.26768019983477	38.0	34.0	40.0	27.0	40.0
45	35.10726365256999	38.0	33.0	40.0	27.0	40.0
46	35.03151155852105	38.0	33.0	40.0	26.0	40.0
47	34.897247853826315	38.0	33.0	40.0	26.0	40.0
48	34.834341632409306	37.0	33.0	40.0	26.0	40.0
49	34.640547655192925	37.0	33.0	40.0	26.0	40.0
50	34.47747679901516	37.0	33.0	40.0	26.0	40.0
51	34.287395433337025	37.0	33.0	39.0	25.0	40.0
52	34.05214541977892	37.0	32.0	39.0	25.0	40.0
53	33.8876441729598	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	15.0
12	32.0
13	79.0
14	218.0
15	479.0
16	1159.0
17	2411.0
18	4961.0
19	8941.0
20	14950.0
21	23751.0
22	34946.0
23	49254.0
24	66991.0
25	87392.0
26	113089.0
27	143044.0
28	180378.0
29	222213.0
30	272660.0
31	335061.0
32	414729.0
33	520728.0
34	669545.0
35	892047.0
36	1245245.0
37	1829874.0
38	2742308.0
39	2459287.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.461345527011606	32.536583993681475	23.7274366921424	25.274633787164518
2	26.903730778188446	27.641210847362768	25.299707169163295	20.1553512052855
3	24.013158134731693	24.74741992629415	26.024533003193717	25.214888935780444
4	21.212000106032924	28.110025534641437	24.740837454201355	25.937136905124287
5	24.454272936368653	29.552405678727855	24.384013963920108	21.60930742098338
6	26.32805630380735	25.366820822434494	25.773936993582332	22.531185880175826
7	23.495882833942304	26.161711073088057	28.267194215595904	22.075211877373732
8	24.760755106826956	25.39627981699755	26.51482989619393	23.32813517998157
9	24.97074569437825	25.014528861586584	26.98879220635304	23.02593323768212
10	22.44532190923144	25.96134289240147	28.819724653246798	22.77361054512029
11	22.41283108638919	28.808756568589722	26.09699694166349	22.681415403357597
12	22.371877085141925	25.704221156146374	28.802814509422213	23.121087249289488
13	22.591133393878025	25.983011547455693	25.699251876105876	25.726603182560403
14	22.76907090919423	28.770275047623617	25.5993150337907	22.861339009391454
15	22.712609187363828	28.594793799603124	25.54171840297878	23.150878610054274
16	22.93996388233231	25.78719110918789	25.44667788226957	25.82616712621023
17	25.99115857264443	25.578862352645242	25.421361305489043	23.008617769221285
18	22.930706267640236	25.518890519464865	25.417664744806395	26.132738468088508
19	23.121938431025622	28.506368176957604	25.350348429217064	23.021344962799713
20	26.023479159139452	25.597280304116698	25.40692364194562	22.972316894798233
21	23.230460049136695	28.26423534575124	25.393199349762007	23.112105255350063
22	26.008068716469012	25.515145319825862	25.46241258464901	23.014373379056114
23	23.159398533908366	25.51390502643892	28.43240453733368	22.894291902319033
24	23.059721099360388	28.249562593918785	25.44747231855663	23.243243988164195
25	23.02695465576549	25.625426046858284	25.417632318835494	25.929986978540736
26	23.17055306789812	27.256517235092588	26.669218050143684	22.903711646865613
27	23.212512274243295	25.308121708611957	28.349102218090433	23.130263799054312
28	25.058506584620314	25.489950340436213	26.41607660181662	23.035466473126856
29	25.196114298629084	25.447366934151205	26.433043491090274	22.923475276129434
30	25.08398529125534	25.304441360914755	26.366789126047934	23.244784221781966
31	24.582160965600018	25.702826839397652	26.42449924775801	23.290512947244324
32	24.21878742919688	25.936172232489994	26.55635135193195	23.288688986381175
33	24.107550136022894	25.68063936880902	26.571088955706205	23.64072153946188
34	23.940742835218266	25.838861893817754	26.593981691161922	23.62641357980206
35	23.87598006483735	25.85271588988497	26.745864938859615	23.525439106418062
36	23.867168307245155	25.744388827599302	26.709012822931257	23.67943004222429
37	24.070633168152735	25.875373537051654	26.535469026672065	23.518524268123546
38	24.12205265150812	25.904386674514836	26.647265667844085	23.326295006132966
39	24.07949356470128	25.73266683911879	26.62378115841943	23.564058437760497
40	23.900988594894322	25.997951813548077	26.550765978444353	23.550293613113258
41	23.93316326452029	26.053756909467747	26.672647096566404	23.340432729445563
42	23.89092033092973	25.8913352212274	26.699965977050034	23.517778470792834
43	23.88327590828995	25.98259811632671	26.65746363569227	23.476662339691064
44	23.997796331017607	25.789817612830827	26.75388226016475	23.458503795986815
45	24.01233127247373	25.56391398006014	26.73543998921512	23.68831475825101
46	23.951378553673617	25.796278487532742	26.516402555782598	23.735940403011043
47	23.91952814375665	25.733793641607576	26.682666721574645	23.66401149306113
48	23.804213284741934	25.626771724650656	26.708720989193154	23.86029400141426
49	23.833299380639637	26.00361825196293	26.453812325452013	23.709270041945427
50	24.03742086745795	25.93344845093436	26.56357423695003	23.46555644465766
51	24.002060346191016	25.710584752935585	26.61655016690863	23.670804733964772
52	24.109090369640665	25.863862317381997	26.36211978623827	23.664927526739064
53	24.197815932517013	25.856988011551103	26.346603959162408	23.598592096769476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6399.0
1	3709.0
2	1019.0
3	917.0
4	815.0
5	847.0
6	879.0
7	1054.5
8	1412.5
9	1595.0
10	2235.0
11	2875.0
12	4086.5
13	5298.0
14	6632.0
15	7966.0
16	11491.0
17	15016.0
18	20804.5
19	26593.0
20	36293.0
21	45993.0
22	107774.0
23	169555.0
24	143959.5
25	133504.0
26	148644.0
27	168792.5
28	188941.0
29	211675.0
30	234409.0
31	255410.5
32	276412.0
33	306270.0
34	336128.0
35	373452.0
36	410776.0
37	451383.5
38	491991.0
39	529666.5
40	567342.0
41	606528.0
42	684172.0
43	722630.0
44	759970.5
45	797311.0
46	825496.0
47	853681.0
48	871415.0
49	889149.0
50	897628.5
51	906108.0
52	895753.0
53	885398.0
54	854605.0
55	823812.0
56	780126.0
57	736440.0
58	669765.0
59	531982.0
60	460874.0
61	403887.5
62	346901.0
63	296831.5
64	246762.0
65	203076.0
66	159390.0
67	127706.0
68	96022.0
69	74447.0
70	52872.0
71	39533.5
72	26195.0
73	19502.0
74	12809.0
75	9646.5
76	4829.5
77	3175.0
78	2252.5
79	1330.0
80	1058.0
81	786.0
82	581.5
83	377.0
84	250.5
85	124.0
86	97.5
87	71.0
88	51.0
89	31.0
90	19.0
91	7.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.2335791E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.40915440209853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21229799190873	42.99071284764324
2	13.223908948450156	14.918990433448345
3	4.311369789674633	7.296021724508983
4	1.898832765069022	4.284462025141686
5	1.0747562834367972	3.031304656850593
6	0.6613771408801576	2.238463515475637
7	0.445008308736099	1.7571779678397939
8	0.3317425582830492	1.4970653753548557
9	0.24046935697997568	1.2208205778171117
>10	1.5217976866799103	15.359229671256386
>50	0.059382955567797525	2.1958290124967696
>100	0.018406392646642417	1.9101736398243423
>500	3.9955268901833117E-4	0.14729011317481414
>1k	1.7666229069160532E-4	0.19523512243060295
>5k	5.888536584350405E-5	0.2360713494247821
>10k+	1.4721341460876012E-5	0.721151967312049
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86842	0.7039840412341616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1349089815156564E-4	0.0	0.0	0.0	0.0
2	1.2159739087667747E-4	0.0	0.0	0.0	8.10649272511183E-6
3	1.2159739087667747E-4	0.0	0.0	8.10649272511183E-6	2.4319478175335494E-5
4	1.2159739087667747E-4	0.0	0.0	8.10649272511183E-6	4.053246362555916E-5
5	1.2970388360178929E-4	0.0	0.0	8.10649272511183E-6	4.863895635067099E-5
6	1.2970388360178929E-4	0.0	0.0	8.10649272511183E-6	5.674544907578282E-5
7	1.2970388360178929E-4	0.0	0.0	8.10649272511183E-6	7.295843452600647E-5
8	1.2970388360178929E-4	0.0	0.0	1.621298545022366E-5	8.917141997623015E-5
9	1.2970388360178929E-4	0.0	0.0	1.621298545022366E-5	9.727791270134198E-5
10	1.3781037632690113E-4	0.0	0.0	2.4319478175335494E-5	1.0538440542645381E-4
11	1.4591686905201295E-4	0.0	0.0	2.4319478175335494E-5	1.1349089815156564E-4
12	1.540233617771248E-4	0.0	0.0	2.4319478175335494E-5	1.3781037632690113E-4
13	2.0266231812779577E-4	8.10649272511183E-6	0.0	2.4319478175335494E-5	1.6212985450223663E-4
14	2.2698179630313128E-4	8.10649272511183E-6	0.0	2.4319478175335494E-5	1.7023634722734845E-4
15	2.5130127447846675E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	1.7023634722734845E-4
16	2.918337381040259E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	1.9455582540268396E-4
17	2.918337381040259E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	2.1887530357801943E-4
18	3.323662017295851E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	2.2698179630313128E-4
19	3.485791871798087E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	2.4319478175335494E-4
20	3.566856799049206E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	2.5130127447846675E-4
21	3.728986653551442E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	2.7562075265380226E-4
22	4.0532463625559155E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	2.9994023082913776E-4
23	4.2964411443092705E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	3.161532162793614E-4
24	4.5396359260626255E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	3.485791871798087E-4
25	4.863895635067099E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	3.485791871798087E-4
26	5.107090416820453E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	3.566856799049206E-4
27	5.431350125824927E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	3.728986653551442E-4
28	5.593479980327163E-4	1.621298545022366E-5	0.0	2.4319478175335494E-5	3.891116508053679E-4
29	5.917739689331637E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	3.9721814353047973E-4
30	5.998804616582755E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.0532463625559155E-4
31	6.404129252838346E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.0532463625559155E-4
32	6.647324034591702E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.0532463625559155E-4
33	7.21477852534953E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.6207008533137437E-4
34	7.539038234354003E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.6207008533137437E-4
35	8.187557652362949E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.701765780564862E-4
36	8.917141997623014E-4	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.944960562318217E-4
37	0.001005205097913867	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.944960562318217E-4
38	0.0010214180833640907	2.4319478175335494E-5	0.0	2.4319478175335494E-5	4.944960562318217E-4
39	0.0010700570397147618	2.4319478175335494E-5	0.0	2.4319478175335494E-5	5.107090416820453E-4
40	0.0011349089815156563	2.4319478175335494E-5	0.0	2.4319478175335494E-5	5.107090416820453E-4
41	0.0012078674160416628	3.242597090044732E-5	0.0	2.4319478175335494E-5	5.350285198573809E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	40590	0.0	41.86462	12
CAACGCA	40765	0.0	41.6849	13
AAGCAGT	41220	0.0	41.298885	1
AACGCAG	41135	0.0	41.252827	14
ACGCAGA	41195	0.0	41.147106	15
ATCAACG	41370	0.0	41.058254	11
GGTATCA	41120	0.0	40.993557	8
GTATCAA	41185	0.0	40.934563	9
GTGGTAT	41410	0.0	40.757545	6
AGTGGTA	41735	0.0	40.412003	5
TGGTATC	41760	0.0	40.393436	7
CGCAGAG	42375	0.0	39.96802	16
AGAGTAC	41350	0.0	39.850544	19
TATCAAC	42350	0.0	39.847343	10
CAGTGGT	44035	0.0	38.47201	4
CAGAGTA	43170	0.0	38.322906	18
GCAGTGG	44905	0.0	37.94644	3
AGCAGTG	44885	0.0	37.93194	2
GCAGAGT	44205	0.0	37.601063	17
GAGTACT	16200	0.0	37.193825	20
>>END_MODULE